Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	4,147,156	IS2 (–) +5 bp	coding (19‑23/342 nt)	frwD →	putative PTS enzyme IIB component FrwD

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	4147156 =	0 (0.000)	61 (0.980)	57/488	0.2	100%	coding (19/342 nt)	frwD	putative PTS enzyme IIB component FrwD
	?	NC_000913	4498181 =	NA (NA)					noncoding (1/1331 nt)	IS2	repeat region
*	?	NC_000913	= 4147160	0 (0.000)	117 (1.880)	98/490	0.0	100%	coding (23/342 nt)	frwD	putative PTS enzyme IIB component FrwD
	?	NC_000913	= 4499511	NA (NA)					noncoding (1331/1331 nt)	IS2	repeat region

CGCAGGAATGCGTAGATGCTGCATTGCACATAGCGGCAATGTTCAAATCGCTCGGCACCGGCAAGCTCGATATCCGTAATCAACAGAGCAACATCCGCCCGGCGGATATCCTCATCCGCTAAACGATTCTCCGTTCCCAGCGCGCCCTGAGTTTCAATGCTGACTCCCCACTTCTCTAACAGGCACAACTTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTACt‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4147403‑4147156 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TAGACTGGCCCCCTGAATCT‑CCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCT  >  NC_000913/4498181‑4498414                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     CGCAGGAATGCGTAGATGCTGCATTGCACATAGCGGCAATGTTCAAATCGCTCGGCACCGGCAAGCTCGATATCCGTAATCAACAGAGCAACATCCGCCCGGCGGATATCCTCATCCGCTAAACGATTCTCCGTTCCCAGCGCGCCCTGAGTTTCAATGCTGACTCCCCCCTTCTCTAACAGGCACAACTTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTACTAG                                                                                                                                                                                                                                          <  2:534393/251‑1    AGGAATGCGTAGATGCTGCATTGCACATAGCGGCAATGTTCAAATCGCTCGGCACCGGCAAGCTCGATATCCGTAATCAACAGAGCAACATCCGCCCGGCGGATATCCTCATCCGCTAAACGATTCTCCGTTCCCAGCGCGCCCTGAGTTTCAATGCTGACTCCCCACTTCTCTAACAGGCACAACTTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTACTAGACT                                                                                                                                                                                                                                       >  1:572191/1‑251                ATGCTGCATTGCACATAGCGGCAATGTTCAAATCGCTCGGCCCCGGCAAGCTCGATATCCGTAATCAACAGAGCAACATCCGCCCGGCGGATATCCTCATCCGCTAAACGATTCTCCGTTCCCAGCGCGCCCTGAGTTTCAATGCTGACTCCCCACTTCTCTAACAGGCACACCTTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTACTAGACTGGCCCCCTGAAT                                                                                                                                                                                                                           <  2:402104/251‑1                    cctcccgatctTAGCGGCAATGTTCAAACCGCTCGGCACCGGCAAGCTCGATATCCGTAATCAACAGAGCAACATCCGCCCGGCGGATATCCTCATCCGCTAAACGATTCTCCGTTCCCAGCGCGCCCTGAGTTTCAATGCTGACTCCCCACTTCTCTAACAGGCACAACTTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTACTAGACTGGCCCCCTGAATCT‑CC                                                                                                                                                                                                                      <  2:467697/240‑1                            ACATAGCGGCAATGTTCAAATCGCTCGGCACCGGCAAGCTCGATATCCGTAATCAACAGAGCAACATCCGCCCGGCGGATATCCTCACCCGCTAAACGATTCTCCGTTCCCAGCGCGCCCTGAGTTTCAATGCTGACTCCCCACTTCTCTAACAGGCACAACTTTGCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTACTAGACTGGCCCCCTGAATCT‑CCAGACAACC                                                                                                                                                                                                              <  2:41695/251‑1                               TAGCGGCAATGTTCAAATCGCTCGGCACCGGCAAGCTCGATATCCGTAATCAACAGAGCAACATCCGCCCGGCGGATATCCTCATCCGCTAAACGATTCTCCGTTCCCAGCGCGCCCTGAGTTTCAATGCTGACTCCCCACTTCTCTAACAGGCACAACTTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTACTAGACTGGCCCCCTGAATCT‑CC                                                                                                                                                                                                                      >  1:467697/1‑240                                AGCGGCAATGTTCAAATCGCTCGGCACCGGCAAGCTCGATATCCGTAATCAACAGAGCAACATCCGCCCGGCGGATATCCTCATCCGCTAAACGATTCTCCGTTCCCAGCGCGCCCTGAGTTTCAATGCTGACTCCCCACTTCTCTAACAGGCACAACTTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTACTAGACTGGCCCCCTGAATCT‑CCAGAAAACCcat                                                                                                                                                                                                           >  1:183167/1‑247                                  CGGCAATGTTCAAATCGCTCGGCACCGGCAAGCTCGATATCCGTAATCAACAGAGCAACATCCGCCCGGCGGATATCCTCATCCGCTAAACGATTCTCCGTTCCCAGCGCGCCCTGAGTTTCAATGCTGACTCCCCACTTCTCTAACAGGCACAACTTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTACTAGACTGGCCCCCTGAATCT‑CCAGACAACCAATATC                                                                                                                                                                                                        <  