BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	364,237	Δ1,164 bp	coding (906‑2069/3075 nt)	lacZ ←	beta‑galactosidase

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	364237	365400	1164	65 [0]	[4] 69	lacZ	beta‑galactosidase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 364236	0 (0.000)	65 (1.200)	59/476	0.1	100%	coding (2070/3075 nt)	lacZ	beta‑galactosidase
*	?	NC_000913	365401 =	0 (0.000)	65 (1.200)	59/476	0.1	100%	coding (905/3075 nt)	lacZ	beta‑galactosidase

AGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACA‑GTTTC‑GGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTC‑ACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAAAGAAACTGTTACCCGTAGGTAGTCACGCAACTCGCCGCACATCTGAACTTCAGCCTCCAGTACAGCGCGGCTGAAATCATCATTAAAGCGAGTGGCAACATGGAAATCGCTGATTTGTGTAGT  >  NC_000913/365153‑365648

cgccaatccacctctgtgaaagaaagcctgactggcggttaaattgccaacgcttattacccagctcgatgcaaaaatccatttcgctggtggtcagatgcgggatggcgtgggacgcggcggggagcgtcacactgaggttttccgccagacgccactgctgccaggcgctgatgtgcccggcttctgaccatgcggtcgcgttcggttgcactacgcgtactgtgagccagagttgcccggcgctcca                                                                                                                                                                                                                                                           <  2:94368‑M2/2‑1 (MQ=255)

          catctgtgaaagaaagcctgactggcggttaaattgccaacgcttattacccagctcgatgcaaaaatccatttcgctggtggtcagatgcgggatggcgtgggacgcggcggggagcgtcacactgaggttttccgccagacgccactgctgccaggcgctgatgtgcccggcttctgaccatgcggtcgcgttcgggtgcactacgcgtactgtgagccagagttgcccggcgctcCACA‑GTTTC‑GGGt                                                                                                                                                                                                                                              <  1:355418‑M2/13‑1 (MQ=255)

                  aaagatagcctgactggcggttaaattgccaacgtttattgcccagcgcgatgcaaaaatccatgtcgctggttgtcagatgcgggatggcgtgggacgcggcggggagcgtcacactgaggttttccggtagatgcctctgctgccaggcgcttatgtgcccggcttctgaccatgcggtcgcgttcggttgcgctacgcgtactgtgagccagagttgcccggctctcCGCA‑GTTTC‑GGGTTTTCGACg                                                                                                                                                                                                                                      <  2:396555‑M2/21‑1 (MQ=255)

                              actggcggttaaactgccaacgcttattgcccagctcgatgcaaaaatccatttcgctggtggtcagatgctggatggcgtgggacgcggcggggagcgtcacactgaggttttccgccagacgccactgctgccaggcgctgatgtgcccggcttctgaccatgcggtcgcgttcggttgcactacgcgtactgtgagccagagttgcccggcgctcCACA‑GTTTC‑GGGTTTTCGACGTTCAGACGTAgt                                                                                                                                                                                                                          <  2:423753‑M2/33‑1 (MQ=255)

                               ctggcggttaaattgccaacgcttattacccagctcgatgcaaaaatccatttcgctggtggtcagatgcgggatggcgtgggacgcggcgggggacgtcacactgagggtttccgccagacgccactgctgccaggcgctgatgtgcccggcttctgaccatgcggtcgcggtcggttgcactacgcgtactgtgaggcagcggtgcccggcgctcCAAA‑GTTTCGGGGTTTTC‑ACGTTCCGACGtaggg                                                                                                                                                                                                                         >  1:224627‑M2/218‑249 (MQ=255)

                                    ggttaaattgccaacgcttattacccagctcgatgcaaaaatccatttcgctggtggtcagatgcgggatggcgtgggacgcggcggggaacgtcacacttaggttttccgccagacgccactgctgccaggcgctgatgtgcccggcttctgaccatgcgggcgcgttcggttgcaacacgcgaaccgtgagccagaggttcccgggcctcCACA‑GTTTc‑gggtttttgaccttccgagggggagtgccg                                                                                                                                                                                                                    >  2:310722‑M2/213‑236 (MQ=255)

