BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	3,682,261	Δ34 bp	coding (1154‑1187/1287 nt)	dctA ←	C4 dicarboxylate/orotate:H(+) symporter

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	3682261	3682294	34	70 [0]	[1] 71	dctA	C4 dicarboxylate/orotate:H(+) symporter

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3682260	0 (0.000)	70 (1.010)	61/486	0.2	100%	coding (1188/1287 nt)	dctA	C4 dicarboxylate/orotate:H(+) symporter
*	?	NC_000913	3682295 =	0 (0.000)	70 (1.010)	61/486	0.2	100%	coding (1153/1287 nt)	dctA	C4 dicarboxylate/orotate:H(+) symporter

TGAACTCACCCAGGTGGTAAGTTCAGGCACAGGGGTCAATTATGCGCAAACACCCGCACTCGGGGAAGGGAGTGCGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCCATTGTCAGGTATATCGATGTGCCATCAA  >  NC_000913/3682069‑3682543

acatgaaaatgtcacggaagaagtgaaataaacctgaactcacccaggtggtaagttcaggcacaggggtcaattatgcgcaaacacccgcactcggggaagggagtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCg                                                                                                                                                                                                                                  >  2:322935‑M2/227‑251 (MQ=255)

      aaatgtcacggaagaagtgaaataaacctgaactcacccaggtggtaagttcaggcacaggggtcaattatgcgcaaacacccgcactcggggaagggagtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGAt                                                                                                                                                                                                                            >  1:305461‑M2/221‑251 (MQ=255)

      aaatgtcacggaagaagtgaaataaacctgaactcacccaggaggtaagttcaggcacaggggtcaattatgcgcaaacacccgcactcggggaagggagtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgccaGCGCACGAGCTTCTGACATAACGCGGTCGAt                                                                                                                                                                                                                            >  2:386950‑M2/221‑251 (MQ=255)

        atgtcacggaagaagtgaaataaacctgaactcacccaggtggtaagttcaggcacaggggtcaattatgcgcaaacacccgccctcggggaagggagtgcgggcataagtgatgagattaagaggaaaattcgtgcgttttgacatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttaacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATAc                                                                                                                                                                                                                          >  1:349900‑M2/219‑251 (MQ=255)

          gtcacggaagaagtgaaataaacctgaactcacccaggtggtaagttcaggcacaggggtcaattatgcgcaaacacccgcactcggggaagggagtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCg                                                                                                                                                                                                                        >  1:121748‑M2/217‑251 (MQ=255)

              cggaagaagtgaaataaacctgaactcacacaggtggtaagttcaggcacaggggtcaattatgcgcaaacacccgcactcggggaagggagtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATCCCGaggg                                                                                                                                                                                                                    >  2:486777‑M2/213‑250 (MQ=255)

                   gaagtgaaataaacctgaactcacccaggtggtaagttcaggcacaggggtcaattatgcgcaaacacccgcactcggggaatggagtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtccagtgttttttggtccagttctttcacccacttagcaacgacaGCGCTCGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCaga                                                                                                                                                                                                               <  2:528477‑M2/44‑2 (MQ=255)

                       tgaaataaacctgaactcacccaggtggtaagttcaggcacaggggtcaattatgcgcaaacacccgcactcggggaagggagtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGcc                                                                                                                                                                                                            >  2:495273‑M2/204‑250 (MQ=255)

                       tgaaataaacctgaactcacccaggtggtaagttcaggcacaggagtcaattatgcgcaaacacccgcactaggggaagggagtgcgggcataagtgatgagattaagcggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttggggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGCGGATCAGCGcc                                                                                                                                                                                                            >  2:90391‑M2/204‑250 (MQ=255)

                        gaaataagcctgaactcacccaggtggtaagttcaggcacaggggtcaattatgcgaaaacacccgcacgcggggaaggtagggcgggcataagtgatgagattaagaggataatgcgggctttttgccatccggggcgcgattattcagcaccgcgtccagtgtcttgtggtccagttctgtcacccacttagcaacgacaGTGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAg                                                                                                                                                                                                          <  1:405045‑M2/49‑1 (MQ=255)