1:13907/251‑1                                  CGGCAATGTTCAAATCGCTCGGCACCGGCAAGCTCGATATCCGTAATCAACAGAGCAACATCCGCCCGGCGGATATCCTCATCCGCTAAACGATTCTCCGTTCCCAGCGCGCCCTGAGTTTCAATGCTGACTCCCCACTTCTCTAACAGGCACAACTTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTACCAGACTGGGCCCCTGCATCTTCCAG‑CAACCAATAT                                                                                                                                                                                                         >  2:213317/1‑250                                  CGGCAATGTTCAAATCGCTCGGCACCGGCAAGCTCGATATCCGAAATCAACAGAGCAACATCCGCCCGGCGGATATCCTCATCCGCTAAACGATTCTCCGTTCCCAGCGCGCCCTGAGTTTCAATGCTGACTCCCCACTTCTCTAACAGGCACAACTTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTACTAGACTGGCCCCCTGAATCT‑CCAGACAACCAATATC                                                                                                                                                                                                        <  1:134186/251‑1                                    GCAATGTTCAAATCGCTCGGCACCGGCAAGCTCGATATCCGTAATCAACAGAGCAACATCCGCCCGGCGGATATCCTCATCCGCTAAACGATTCTCCGTTCCCAGCGCGCCCTGAGTTTCAATGCTGACTCCCCACTTCTCTAACAGGCACAACTTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTAATAAACTTGCCCCCTGACTCT‑CCAGACAACCAAAATCAC                                                                                                                                                                                                      >  1:51490/1‑251                                     CAATGTTCAAATCGCTCGGCACCGGCAAGCTCGATATCCGTAATCAACAGAGCAACATCCGCCCGGCGGATATCCTCATCCGCTAAACGATTCTCCGTTCCCAGCGCGCCCTGAGTTTCAATGCTGACTCCCCACTTCTCTAACAGGCACAACTTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTACTAGACTGGCCCCCTGAATCT‑CCAGACAACCAATATCACT                                                                                                                                                                                                     <  1:22575/251‑1                                       ATGTTCAAATCGCCCGGCACCGGCAAGCTCGATATCCTTAATCAACAGACCACCATCCGCCCGGCGGATATCCTCATCCGCTAACCGATTCTCCGTTCCCAGCGCGCCCTGAGTTTCAATGCTGACTCCCCACTTCTCTAACAGGCCCAACTTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTTCTAGACTGGCCCCCTGAATCT‑CCAGACAACCAATATCACTTA                                                                                                                                                                                                   <  2:465967/251‑1                                               ATCGCTCGGCACCGGCAAGCTCGATATCCGTAATCAACAGAGCAACATCCGCCCGGCGGATATCCTCATCCGCTAAACGATTCTCCGTTCCCAGCGCGCCCTGAGTTTCAATGCTGCCTCCCCACTTCTCTAACAGGCACAACTTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTACTAGACTGGCCCCCCGAATCT‑CCCAACAACCAATATCACTTAAATAAGTG                                                                                                                                                                                           >  2:402204/1‑251                                                 CGCTCGGCACCGGCAAGCTCGATATCCGTAATCAACAGAGCAACATCCGCCCGGCGGATATCCTCATCCGCTAAACGATTCTCCGTTCCCAGCGCGCCCTGCGTTTCAATGCTGACTCCCCACTTCTCTAACAGGCACAACTTTTCCAGCCGTTCCGCCGCCATATCAGTATGCGCCACGCCGCTTACGCAGGCGGTTTCTTAACTGGCCCCCTGCATCT‑CCCGCAAACCACTATCACTTtaaaaaatga                                                                                                                                                                                          >  2:257006/1‑240                                                       GCACCGGCAAGCTCGATATCCGTAATCAACAGAGCAACATCCGCCCGGCGGATATCCTCATCCGCTAAACGATTCTCCGTTCCCAGCGCGCCCTGAGTTTCAATGCTGACTCCCCACTTCTCTAACAGGCACAACTTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTACTAGACTGGCCCCCTGAATCT‑CCAGACAACCAATATCACTTAAATAAGTGATAGTCTT                                                                                                                                                                                   <  1:494275/251‑1                                                         ACCGGCAAGCTCGATATCCGTAATCAACAGATCAACATCCGCCCGGCGGAGATCCTCATCCGCTAAACGATTCTCCGTTCCCAGCGCGCCCTGAGTTTCAATGCTGACTCCCCACTTCTCTAATAGGCACAACGTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGGCACGCCGCTTACGCAGGCGGTTACTAGACTGGCCCCCTGAATCT‑CCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAA                                                                                                                                                                                 <  1:616905/251‑1                                                                AGCTCGATATCCGTAATCAACAGAGCAACATCCGCCCGGCGGATATCCTCATCCGCTAAACGATTCTCCGTTCCCAGCGCGCCCTGAGTTTCAATGCTGACTCCCCACTTCTCTAACAGGCACAACTTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTACTAGACTGGCCCCCTGAATCT‑CCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGT                                                                                                                                                                          <  1:327578/251‑1                                                                  