                                            tgccaacgcttattacccagctcgatgcaaaaatccatttcgctggtggtcagatgcgggatggcgtgggacgcggcggggagcgtcaccctggggttttccgccagacgccactgctgccaggcgctgatgtggccggcttctggccctgcggtcgcgttcggttgcactacgcgtactgtgagccagagttgcccggcgctcCCCA‑GTTTC‑GGGTTTTCGACGTTCAGACGTAGTGTGACGCGCTCGGc                                                                                                                                                                                                            >  2:423070‑M2/205‑251 (MQ=255)

                                             gccaacgcttattacccagctcgatgcaaaaatccatttcgctggtggtcagatgcgggatggcgtgggacgcggcggggagcgtcacactgaggttttccgccagacgccactgctgccaggcgctgatgtgcccggcttctgaccatgcggtcgcgttcggttgcactacgcgtactgtgagccagagttgcccggcgctcCACA‑GTTTC‑GGGTTTTCGACGTTCAGACGTAGTGGGACGCGATCGGc                                                                                                                                                                                                            >  1:32800‑M2/204‑250 (MQ=255)

                                              ccaacgcttcttacccagatcgctgcaaaaatccctttcgctggtggtcagatgcgggatggcgtgggacgcggcggggagcgtcacactgaggttttccgccagacgccactgctgccaggagctgatgtgcccggcttctgaccatgcggtcgcgttcggttgcactacgcgtactgtgagcccgagttgcccggcgctcCCCC‑GTTTC‑GGGGTTTCGACGTTCAGACGTTGTGTGGCGCGATCGgcc                                                                                                                                                                                                           >  1:174802‑M2/203‑249 (MQ=255)

                                              ccaacgcttattacccagctcgatgcaaaaatccatttcgctggtggtcagatgcgggatggcgtgggacgcggcggggagcgtcacactgaggttttccgccagacgccactgctgccaggcgctgatgtgcccggcttctgaccatgcggtcgcgttcggttgcactacgcgtactgtgagccagagttgcccggcgctcCACA‑GTTTC‑GGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCAt                                                                                                                                                                                                          <  1:241838‑M2/49‑1 (MQ=255)

                                                   gcttattacccagctcgatgcaaaaatccatttcgctggtggtcagatgcgggatggcgtgggacgcggcggggagcgtcacactggggttttccgccagacgccactgctgccaggcgctgatgtgcccggcttctgaccatgcggtcgcgttcggttgcactacgcgtactgtgagccagaggtgccccgcgctcCACA‑GTTTC‑GGGTTTTCGACGTTCCGACGTAGGGTGACGCGATCGGCCTaaccc                                                                                                                                                                                                     >  2:470462‑M2/198‑250 (MQ=255)

                                                      tattacccagctcgatgcaaaaatccatttcgctggtggtcagatgcgggatggcgtgggacgcggcggggagcgtcacactgaggttttccgccagacgccactgctgccaggcgctgatgtgcccggcttctgaccatgcggtcgcgttcggttgcactacgcgtactgtgagccagagttgcccggcgctcCACA‑GTTTC‑GGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAaccacc                                                                                                                                                                                                   <  1:423070‑M2/56‑1 (MQ=255)

                                                         tacccagctcgatgcaaaaatccatttcgctggtggtcagatgcgggatggcgtgggacgcggcggggagcgtcacactgaggttttccgccagacgctactgctgccaggcgctgatgtgcccggcttctgaccatgcggtcgcgtgcggttgcactacgcgtactgtgagccagagttgcccggcgctcCACA‑GTTTC‑GGGTGTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACg                                                                                                                                                                                                <  1:239959‑M2/59‑1 (MQ=255)

                                                           cccagctcgatgcaaaaatccatttcgctggtggtcagatgcgggatggcgtgggacgcggcggggagcgtcacactgaggttttccgccagacgccactgctgccaggcgctgatgtgcccggcttctgaccatgcggtcgcgttcggttgcactacgcgtactgtgagccagagttgcccggcgctcCACA‑GTTTC‑GGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTc                                                                                                                                                                                             <  1:443510‑M2/62‑1 (MQ=255)