                        gaaataaacctgaactcacccaggtggtaagttcaggcacaggggtcaattatgcgcaaacacccgcactcggggaagggagtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGcc                                                                                                                                                                                                            >  1:337527‑M2/203‑249 (MQ=255)

                         aaataaacctgaactcacccaggtggtaagttcaggcacaggggtcaattatgcgcaaacacccgcactcggggaagggagtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatcaggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGCGCTTCTGACATAAAGCAGTCGATACCGAGGATCAGCGcc                                                                                                                                                                                                            >  2:513893‑M2/202‑248 (MQ=255)

                            taaacctgaactcacccaggtggtaagttcaggcacaggggtcaattatgcgcaaacacccgcactcggggaagggagtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGAccc                                                                                                                                                                                                      >  1:643480‑M2/199‑251 (MQ=255)

                              aacctgaactcacccaggtggtaagttcaggcacaggggtcaattatgcgcaaacacccgcactcggggaagggagtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGc                                                                                                                                                                                                    >  1:208842‑M2/197‑251 (MQ=255)

                                 ctgaactcacccaggtggtaagttcaggcaccggggtcaattatgcgcaaacacccgcactcggggaagggagtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGAcc                                                                                                                                                                                                       >  2:454772‑M2/194‑245 (MQ=255)

                                 ctgaactcacccaggtggtaagttcaggcacaggggtcaattatgcgcaaacacccgcactcggggaagggagtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGAcc                                                                                                                                                                                                       <  1:454772‑M2/52‑1 (MQ=255)

                                 ctgaactcacccaggtggtaagttcaggcacaggggtcaattatgcgcaaacacccgcactcggggaagggagtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTAc                                                                                                                                                                                                 >  1:505251‑M2/194‑251 (MQ=255)

                                   gaactcacccaggtggtaagttcaggcacaggggtcaattatgcgcaaacacccgcactcggggaagggagtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCg                                                                                                                                                                                               <  1:56577‑M2/60‑1 (MQ=255)

                                     actcacccaggtggtaagttcaggcacaggggtcaattatgcgcaaacacccgcactcggggaagggagtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGc                                                                                                                                                                                             <  1:341240‑M2/62‑1 (MQ=255)

                                              ggtggtaagttcaggcacaggggtcaattatgcgcaaacacccgcactcggggaagggagtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGAGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGccc                                                                                                                                                                                    >  1:296125‑M2/181‑251 (MQ=255)

                                                 ggtaagttcaggcacaggggtcaattatgcgcaaacccccgcactcggggaagggagtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCAc                                                                                                                                                                                  <  1:292784‑M2/73‑1 (MQ=255)

                                                 ggtaagttcaggcacaggggtcaattatgcgcaaacacccgcactcggggaagggagtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCAcc                                                                                                                                                                                 <  1:579745‑M2/74‑1 (MQ=255)

                                                          aggcacaggggtcaattatgcgcaaacacccgcactcggggaagggagtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGc                                                                                                                                                                        <  2:121748‑M2/83‑1 (MQ=255)

                                                                 ggggtcaattatgcgcaaacacccgcactcggggaagggagtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTcgccg                                                                                                                                                                 >  1:183296‑M2/162‑251 (MQ=255)

                                                                      caattatgcgcaaacacccgcactcggggaagggagtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAAGGGCCCACCGCAGAGAGCGTCGCCGCCAGc                                                                                                                                                            <  2:337527‑M2/95‑1 (MQ=255)

                                                                       aattatgcgcaaacacccgcactcggggaagggagtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAgcc                                                                                                                                                           >  1:138671‑M2/156‑250 (MQ=255)

                                                                       aattatgcgcaaacacccgcactcggggaagggagtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGc                                                                                                                                                            >  2:369245‑M2/156‑250 (MQ=255)

                                                                       aattatgcgcaaacacccgcactcggggaagggagtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGc                                                                                                                                                            >  1:20009‑M2/156‑250 (MQ=255)

                                                                            tgcgcaaacacccgcactaggggaagggagtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGAt                                                                                                                                                       >  1:616679‑M2/151‑250 (MQ=255)

                                                                                 aaacacccgcactcgggggagggagtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttcgcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCCGAGAGCGTCGCCGCCCGCACGATCAAGcc                                                                                                                                                 >  2:356311‑M2/146‑251 (MQ=255)

                                                                                     ccccgcactcggggaagggagtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTa                                                                                                                                             <  1:369245‑M2/110‑1 (MQ=255)

                                                                                              cggggcagggagtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTAcc                                                                                                                                    >  2:421635‑M2/133‑251 (MQ=255)

                                                                                              cggggaagggagtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTa                                                                                                                                      <  1:102863‑M2/117‑1 (MQ=255)

                                                                                               ggggaagggagtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTAccc                                                                                                                                   <  1:698431‑M2/120‑1 (MQ=255)

                                                                                                gggaagggagtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTAcccc                                                                                                                                  >  2:98904‑M2/131‑251 (MQ=255)

                                                                                                     gggagtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTgccg                                                                                                                             >  1:629690‑M2/126‑251 (MQ=255)

                                                                                                         gtgcgggcataagtgatgagattaagaggataattcgtgctttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagtcctttcacccacttagcaacgacaGCGCACGAGCTTCTGCCATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGcccc                                                                                                                         <  2:541222‑M2/130‑1 (MQ=255)

                                                                                                         gtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGcccc                                                                                                                         >  1:548577‑M2/122‑251 (MQ=255)

                                                                                                               gcataagagatgagattaagaggataattcgtgcgttttgccctccggcgcacgattattcagcacatcgtccagttttttgtggtcaagttctttaacccacttagcaacgacaGCGCCCGAGCTTCTGACATACAGCGGTCGATACCGTGGATCAGCGCCAGACCCGCTCCCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCttt                                                                                                                      >  2:272831‑M2/116‑248 (MQ=255)

                                                                                                                 ataagtgatgagattaagaggataattcgttcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGGCGATACCGAGGAGCAGCGCCAGACCCTCTACCGGCAAATGGCCCGCCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTagaag                                                                                                                 <  2:77542‑M2/138‑1 (MQ=255)

                                                                                                                            gattaagaggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAac                                                                                                      <  1:252050‑M2/149‑1 (MQ=255)

                                                                                                                                   aggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTa                                                                                               <  1:298253‑M2/156‑1 (MQ=255)

                                                                                                                                                   tttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGCAGAAAGCAGCAACACGATTAACAGCGTGATTtggtg                                                                               >  2:222570‑M2/80‑251 (MQ=255)

                                                                                                                                                       ccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATCAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACg                                                                           >  1:247788‑M2/76‑251 (MQ=255)

                                                                                                                                                        catccggcgcacgattattaagcacatcttccagttttttgtggtccagctctttcacccacttagcaacgccaGCGCCCGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAACCCGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTCGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGAcgc                                                                          >  2:112993‑M2/75‑250 (MQ=255)

                                                                                                                                                         atccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGat                                                                         <  1:660154‑M2/178‑1 (MQ=255)

                                                                                                                                                                  acgattattcagcacatcgtccagttttttgtggtcaagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTg                                                                >  2:340135‑M2/65‑251 (MQ=255)

                                                                                                                                                                      ttattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTg                                                            >  1:173608‑M2/61‑251 (MQ=255)

                                                                                                                                                                       tattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGt                                                           >  1:424143‑M2/60‑251 (MQ=255)

                                                                                                                                                                        attcagaccatcgtccagttttttggggtccagttctttcacccacttagcaaagacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGAGCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCATCACGATTATCAGCGTGATTTGGTGGACGATATCCATCTGACTGt                                                           <  2:618997‑M2/192‑1 (MQ=255)

                                                                                                                                                                               acatcgtccagttttttgtggtccagttctttcacccacttagcaacgccaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGACAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGCTATCCATCTGACTGTTAGTGGcc                                                   >  1:450602‑M2/52‑251 (MQ=255)

                                                                                                                                                                                catcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCt                                                  <  1:421635‑M2/201‑1 (MQ=255)

                                                                                                                                                                                    gtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCtgggg                                              >  2:177615‑M2/47‑250 (MQ=255)

                                                                                                                                                                                      ccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCg                                             >  1:131902‑M2/45‑250 (MQ=255)

                                                                                                                                                                                         gttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATa                                          >  2:51742‑M2/42‑250 (MQ=255)