CTCGATATCCGTAATCAACAGAGCAACATCCGCCCGGCGGATATCCTCATCCGCTAAACGATTCTCCGTTCCCAGCGCGCCCTGAGTTTCAATGCTGACTCCCCACTTCTCTAACAGGCACAACTTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTACTAGACTGGCCCCCTGAATCT‑CCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTT                                                                                                                                                                        >  1:386953/1‑251                                                                     GATATCCGTAATCAACAGAGCAACATCCGCCCGGCGGATATCCTCATCCGCTAAACGATTCTCCGTTCCCAGCGCGCCCTGAGTTTCAATGCTGACTCCCCACTTCTCTAACAGGCACAACTTTTCCAGCCGTTCCGCCGCCCTATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTTCTAGACTGGCCCCCTGAATCT‑CCAGGCAACCAAAATAACTTAAAAAAATGAAAGTCTTTATAACAGTTTTT                                                                                                                                                                      >  1:207007/1‑250                                                                      ATATCCGTAATCAACAGAGCAACATCCGCCCGGCGGATATCCTCATCCGCTAAACGATTCTCCGTTCCCAGCGCGCCCTGAGTTTCAATGCTGACTCCCCACTTCTCTAACAGGCACAACTTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTACTAGACTGGCCCCCCGAATCT‑CCAGACAACCAATATCACTTAAATAAGGGATAGTCTTAATACTAGTTTTT                                                                                                                                                                      >  1:20644/1‑249                                                                        ATCCGTAATCAACAGAGCAACATCCGCCCGGCGGATATCCTCATCCGCTAAACGATTCTCCGTTCCCAGCGCGCCCTGAGTTTCAATGCTGACTCCCCACTTCTCTAACAGGCACAACTTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTCCTCGACTGGCCCCCCGCCTCT‑CCAGACAACCCATATCACTTATATAAGTGGTATTCTTAATACTAGTTTTTAGA                                                                                                                                                                   >  2:456022/1‑250                                                                              AATCAACAGAGCAACATCCCCCCGGCGGATATCCTCATCCGCTAAACGATTCCCCGTTCCCAGCGCGCCCTGAGTTTCAAGGCTGCCTCCCCACTTCTCTAACAGGCCCAACTTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTACTAGACTGGCCCCCTGAATCT‑CCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAAGACTAGTTTTTAGACTAGTCA                                                                                                                                                            <  2:63334/251‑1                                                                                  AACAGAGCAACATCCGCCCGCCGGATATCCTCATCCGCTAAACGATTCTCTGTTCCCAGCGCGCCCTGAGTTGCAATGCTGACTCCCCACTTCTCTAACAGGCACAACTTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTACTAGACTGGCCCCCTGAATCT‑CCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGG                                                                                                                                                        <  1:443322/251‑1                                                                                        GCAACATCCGCCCGGCGGATATCCTCATCCGCTAAACGATTCTCCGTTCCCAGCGCGCCCTGAGTTTCAATGCTGACTCCCCACTTCTCTAACAGGCACAACTTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTCCTAGACTGGCCCCCCGAATCT‑CCAGACAACAAATATCACTTAAATAAGGGAAAGTCTTAATACTAGTTTTTAGACTAGTAATTGGAGAAC                                                                                                                                                   >  2:152831/1‑250                                                                                        GCAACATCCGCCCGGCGGATATCCTCATCCGCTAAACGATTCTCCGCTCCCACCGCGCCCTGAGTTTCAATGCTGACACCCCACTTCTCTAACAGGCACACCTTTTCCAGCCCTTCCGCCGCCATATACGTATGCGCCACCCCGCTTACGCAGGCGGTTACCAGACTGTCCCCCTGAATCT‑CCACACCACCCATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTATACTAGTCATTGGCGAACc                                                                                                                                                  >  1:288118/1‑250                                                                                         CAACAGCCGCCCGGCGGATATCCTCATCCGCTAAACGATTCTCCGTTCCCAGCGCGCCCTGAGTTTCAATGCTGACTCCCCACTTCTCTAACAGGCACAACTTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTACTAGACTGGCCCCCTGAATCT‑CCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAG                                                                                                                                                 <  1:48157/251‑1                                                                                          AACATCCGCCCGGCGGATGTCCTCATCTGCTAAACGATTCTCCGTTCACAGCGCGCCCTGAGTTTCAATGCTGACTCCCCACTCCTCTAACAGGCACAACATTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGAGGTTACTAGACTGGCCCTCTGAATCT‑CCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGTCTAGTCATTGGAGAACAGA                                                                                                                                                <  2:186503/251‑1                                                                                            