                                                              agctcgatgcaacaaaccagttcgctggtggtcagatgggggagggcgtgggacgcggcggggagcttcacactgaggttgtccgccagacgccactgctgccaggcgctgatgtgcccggcttctgaccatgcggtcgcgttcggttgcactacgcgtactgtgagccagagttgcccggcgctcCACA‑GTTTT‑GGGTTTTCGACGTTCAGACGTAGTGTGACACGATCGGCATAACCACCACGCTCATc                                                                                                                                                                                          <  2:62037‑M2/65‑1 (MQ=255)

                                                               gctcgatgcaaaaatccatttcgctggtggtcagatgcgggatggcgtgggacgcggcggggagcgtcacactgaggttttccgccagacgccactgctgccaggcgctgatgtgcccggcttctgaccatgcggtcgcgttcggttgcactacgcgtactgtgagccagagttgcccggcgctcCACA‑GTTTC‑GGGTTTTCGACGGTCAGACGTAGTGTGACGCGATCGGCATAACCACCCCGCTCATCg                                                                                                                                                                                         >  1:425482‑M2/186‑251 (MQ=255)

                                                                       caaaaatccatttcgctgggggtcagatgcgggatggcgtgggacgcggcggggagcgtcacactgaggttttccgcctgacgccactgctgccaggcgctgatgtgcccggcttctgaccatgcggtcgcgttcggttgctctacgcgtgctgtgagccagagttgcccggcgctcCACA‑GGTTC‑GGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTc                                                                                                                                                                                 <  1:548257‑M2/74‑1 (MQ=255)

                                                                        aaaaatccatttcgctggtggtcagatgcgggatggcgtgggacgcggcggggagcgtcacacttaggttttccgacagacgccactgctgccaggcgctgatgtgcccggcttctggccatgaggtcgcgttcggttgcactacgcgtactgtgagccagagttgcccggcgctcCCCA‑GTTTC‑GGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCCCCTCGATAATTtcc                                                                                                                                                                                >  1:232544‑M2/177‑250 (MQ=255)

                                                                           aatccatttcgctggtggtcagatgcgggatggcgtgggccgcggcggcgagagtcacaccgaggatttccggcagacgcccctgctgcccggcgctcatgtgcccggcttctcaccctgcggtcgcgttcggttgcactacgcgtactgtgagccagagttgcccggcgctcCACA‑GTTTC‑GGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAAAttc‑aacc                                                                                                                                                                            >  2:42341‑M2/174‑248 (MQ=255)

                                                                           aatccatttcgctggtggtcagatgcgggatggcgtgggacgcggccgggagcgtcacactgagggtttccgccagacgccactgctgccaggcgctgttgtgcccggctcccgaccatgcggtcgcgttcggttgcactacgcgtactgtgagcccgagttgcccggcgctcCACA‑GTTTC‑GGGGTTTCGACGTTCAGGAGTAGGGGGACGCGATCGGCATAACCACCACGCTCATCGATAATTTC‑Accg                                                                                                                                                                            >  1:485944‑M2/174‑251 (MQ=255)

                                                                            atccatttcgctggtggtcagatgcgggatggcgtgggacgcggcggggagcgtcacactgaggttttccgccagacgccactgctgccaggcgctgatgtgcccggcttctgaccatgcggtcgcgttcggttgcactacgcgtactgtgagccagagttgcccggcgctcCACA‑GTTTC‑GGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTC‑Accgc                                                                                                                                                                           <  1:309254‑M2/79‑1 (MQ=255)

                                                                                   tcgctggtggtcagatgcgggatggcgtgggacgcggcggggagcgtcacactgaggttttccgccagacgccactgctgccaggcgctgatgtgcccggcttctgaccatgcggtcgcgttcggttgcactacgcgtactgtgagccagagttgcccggcgctcCACA‑GTTTC‑GGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTC‑ACCGCCGAAAgg                                                                                                                                                                    >  1:176708‑M2/166‑251 (MQ=255)

