Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,468,298	IS3 (+) +3 bp	coding (85‑87/921 nt)	yfdH →	bactoprenol glucosyl transferase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2170171 =	NA (NA)	28 (0.590)	26/486	0.7	96.5%	noncoding (1/1258 nt)	IS3	repeat region
	?	NC_000913	= 2468300	1 (0.020)					coding (87/921 nt)	yfdH	bactoprenol glucosyl transferase
*	?	NC_000913	= 2171428	NA (NA)	41 (0.860)	38/484	0.3	100%	noncoding (1258/1258 nt)	IS3	repeat region
	?	NC_000913	2468298 =	0 (0.000)					coding (85/921 nt)	yfdH	bactoprenol glucosyl transferase

ACCATCAGCACCGACAAACATTAAATCGGCATTAATATCCTGTAATGAACGCTCGGCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATCAGCAGACGT  >  NC_000913/2169927‑2170410                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                aCCATCAGCACCGACAAACATTAAATCGGCATTAATATCCTGTAATGAACGCTCGGCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCtt                                                                                                                                                                                                                                           >  1:357906/1‑251 (MQ=255)      cAGCACCGACAAACATTAAATCGGCATTAATATCCTGTAATGAACGCTCGGCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTTGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAAAGGTCTGATCTTAccc                                                                                                                                                                                                                                       >  1:272599/1‑250 (MQ=255)         cACCGACAAACAGTAAAGCGGCATTAATATCCTGTAATGAACGCTCGGCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATTTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTGGGTAATCACCGTGATATTTTTGGCTGTCAGTAATCCTGCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCCTAACCAGCa                                                                                                                                                                                                                                   <  2:408304/251‑1 (MQ=255)           ccGACAAACATTAAATCGGCATTAATATCCTGTAATGAACGCTCGGCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCCGCAAt                                                                                                                                                                                                                                 >  2:374654/1‑251 (MQ=255)               cAAACCTTTAATCGGCATTAATATCCTGTAATGAACGTTCGGCAATGGAACCATGCATCGAGTGCGTGTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGTAAACGCTGCCGGGAGACTGTTGTTAATCACCGTGATATTTTTGGCGGGCAGTAATCCGTCGGCGATAAGCATTGTCGTACGTCCGCTGTCGAGAATAACGGTCTGATCTTCCCCAGCACTAGTg                                                                                                                                                                                                                             <  2:283301/251‑1 (MQ=255)                                                         cAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCTGAAAGGGCAAAAGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTAATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTAGACAAGCGGCTAAGTGAGTAACATCTCAGTCAGAGGTGACTc                                                                                                                                                                                   >  1:302801/1‑251 (MQ=255)                                                                     gCATCGAGCGCGTTTTATGGCGGACCGTGCCACCTCAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACaaaaa                                                                                                                                                                       <  1:176023/251‑1 (MQ=255)                                                                            gcgcGTTTTATGGCGGACCGTGCCCCCACAGTCGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGGAATCGCCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTGACCCAGCAATAGTGGACACTCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATc                                                                                                                                                                <  2:123166/251‑1 (MQ=255)                                                                                     aTGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATAGTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTa                                                                                                                                                        <  2:419233/250‑1 (MQ=255)                                                                                         cGGACCGTGCCACCACAGACGACCAGGGTAATGTCTTTATTGTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTAAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTaaaaaa                                                                                                                                                   <  2:498824/251‑1 (MQ=255)                                                                                              cGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCg                                                                                                                                              >  1:269460/1‑251 (MQ=255)                                                                                                    accacAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCt                                                                                                                                                                                                                                                 <  2:142384/146‑1 (MQ=255)                                                                                                    accacAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCt                                                                                                                                                                                                                                                 >  1:142384/1‑146 (MQ=255)                                                                                                    accacAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAAc                                                                                                                                         >  2:484664/1‑250 (MQ=255)                                                                                                       acaGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCa                                                                                                                                     <  2:29158/251‑1 (MQ=255)                                                                                                            cgacCAGAGAAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGc                                                                                                                                >  2:370803/1‑251 (MQ=255)                                                                                                                         gTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAg                                                                                                                   <  2:335772/251‑1 (MQ=255)                                                                                                                              tATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTTAATTTCGCAGTGa                                                                                                                >  2:356956/1‑249 (MQ=255)                                                                                                                                   tCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCt                                                                                                          <  1:413153/250‑1 (MQ=255)                                                                                                                                       aaaGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGAGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGAGAAGACCTGAAGc                                                                                                     >  2:258200/1‑251 (MQ=255)                                                                                                                                        aaGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCt                                                                                                    >  2:69087/1‑251 (MQ=255)                                                                                                                                         aGGGCAAACGCTGCCGAGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTACGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACCCGAGGCTAAGTGAGTAAACTCTCAGTCAGCGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAACCTTCACCTGAATTTAGAACTGAAGCCATGAAGCtt                                                                                                   >  2:189831/1‑251 (MQ=25)                                                                                                                                                  gCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTTCTGAACGc                                                                                          >  2:242182/1‑251 (MQ=255)                                                                                                                                                    tGCCGGGAGACTGTTGGTAATAACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACCCGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGCATCAACCAGTAAAAAACCCCGAAACCAGCATTCCCCTGAATTTCGCCGCGAAACCCTGCAGCTTGCTGAACGCAt                                                                                        >  2:223231/1‑251 (MQ=21)                                                                                                                                                                     tAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGc                                                                         >  1:5809/1‑249 (MQ=255)                                                                                                                                                                               gATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAAc                                                              <  1:69087/250‑1 (MQ=255)                                                                                                                                                                               gATATTTTTGGCGGTCATTAATCCTTCGGAGATAAGCATTGTCGTACTTCCGCCGTCGAGAATAACGGTCTGATCTTACCCAGCACTAGTGGACAAGCGGCTAAGTGCGTAAACTCTCAGGCAGAGGTGACTAACATGACAAAAACAGTATCAACCAGTAAAAAACCACGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTGGCTGCACGCATCGGTGTTACTGACGCAGCCCGTGAACt                                                             >  2:175616/1‑251 (MQ=18)                                                                                                                                                                                     tttGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACCCGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGAGTTACTGCCGCAGACCGTGAACTCAGCCt                                                       >  1:414070/1‑251 (MQ=255)                                                                                                                                                                                        ggCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTa                                                    <  2:371573/251‑1 (MQ=255)                                                                                                                                                                                                 tAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATACCGGTCTGATCTTACCCAGCAATAATGGACACGCGGCTAAGTGAGTACACTCTCAGTCAGAGGAGCCTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATcac                                            >  1:79777/1‑250 (MQ=255)                                                                                                                                                                                                 tAATCCTACGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGTTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCTTGTATGAATcac                                            <  2:414070/250‑1 (MQ=255)                                                                                                                                                                                                                                       cGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACGAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATCAGCAGa     <  1:403442/251‑1 (MQ=21)                                                                                                                                                                                                                                         agaATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATCAGCAGACg   <  1:304842/251‑1 (MQ=33)                                                                                                                                                                                                                                          gaATAGCGGTCTGATCTTACCCAGCAATAGTGGACACTCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACACAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTAGCCTGAATTTCGCAGTGAAGCCCTGCAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATCAGCAGACGt  >  2:98236/1‑251 (MQ=9)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                ACCATCAGCACCGACAAACATTAAATCGGCATTAATATCCTGTAATGAACGCTCGGCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATCAGCAGACGT  >  NC_000913/2169927‑2170410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.