CATCCGCCCGGCGGATATCCTCATCCGCTAAACGATTCTCCGTTCCCAGCGCGCCCTGAGTTTCAATGCTGACTCCCCACTTCTCTAACAGGCACAACTTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTACTAGACTGGCCCCCTGAATCT‑CCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATG                                                                                                                                              >  1:91328/1‑251                                                                                            CATCCGCCCGGCGGATATCCTCATCCGCTAAACGATTCTCCGTTCCCAGCGCGCCCTGAGTTTCAATGCTGACTCCCCACTTCTCTAACAGGCACAACTTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTACTAAACTGGCCCCCTGAATCT‑CCCGACAACCAATATCAATTAAATAAATGATAGTCTTAATACTAGTTTTTTGACTAGTCATTGGGGAACAGATG                                                                                                                                              >  1:183720/1‑251                                                                                             ATCCGCCCGGCGGATATCCTCATCCGCTAAACGATTCTCCGTTCCCAGCGCGCCCTGAGGTTCAATGCTGACTCCCCACTTCTCTAACAGGCACAACTTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTACTAGACTGGCCCCCTGAAACT‑CCAGACAACCACTATCACTTAAATACGATCGAGTCTTAATACCCATTTTTATGCTAGTCAATGGAGAACCGATGt                                                                                                                                             >  2:10928/1‑250                                                                                                                         AAACGATTCTCCGTTCCCAGCGCGCCCTGAGTTTCAATGCTGACTCCCCACTTCTCTAACAGGCACAACTTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTACTAGACTGGCCCCCTGCATCT‑CCAGACAAACAATATCACTTAAATAAGGGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGGGAAACGC                                                                                                                   >  1:216480/1‑249                                                                                                                                     GTTCCCAGCGCGCCCTGAGTTTCAATGCTGACTCCCCACTCCTCTAACAGGCACAACTTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTACTAGACTGGCCCCCTGAATCT‑CCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCAC                                                                                                     <  2:568811/251‑1                                                                                                                                          CAGCGCGCCCTGAGTTTCAAGGCTGACTCCCCACTTCTCTAACAGGCACACCTTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTACTAGACTGGCCCCCTGAATCT‑CCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGG                                                                                                <  2:113408/251‑1                                                                                                                                                GCCCTGAGTTTCAATGCTGACTCCCCACTTCTCTAACAGGCACAACTTTTCCAGCCGTTCCGCCGCCATATACGTATGCGCCACGCCGCTTACGCAGGCGGTTACTAGACTTGCCCCCTGAATCC‑TCAGACAACCAATCTCACTTAAATAAGTGGTAGTCTTTATACTAGTTTTTAGACTAGTCCTTTGAGAACAGATTATTGATGTCTTAGGGCCGGGGAAACGCAGACGGCGTACCACACAGGAAA                                                                                             >  2:32554/1‑248                                                                                                                                                    TGAGTTTCAATGCTGACTCCCAACTTCTCTAACAGGCACAACTTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTAATAGAATGGCCCCCTGAATCT‑CCAGACAACCAATATCACTTAAATAAGTGATAATATTAATACTAGTTTTTAGACTAGGCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGGACCACACAAGAAAAGAACGC                                                                                      >  1:498018/1‑251                                                                                                                                                     gatTTTCAATGCTGACTCCCCACTTCTCTAACAGGCACAACTTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTACTAGACTGGCCCCCTGAATCT‑CCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGAGTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCG                                                                                       <  2:266424/246‑1                                                                                                                                                       GTTTCAATGCTGACTCCCCACTTCTCTAACAGGCACAACTTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTACTAGACTGGCCCCCTGAATCT‑CCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAAT                                                                                   <  1:257006/251‑1                                                                                                                                                           caagtCTGACCCCCCCCTTCTCTAACAGGCCCACCTTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTACTAGACTGGCCCCCTGAATCT‑CCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCAGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTT                                                                               <  