                                                                                   tcgcgggtggtcagatgcgggatggcgtgggacgcggcggggagcgtcacactgaggttttccgccagacgccactgctgccaggcgcggatgtgcccggcttttgaccaggcggtcgcgtgcggttgcactacgcttcctgtgagccagagttgcccggcgctcCACA‑GTTTC‑GGGTTTTCGACTTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCACCGATAATGTC‑GCCGCCGAAAg                                                                                                                                                                     <  2:514261‑M2/85‑1 (MQ=255)

                                                                                    cgctggtggtcagatgcgggatggcgtgggacgcggcggggagcgtcacactgaggttttccgccagacgccactgctgcccggcgctgatgtgcccggcttctgacccagcggtcgcgttcggttgccctacgcgtactgtgagccagagattccaggcgctcCACA‑GATTC‑CGGGTTTCGACGTTCCGACGTAGAGGGACGCGATCGACATAACCACCCAGCTCATCGATAATTTA‑ACCCCCGAAAGgc                                                                                                                                                                   >  1:562736‑M2/165‑251 (MQ=255)

                                                                                        gggggtcagatgcgggatggcgtgggacgcggcggggagcgtcacacggaggttttccgccagacgccactgctgccaggcgctgatgtgcccggcttctgaccatgcggtcgcgttcggttgcactacgcgtactgtgagccagagttgcccggcgctcCACA‑GTTTC‑GGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTC‑ACCGCCGAAAGGcgcg                                                                                                                                                                <  1:346464‑M2/90‑1 (MQ=255)

                                                                                              cagatgcgggatggcgtgggacgcggcggggagcgtcacactgcggttttccgccagacgccactgctgccaggcgctgatgtgcccggcttctgaccatgcggtcgcgttcggttgcactacgcgtactgtgagccagagttgcccggcgctcCACA‑GTTTC‑GGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTC‑ACCGCCGAAAGGCGCGGTGCCGc                                                                                                                                                         >  1:504131‑M2/155‑251 (MQ=255)

                                                                                                  ggcgggatggcgtgggccgcggcggggagcgtcaccctgaggttttccgccagacgccactgctgccaggcgctgatgtgcccggcttctgaccatgcggtcgcgttcggttgcactacgcgtactgtgagccagagttgcccggcgctcCACA‑GTTTC‑GGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTC‑ACCGCCGAAAGGCGCGGTGCCGCTGGc                                                                                                                                                     <  1:291792‑M2/101‑1 (MQ=255)

                                                                                                   gcgggatgggggggggcgcggcggggggcgtcacactgaggttttccgccagacgccactgctgccaggcgctgatgtgcccggcttctgcccatgcggtcgcgttcggttgcactacgcgtactgtgagccagagttgcccggcgctcCACA‑TTTTC‑GGGTTTTCGACGTTCAGACGAAGTGTTACGCGATCGGCATAACCACCACGCTCATCGATAATTTC‑ACCGCCGAAAGGCGCGGTGCCGCTGGc                                                                                                                                                     <  1:507619‑M2/101‑1 (MQ=255)

                                                                                                    cgggatggagtgggacgcggcggggagcgtcacactgaggttttccgccagacgccactgctgccaggcgctgatgtgcccggcttctgaccatgcggtcgcgttcggttgcactacgcgtactgtgagccagagttgcccggcgctcCACA‑GTTTC‑GGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAAACACCACGCTCTTCGATAATTTC‑CCCGCCCAAAGGCGCGGTGCCGCTGGCg                                                                                                                                                    >  1:100451‑M2/149‑250 (MQ=255)

                                                                                                          ggcgtgggacgcggcggggggcgtcacccggaggttttccgccagacgccgctgctgccgggcgctgatgtgcccggcttctgaccatgcggtcgcgttcggttgcactacgcgtactgggagccagagtggcccggcgctcCACA‑GTTTC‑GGGTTTTCGACTTTCAGACGTAGTGTGACGCGATCGGCATAACCCCCACGCTCATCGATAATTTC‑ACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGc                                                                                                                                              <  2:453820‑M2/108‑1 (MQ=255)

