Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	4,147,156	IS2 (–) +5 bp	coding (19‑23/342 nt)	frwD →	putative PTS enzyme IIB component FrwD

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	4147156 =	0 (0.000)	61 (0.980)	57/488	0.2	100%	coding (19/342 nt)	frwD	putative PTS enzyme IIB component FrwD
	?	NC_000913	4498181 =	NA (NA)					noncoding (1/1331 nt)	IS2	repeat region
*	?	NC_000913	= 4147160	0 (0.000)	117 (1.880)	98/490	0.0	100%	coding (23/342 nt)	frwD	putative PTS enzyme IIB component FrwD
	?	NC_000913	= 4499511	NA (NA)					noncoding (1331/1331 nt)	IS2	repeat region

GGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATT  >  NC_000913/4499275‑4499538                                                                                                                                                                                                                                             |                            ggAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGAGTTCGCGCATTATAACGCATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGGGATAACAGATGTCTGGAAATATAGGGGCAAATCCAcccaggtagtg                  >  2:327805/1‑239 (MQ=11)              atagatgagatCTTCGTGAAAACGATAAAGCGTGACTACATCAGTAGCATGCCCAAAACAGATGGGTTAACGGCAGCAAAGAACCTTGCATAGGCGTTGGAGCATTAGAACGAAGTGCAGCAGCAGAGGGCGCGGGGTTATCGCTTGCCACGGTAATGTCTGTGGCAGCGTGCATGTAAGTGTTTAAGTGATAATAGAGGTCATGAAATGTAGGGGCAATTCCTCCTTGAGCTGTTACGAATACGGATAtt  <  2:611180/245‑1 (MQ=2)                                                                                                                                                                                                                                             |                            GGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATT  >  NC_000913/4499275‑4499538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.