Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,468,298	IS3 (+) +3 bp	coding (85‑87/921 nt)	yfdH →	bactoprenol glucosyl transferase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2170171 =	NA (NA)	28 (0.590)	26/486	0.7	96.5%	noncoding (1/1258 nt)	IS3	repeat region
	?	NC_000913	= 2468300	1 (0.020)					coding (87/921 nt)	yfdH	bactoprenol glucosyl transferase
*	?	NC_000913	= 2171428	NA (NA)	41 (0.860)	38/484	0.3	100%	noncoding (1258/1258 nt)	IS3	repeat region
	?	NC_000913	2468298 =	0 (0.000)					coding (85/921 nt)	yfdH	bactoprenol glucosyl transferase

GCTACGATAATGCCTGCGTGGAAAGCTTCTTTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTC‑‑TTTAAACCCGACTTCCTGGGTCTCGGTTTCACTATTACCAGACATTATTTTCGCCGCACCGCCATGAAAGCGCGTAACCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAGCACGGTTCCCT  >  NC_000913/2171202‑2171678                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                             gctACGATAATGCCTGCGTGGAAAGCTTCTTTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCTGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAATAACCTCGCTTAGGCCGGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGTATCATTTTg                                                                                                                                                                                                                                      <  2:232928/251‑1 (MQ=255) gctACGATAATGCCTGCGTGGAAAGCTTCTTTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCAtttt                                                                                                                                                                                                                                       <  1:182263/250‑1 (MQ=40)                gCGTGGAAAGCTTCTTTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCt                                                                                                                                                                                                                       <  1:132830/251‑1 (MQ=255)                            tcttTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGttttt                                                                                                                                                                                                            <  2:102267/250‑1 (MQ=255)                               ttCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATAGCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCGCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCTCCTGCGCTATTCTGTTTTTTg                                                                                                                                                                                                          <  2:200702/249‑1 (MQ=255)                                     cGCTGAAAGTTGAATGTATCCATGGAGAACACTGTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCt                                                                                                                                                                                                   <  1:283308/250‑1 (MQ=255)                                                    gTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCg                                                                                                                                                                                    <  2:358312/250‑1 (MQ=255)                                                      aTCCATGGAGAAAACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCTCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGctc                                                                                                                                                                                 <  1:435892/251‑1 (MQ=255)                                                             gagaACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGGTCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTTGCGCGCTGGCGAGCTGAAATCGTTC‑‑TTTAAAc                                                                                                                                                                        >  2:25476/1‑251 (MQ=255)                                                                        aTCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTC‑‑TTTAAACCCGACTTCCTg                                                                                                                                                             <  1:451438/251‑1 (MQ=255)                                                                                    ataatGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTC‑‑TTTAAACCCGACTTCCTGGGTCTCGGTTTc                                                                                                                                                 >  1:264625/1‑251 (MQ=255)                                                                                                          aTTATATCGAATGTGATTACAATCGGTGTCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCGCGCGTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTC‑‑TTTAAACCCGACTGCCTGGGTCTCGGTTTCACTATTTCCAGACATTATTTTc                                                                                                                           <  2:311241/251‑1 (MQ=255)                                                                                                            tataTCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTC‑‑TTTAAACCCGACTTCCTGGGTCTCGGTTTCACTATTACCAGACATTATTTTcgc                                                                                                                         >  1:351586/1‑251 (MQ=255)                                                                                                               atCGAATGTGATTACAATCGGTGTCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTTGCTGCCGCCTGCGCTATTCTGTTTTTTTGCGCGCTGGCGCGCTGAAATCGCTC‑‑TTTTAACCCGCCTTCCTGGGTCTAGGTTTCACTATTTCCAGACATTATTTTcgccgc                                                                                                                      >  1:112119/1‑251 (MQ=25)                                                                                                                        gATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTC‑‑TTTAAACCCGACTTCCTGGGTCTCGGTTTCACTATTACCAGACATTATTTTCGCCGCACCGCCATg                                                                                                             <  2:351586/251‑1 (MQ=255)                                                                                                                              aaTCGGGGGCGGCGGCACAGTTGGTGTGGCGGACTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGGAGGATCAGTCCTCCCTTCGTGGATCCATTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTC‑‑TTTAAACCCGACTTCCTGGGTCTCGGTTTCACTATTACCAGACATTATTTTCGCCGCACCGCCATGAAAgcg                                                                                                       >  1:455977/1‑251 (MQ=255)                                                                                                                                                    ggTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGCCTGCCGCCTGCGCTATGCTGTTTTTTGGCGCGCTGGGGATCTGAAATCGCTC‑‑TTTAAACCCGACTTCCTGGGTCTCGGTTTCACTATTCCCAGACATTATTTTCGCCGCACCGCCATGAAAGCGCGTAACCACGCCTTTTTGTTc                                                                                  <  1:419154/250‑1 (MQ=255)                                                                                                                                                                   gTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTC‑‑TTTAAACCCGACTTCCTGGGTCTCGGTTTCACTATTACCAGACATTATTTTCGCCGCACCGCCATGAAAGCGCGTAACCACGCCTTTTTGTTCGAGAAAGCGCAAATCg                                                                  <  1:132505/251‑1 (MQ=255)                                                                                                                                                                                     aCAAGAACCTCGCTTAGGCCTGTTTCCATTTTCCGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGGGCGCTGGCGAGCTGAAATCGCTC‑‑TTTAAACCCGACTTCCTGGGTCTCGGTTTCACTATTACCAGACATTATTTTCGCCGCACCGCCATGAAAGCGCGTAACCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTcc                                                <  2:264625/251‑1 (MQ=255)                                                                                                                                                                                                    aGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTC‑‑TTTAAACCCGACTTCCTGGGTCTCGGTTTCACTATTACCAGACATTATTTTCGCCGCACCGCCATGAAAGCGCGTAACCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACt                                 >  2:133597/1‑251 (MQ=255)                                                                                                                                                                                                                     gcgggggTAGGATCTGTCAGCCCTTCGGGGATCATTTTGACGGCCGCCTGCGTTATTCTGTTTTTGGGCGCGCGGGCGAGCTGAAATCGCTC‑‑TTTAACCCCGCCTTCCTGGTCCTGGGTTTCACTATTACCAGACATTTTTTTCGCCGCACCGCCATGAAAGCGCGTAACCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATTCTCCCGCCAGATCCTGAAc                <  2:124097/247‑1 (MQ=255)                                                                                                                                                                                                                               gtacAGTCATCCCTTTGTGATTCATTTTGTCTGCCGCTTGCCTTATTCTTTTTTTTGGCGCGCTGTGGAGCT‑AAATC‑CTCCTTTTAAACCCGTCTTCCTGGGTCCTGGTTTCACAATTACCAGACATTATTTTCGCCGCACCGCCATGAAAGCGCGTAACCACGCCTTTGTGTTCGAGAAAGCTCAAATCGGCACGGATTGCCGCTGCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAGCACGGtt      <  1:40243/248‑1 (MQ=255)                                                                                                                                                                                                                                   aGTCATCCTTTCGTGGATCATTGTGACGGCCGCCTGCGCTATGCTGTTTTTTGGGGCGCTGGCGAGCTGAAATCGCTC‑‑TTTAAACCGGACGTCCTGGGTCGCGGTTTCACTATTGCCAGAAATTATTTCCGCCGCACCGCCATGAAAGCGCGTAACCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAGCACGGTTCCCt  <  1:50656/251‑1 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                             GCTACGATAATGCCTGCGTGGAAAGCTTCTTTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTC‑‑TTTAAACCCGACTTCCTGGGTCTCGGTTTCACTATTACCAGACATTATTTTCGCCGCACCGCCATGAAAGCGCGTAACCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAGCACGGTTCCCT  >  NC_000913/2171202‑2171678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.