                                                                                                             gtgggacgcggcggggagcgtccccctggggttttccgccagacgccactgctgccaggcgctgatgtgcccggcttctgaccatgcggtcgcgttcggttgccctacgcgtactgtgagccagagttgcccggcgctcCACA‑GTTTC‑GGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCCCGCTCATCGATAATTTC‑ACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGttt                                                                                                                                          <  1:1953‑M2/112‑1 (MQ=255)

                                                                                                             gtgggacgcggcggggagcgtcccactgaggtttcccgccagacgccactgctgcaaggcgctgatgtgcccggcttctgcccatgcggtcgcgttcggttgcactacgcgtactgtgagcaagagttgcccggcgctcCACA‑TTTTC‑GGGTTTTCGACGTTCAGACGTAGTTGGACGCGATCGGCATAACCACCACGCTCATCGATAATTTC‑ACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGttt                                                                                                                                          <  1:261245‑M2/112‑1 (MQ=255)

                                                                                                             gtgggacgcggcggggagcgtcacactgaggttttccgccagacgccactgctgccaggcgctgatgtgcccggcttctgaccatgcggtcgcgttcggttgcactacgcgtactgtgagccagagttgcccggcgctcCACA‑GTTTC‑GGGTTTTCGACGTTCAGACGTAGTGTGACGCGATc                                                                                                                                                                                                               <  2:195001‑M2/44‑1 (MQ=255)

                                                                                                             gtgggacgcggcggggagcgtcacactgaggttttccgccagacgccactgctgccaggcgctgatgtgcccggcttctgaccatgcggtcgcgttcggttgcactacgcgtactgtgagccagagttgcccggcgctcCACA‑GTTTC‑GGGGTTTCGACGTTCAGACGTAGTGTGACGCGATc                                                                                                                                                                                                               >  1:195001‑M2/140‑183 (MQ=255)

                                                                                                               gggacgcggcggggagcgtcccactgaggttttccgccagacgccactgctgccaggcgctgatgtgcccggcttctgaccatgcggtcgcgttcggttgcactacgcgtactgtgagccagagttgcccggcgctcCACA‑GTTTC‑GGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTC‑ACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTc                                                                                                                                         >  1:413465‑M2/138‑250 (MQ=255)

                                                                                                                         ggggagcgtcacactgaggttttccgccagacgccactgctgccaggcgctgatgtgcccggcttctgaccatgcggtcgcgttcggttgcactacgcgtactgtgagccagagttgcccggcgctcCACA‑GTTTC‑GGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTC‑ACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCAt                                                                                                                               >  2:89888‑M2/128‑250 (MQ=255)

                                                                                                                          gggagcgtcacactgaggttttccgccagacgccactgctgccaggcgctgatgtgcccggcttctgaccatgcggtcgcgttcggttgcactacgcgtactgtgagccagagttgcccggcgctcCACA‑GTTTC‑GGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCCCCACGCTCATCGATAATTTC‑ACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATaa                                                                                                                             <  1:63865‑M2/125‑1 (MQ=255)

                                                                                                                          gggagcgtcacactgaggttttccgccagacgccactgctgccaggcgctgatgtgcccggcttctgaccatgcggtcgcgttcggttgcactacgcgtactgtgagccagagttgcccggcgctcCACA‑GTTTC‑GGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGCTAATTTC‑ACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATa                                                                                                                              >  2:446056‑M2/127‑250 (MQ=255)

                                                                                                                           ggagcgtcacactgaggttttccgccagacgccactgctgccaggcgctgatgtgcccggcttctgaccatgcggtcgcgttcggttgcactacgcgtactgtgagccagagttgcccggcgctcCACA‑GTTTC‑GGGTTTTCGACGTTCAGCCGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTC‑ACCGCCGAACGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATaa                                                                                                                             >  2:504543‑M2/126‑250 (MQ=255)

                                                                                                                                gtcacactgaggttttccgccagacgccactgctgccaggcgctgatgtgcccggcttctgacaatgcggtcgcgttcggttgcactacgcgtactgtgagccagagttgcccggcgctcCACA‑GTTTC‑GGGTTTTCGACGTTCAGACGTAGGGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTC‑ACCGCCGAAAGGCGCGGGGCCGCTGGCGACCTGCGTTTCACCCTGCCATAAAGAAAc                                                                                                                       >  2:207206‑M2/121‑251 (MQ=255)