                                                                                                                                                                                          ttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGCTACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCCCCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGATGATATCCATCTGACTGTTAGTGGCCTGGGCGATaa                                         >  2:244043‑M2/41‑250 (MQ=255)

                                                                                                                                                                                           tttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCATAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATaaa                                        <  1:555603‑M2/211‑1 (MQ=255)

                                                                                                                                                                                           tttttgtggaccagttctttcacccacttagaaacgacaGCGCTCGAGCTTCTGACATAAAGCGGTCGATACCGTGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGGGCGCGTCGCCGCCAGCACGATAAAGACACTACCCGTTACCCCTGACGCACCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATCTCCATCTGACTGTTAGTGGCCAGGGCGataacc                                       >  2:629058‑M2/40‑249 (MQ=255)

                                                                                                                                                                                                  ggtccagttctgtcacccacttagcaacgccaGCGCACGAGCGTCTAAAAAAAAGCGGTCGATACCGAGGATCAGCGCAAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGCCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGGTATTGGCTTGGGGGATAAACAccgccg                                <  2:616679‑M2/219‑1 (MQ=255)

                                                                                                                                                                                                       agttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGCCTGTTAGTGGCCTGGGCGATAAACACCGCCGCCAtt                           >  2:172676‑M2/28‑251 (MQ=255)

                                                                                                                                                                                                            tttcacccacttagcaccgacaGCGCACGAACTTCTGACATAAAGCGGCCGATACCGAGGATCAGCGCCAGACCCGCTACCGGAAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCCATTGTc                        >  2:444590‑M2/23‑249 (MQ=255)

                                                                                                                                                                                                            tttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCAATTGTc                        >  1:486940‑M2/23‑249 (MQ=255)

                                                                                                                                                                                                               cacccacttagcaacgccaGCGCACGAGATTCTGACCTACAGCGGTCGATACCGAGGCTCAGCGCCAGACCAGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATACAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGCAAGCAGCAACACGCTTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCCATTGTCAGGt                    >  2:353576‑M2/20‑250 (MQ=255)

                                                                                                                                                                                                               cacccacttagcaacgacaGCGCCCGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCCATTGTCAGGta                   <  1:193434‑M2/232‑1 (MQ=255)

                                                                                                                                                                                                                   cacttagcaacgacaGCGAACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCCGCACGATAAAGCCACTACCCGTTACCCCTGCCGCACCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACAGTTAGTGGCCTGGGCGATAAACACCGACGCCATTGTCAGGGATATc               >  2:480985‑M2/16‑251 (MQ=255)

                                                                                                                                                                                                                       tagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGATGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCCATTGTCAGGTATATCGAt            <  1:244043‑M2/239‑1 (MQ=255)

                                                                                                                                                                                                                       tagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATAACGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCCATTGTCAGGTATATCGAtg           >  1:551503‑M2/12‑251 (MQ=255)

                                                                                                                                                                                                                        agccacgacaGCGCGCGATCTTCTGACATAAAGCGGTCTATACCGAGGTTCAGCGCCAGACCCGCTACCGGCAAATGGCCCCCCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCGGGGCGATAAACACCGCCGCCATTGTCAGGTATAGCGAtgt          <  1:386950‑M2/241‑1 (MQ=255)

                                                                                                                                                                                                                        agcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCCATTGTCAGGTATATCGAtgt          <  2:20009‑M2/241‑1 (MQ=255)

                                                                                                                                                                                                                        agcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGGGGCCTGGGCGATAAACACCGCCGCCATTGTCAGGTATATCGAtgt          <  1:42748‑M2/241‑1 (MQ=255)

                                                                                                                                                                                                                                aaGCGCACGAGCTTCGGACTTAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAAGGGCCCACCGCAGAGAGCGTCGCCGCCAGCACTATAAAGCCACTGCCCTTTGCCCCTGCCTCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACCCCGCCGCCATTGTCAGGTATATCGATGTGCCATCaa  <  1:602411/250‑1 (MQ=255)

TGAACTCACCCAGGTGGTAAGTTCAGGCACAGGGGTCAATTATGCGCAAACACCCGCACTCGGGGAAGGGAGTGCGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCCATTGTCAGGTATATCGATGTGCCATCAA  >  NC_000913/3682069‑3682543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`