2:361196/246‑1                                                                                                                                                                  GACTCCCCACTTCTCTAACAGGCACAACTTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTACTAGACTGGCCCCCTGAATCT‑CCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCAGTGGAGAACAGATGATTGATGTCGTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCA                                                                          <  2:278465/249‑1                                                                                                                                                                    CTCCCCACTTCTCTAACAGGCACAACTTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTACTAGACTGGCCCCCTGAATCT‑CCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCCTTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAAAGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGC                                                                      >  1:254880/1‑251                                                                                                                                                                     TCCCCACTTCTCTAACAGGCACAACTTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTTCTAGACTGGCCCCCTGAATAT‑CCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATTTCTTAGGGCCGGAGAAACGCAACCGGCGTACCACACAGGAA                                                                                              >  2:233351/1‑226                                                                                                                                                                     TCCCCACTTCTCTAACAGGCACAACTTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTACTAGACTGGCCCCCTGAATCT‑CCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAA                                                                                              <  1:233351/226‑1                                                                                                                                                                        CCACTTCTCGAACAGGCACACCTTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTACTAGACTGGCCCCCTGAATCT‑CCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCAGTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAAGTGTTCAGCAGAGCTTTG                                                                  <  2:522581/251‑1                                                                                                                                                                        CCACTTCGCTAACAGGCACAACTTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTACTAGACTGGCCCCCTGAATCT‑CCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTG                                                                  <  1:102710/251‑1                                                                                                                                                                          ACTTCTCTAACAGGCACAACTTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTACTAGACTAGCCCCCTGAAACT‑CCAGACAACCACTATCACTTTAATAAGTGATAGTCTTAATACTAGTTTTTAGAATAGTCATTGGAGAACAGATGATTGATGTCTTTAGGCCGGAGAAACGCAGACGGCGTACCACACAGGGAAAGATCGCCATTGTTACGCAGAGCTTTGA                                                                 >  1:523284/1‑250                                                                                                                                                                               TCTAACAGGCACAACTTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTACTAGACTGGCCCCCTGAATCT‑CCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGAGTACCCCACAGAAAAAGATCGCAATTGTTCAGCAGAGCTTTTAACCGGG                                                           >  2:471683/1‑251                                                                                                                                                                                  AACAGGCACAACTTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTACTAAACTGGCCCCCTGAATCT‑CCAGACAACCCATATCAATTTAATAAGTGATAGTCTTAATAATAGTTTTTAGACTAGTCCTTGGAGAACAGATGATTGATGTCTTAGGGCCGGGGAAAAGCAGACGGCGTACCACACAGGAAAAAATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGAT                                                        >  1:228976/1‑251                                                                                                                                                                                   ACAGGCACAACTTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTACTAGACTGGCCCCCTGAATCT‑CCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTAATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAAAGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCCGAGCTTTGAACCGGGGATG                                                       >  2:193523/1‑251                                                                                                                                                                                   ACAGGCACAACTTTACCAGACGTTCCGCCGCCATATAAGTATTCGCCACGCCGCTTACGCAAGCGGTTACTAGACTGGCCCCCTGAATCT‑CCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGCCATTGGAGAACAGATGAGTGATGTCCTAGGGCCGTAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGATTTGAACCGGGGATG                                                       <  2:307074/251‑1                                                                                                                                                                                                        tTTCCTCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTACTAGACTGGCCCCCTGAATCT‑CCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCG                                   <  1:437008/249‑1                                                                                                                                                                                                           CCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTACTAGACTGGCCCCCTGAATCT‑CCCGACAACCAATATCACTTAAATAAGGGATAGTCTTAATAATAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTTAGCAGAGCTTTGGACCGGGGGTGACGGGCTCCCTCGTTGCCCGGCc                                >  2:162593/1‑249                                                                                                                                                                                                            CGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTACTAGACTGGCCCCCTGAATCT‑CCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGAAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAAC                              <  1:23285/251‑1                                                                                                                                                                                                                 CCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTACTAGACTGGCCCCCTGAATCT‑CCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACCTGGg                         >  1:261349/1‑250                                                                                                                                                                                                                     ATAAGTAGGCGCCACGCCGCTTACGCAGGCGGTTACTAGACTGGCCCCCTGAATCT‑CCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGGTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTA                      <  2:348059/250‑1                                                                                                                                                                                                                         GTATGCGCCACGCCGCTTACGCAGGCGGTTACTAGACTGGCCCCCTGAATCT‑CCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGC                 <  1:331799/251‑1                                                                                                                                                                                                                           ATGCGCCACGCCGCTTACGCAGGCGGTTACTAGACTGGCCCCCTGAATCT‑CCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCC                <  1:358924/250‑1                                                                                                                                                                                                                               GCCACGCCGCTTACGCAGGCGGTTTCTAGAATGGCCCCCCGGATCT‑CCAGCCAACCAATATCACTTAAATAAGTGATAGTCTTAATACCAGTTTTTAGACTAGTCATTGGAGAACAGATGGTTGATGTCTTAGGGCCGGGGAAACGCAGACGGCGTACCCCACAGGGAAAGATCGCAATTGTTCAGCAGAGCTTTTAACCGGGGGTGACGGGCTCCCTCGTTGGCCGGCAACATGGGGTCGCCGCCAGCC            >  2:387073/1‑250                                                                                                                                                                                                                                 CACGCCGCTTACGCAGGCGGTTACTAGACTGGCCCCCTGAATCT‑CCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGT         <  1:546922/251‑1                                                                                                                                                                                                                                      CGCTTACGCAGGCGGTTACTAGACTGGCCCCCTGAATCT‑CCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTT    <  1:419321/251‑1                                                                                                                                                                                                                                          TACGCAAGCGGTTACTAGACTGGCCCCCTGAATCT‑CCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTGTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCT  <  2:447646/249‑1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     CGCAGGAATGCGTAGATGCTGCATTGCACATAGCGGCAATGTTCAAATCGCTCGGCACCGGCAAGCTCGATATCCGTAATCAACAGAGCAACATCCGCCCGGCGGATATCCTCATCCGCTAAACGATTCTCCGTTCCCAGCGCGCCCTGAGTTTCAATGCTGACTCCCCACTTCTCTAACAGGCACAACTTTTCCAGCCGTTCCGCCGCCATATAAGTATGCGCCACGCCGCTTACGCAGGCGGTTACt‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4147403‑4147156 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TAGACTGGCCCCCTGAATCT‑CCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCT  >  NC_000913/4498181‑4498414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.