                                                                                                                                 tcacactgaggttttccgccagacgccactgctgccaggcgctgatgtgcccggcttctgaccatgcggtcgcgttcggttgcactacgcgtactgtgagccagagttgcccggcgctcCACA‑GTTTC‑GGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTC‑ACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAAAGAAACt                                                                                                                      >  1:7778‑M2/120‑251 (MQ=255)

                                                                                                                                     actgaggttttccgccagacgccactgctgccaggcgctgatgtgcccggcttctgaccatgcggtcgcgttcggttgcactacgcgtactgtgagccagagttgcccggcgctcCACA‑GTTTC‑GGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTC‑ACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAAAGAAACTGtt                                                                                                                   >  2:8063‑M2/116‑250 (MQ=255)

                                                                                                                                        gaggttttccgccagacgccactgctgccaggcgctgatgtgcccggcttctgaccatgcggtcgcgttcggttgcactacgcgtactgtgagccagagttgcccggcgctcCACA‑GTTTC‑GGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTC‑ACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAAAGAAACTGTTAccc                                                                                                               >  1:40580‑M2/113‑251 (MQ=255)

                                                                                                                                                          ccactgctgccaggcgctgatgtgcccggcttctgaccatgcggtcgcgttcggttgcactacgcgtactgtgagccagagttgcccggcgctcCACA‑GTTTC‑GGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTC‑ACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAAAGAAACTGTTACCCGTAGGTAGTCACGCAACt                                                                                             >  1:425293‑M2/95‑251 (MQ=255)

                                                                                                                                                           cactgctgccaggcgctgatgtgcccggcttctgaccatgcggtcgcgttcggttgcactacgcgtactgtgagccagagttgcccggcgctcCACA‑GTTTC‑GGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTC‑ACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAAAGAAACTGTTACCCGTAGGTAGTCACGCAACTc                                                                                            >  1:10678‑M2/94‑251 (MQ=255)

                                                                                                                                                           cactgctgccaggcgctgatgtgcccggcttctgaccatgcggtcgcgttcggttgcactacgcgtactgtgagccagagttgcccggcgctcCACA‑GTTTC‑GGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTC‑ACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAAAGAAACTGTTACCCGTAGGTAGTCACGCAACTc                                                                                            >  1:60717‑M2/94‑251 (MQ=255)

                                                                                                                                                           cactgctgccaggcgctgatgtgcccggcttctgaccatgcggtcgcgttcggttgcactacgcgtactgtgagccagagttgaccggcgctcCACA‑GTTTC‑GGGTTTTCGACGTTCAGACGTAGAGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTC‑ACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAAAGAAACTGTTACCCGTAGGTAGTCACGCAACTc                                                                                            >  1:566384‑M2/94‑251 (MQ=255)

                                                                                                                                                                     aggcgctgatgtgcccggcttctgaccatgcggtcgcgttcggttgcactacgcgtactgtgagccagagttgcccggcgctcCACA‑GTTTC‑GGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTC‑ACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAAAGAAACTGTTACCCGTAGGTAGTCACGCAACTCGCCGCACATc                                                                                  >  1:348‑M2/84‑251 (MQ=255)

                                                                                                                                                                        cgctgatgtgcccggcttctgaccatgcggtcgcgttcggttgcactacgcgtactgtgagccagagttgcccggcgctcCACA‑GTTTC‑GGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTC‑ACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAAAGAAACTGTTACCCGTAGGTAGTCACGCAACTCGCCGCACATCTg                                                                                >  1:190532‑M2/81‑250 (MQ=255)

                                                                                                                                                                         gctgatgtgcccggcttctgaccatgcggtcgcgttcggttgcactacgcgtactgtgagccagagttgcccggcgctcCACA‑GTTTC‑GGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCCATAATTTC‑ACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAAAGAAACTGTTACCCGTAGGTAGTCACGCAACTCGCCGCACATCTGa                                                                               >  1:27226‑M2/80‑250 (MQ=255)

                                                                                                                                                                                         tctggccatgcgtgcgggttcggttgcactacgcgtactgtgagccagagttgcccggcgcccCCCA‑GTTTC‑GGGTTTTCGACGTTCAGAGGTAGTGTGACGCGATCGGCATAACCACCACGCTCAGCGATAATTTC‑ACCGCCGAAAGGCGCGGTGCCGCTGTCGACCTGCGTTTCACCCTGCCATAAAGAAACTGTTACCCGTAGGTAGTCACGCAACTCGCCGCACATCTGAACTTCAGCCTCCAGt                                                                <  1:246418‑M2/186‑1 (MQ=255)

                                                                                                                                                                                            aaccatgcggtcgcgttcggttgcactacgcgtactgtgagccagagttgcccggggctcCACA‑GTTTC‑GGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCCCGCTCATCGATAATTTC‑ACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAAAGAAACTGTTACCCGTAGGTAGTCACGCAACTCGCCGCACATCTGAACTTCAGCCTCCAGTACAgc                                                           <  1:399417‑M2/191‑1 (MQ=255)

                                                                                                                                                                                             accagggggtcgcgttcggttgcattaggcgtactgggagccagagtggcccggcgctcCACA‑GTTTC‑GGGTTTTCGACGTTCAGCCGTGGTGGGACGCGATCGGCATAACCACCCCGCTCATCGATAATTTC‑ACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAAAGAAACTGTTACCCGTAGGTAGTCACGCAACTCGCCGCACATCTGAACTTCAGCCTCCAGTACAgcg                                                          <  2:2432‑M2/192‑1 (MQ=255)

                                                                                                                                                                                                   cggtcgcgttcgggtgcactacgcgtactgtgagcccgagctgcccggcgctaCACA‑GTTTC‑GGGTTTTCGACGTCCAGACGTAGTGTAACCCGATCGGCATAACCACCCCGCTCATCGATAATTTC‑ACCGCCGAAAGGCGCGGCGCCGCTGGCGACCTGCGTCTCACCCTGCCATAAAGCAACTGTTCCCAGTAGGTAGTCACGCAACACGCCGCACATCTGCACTTCAGCCTCCAGTAAAGcgcggatg                                                    >  2:47912‑M2/54‑248 (MQ=255)

                                                                                                                                                                                                          gttcggttgcactacgcgtactgtgagccagagttgcccggcgctcCACA‑GTTTC‑GGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTC‑ACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAAAGAAACTGTTACCCg                                                                                                              >  1:310046‑M2/47‑186 (MQ=255)

                                                                                                                                                                                                              ggttgcactacgcgtactgtgagccagagttgcccggcgctcCACA‑TTTTC‑GGTTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTC‑ACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAAAGAAACTGTTACCCGTAGGTAGTCACGCAACTCGCCGCACATCTGAACTTCAGCCTCCAGTACAGCGCGGCTGAAATCATCAtt                                         <  1:164269‑M2/209‑1 (MQ=255)

                                                                                                                                                                                                                  gcactacgcgtactgtgagccagagttgcccggcgctcCACA‑GTTTC‑GGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTC‑ACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAAAGAAACTGTTACCCGTAGGTAGTCACGCa                                                                                                >  2:154687‑M2/39‑192 (MQ=255)

                                                                                                                                                                                                                  gcactacgcgtactgtgagccagagttgcccggcgctcCACA‑GTTTC‑GGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTC‑ACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAAAGAAACTGTTACCCGTAGGTAGTCACGCa                                                                                                <  1:154687‑M2/154‑1 (MQ=255)

                                                                                                                                                                                                                    actacgcgtactgtgagccagagttgcccggcgctcCACA‑GTTTC‑GGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTC‑ACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAAAGAAACTGTTACCCGTAGGTAGTCACGCAACTCGCCGCACATCTGAACTTCAGCCTCCAGTACAGCGCGGCTGAAATCATCATTAAAGCg                                   >  1:338089‑M2/37‑251 (MQ=255)

                                                                                                                                                                                                                        cgcgtactgtgagccagaggtgcccggcgctcCACA‑GTTTC‑GGGTTTTCGGCGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTC‑ACCGCCGACAGGCGCGGTACCGCTGGCGACCTGCGTTTCACCCTGCCATAAAGAAACTGTTACCCGTAGGTAGTCACGCAACTCGCCGCACATCTGAAATTCAGCCTCCAGTACAGCGCGGCTGAAATCATACTTAAAGCGAGTg                               >  2:298983‑M2/33‑251 (MQ=255)

                                                                                                                                                                                                                                   ggccagagttgcccggcgctcCACA‑GTTGC‑GGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCCCCACGCTCATCGATAATTTC‑ACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAAAGAAACTGTTACCCGTAGGTAGTCACGCAACTCGCCGCACATCTGAACTTCAGCCTCCAGTACAGCGCGGCTGAAATCATCATTAAAGCGAGTGGCAACATGGaa                    <  1:169494‑M2/230‑1 (MQ=255)

                                                                                                                                                                                                                                   gcccagagttgcccgccgctcCACA‑‑TGTCGGGGTTTTCGACGTCCAGACGTGGTGTGACCCGATCGGAATAACCACCCCGCCCATCGAAAATTTC‑CCCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAAAGAAACTGTTACCCGTAGGTAGTCACGCAACTCGCCGCACATCTGAACTTCAGCCTCCAGTACAGCGCGGCTGAAATCATCATTAAAGCGAGTGGCAACATGGaa                    <  1:24065‑M2/230‑1 (MQ=255)

                                                                                                                                                                                                                                    gccagagttgccgggccctcCACA‑GTGTC‑GGTTTTCCGACGTTCAGACGGAGTGTGACGCGATCGGAATAACCACCACGCTCACCGATAATTTC‑CCCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAAAGAAACTGTTACCCGTAGGTAGTCACGCAACTCGCCGCACATCTGAACTTCAGCCTCCAGTACAGCGCGGCTGAAATCATCATTAAAGCGAGTGGCAACATGGaaa                   <  2:144425‑M2/231‑1 (MQ=255)

                                                                                                                                                                                                                                       ggagtggcccggcgctcAACA‑GTTTG‑GGGTTTTGGACGTTCAGCCGTGGTGGGACGCGACCGGCATAACCACCACGCTCATCGATATTTTC‑ACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAAAGAAACTGTTACCCGTAGGTAGTCACGCAACTCGCCGCACATCTGAACTTCAGCCTCCAGTACAGCGCGGCTGAAATCATCATTAAAGCGAGTGGCAACATGGAAATCg                <  2:217740‑M2/234‑1 (MQ=255)

                                                                                                                                                                                                                                        gagttgcccggcgctcCACA‑GTTTC‑GGGTTTTCGCCGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTC‑ACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAAAGAAACTGTTACCCGTAGGTAGTCACGCAACTCGCCGCACATCTGAACTTCAGCCTCCCGTACAGCGCGGCTGACATCCTCATTAAAGCGAGTGGCAACATGGAAATCGc               >  2:59724‑M2/17‑251 (MQ=255)

                                                                                                                                                                                                                                          gttgcccGGCGCTCC‑CATGTTGC‑GGGTTTTCGCGGTTCAGACGTAGTGTGACGCTGTCGGCTTACCTCCCCCGCCCATCTA‑AATTTCCGCCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTGTCACCCTGCCATAAAGAAACTGTTACCCGTAGGTAGTCACGCAACTCGCCGCACATCTGAACGTCAGCCTCCAGTTCAGCGCGGCGGAAATCAGCATTTAAGCGAGTGGCAACATGGAAATCGCTg             <  2:485944/245‑1 (MQ=255)

                                                                                                                                                                                                                                                  gcgcCCCACA‑GTTTC‑GGGTTTTCGACGTCCAGCCGTAGTGTGACGCGAGCGGCATACCCCCCCCGCTCACCGAAAATTCC‑CCCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAAAGAAACTGTTACCCGTAGGTAGTCACGCAACTCGCCGCACATCTGAACTTCAGCCTCCAGTACAGCGCGGCTGAAATCATCATTAAAGCGAGTGGCAACATGGAAATCGCTGATTTgtgt     <  2:40580/251‑1 (MQ=255)

                                                                                                                                                                                                                                                  gcgcCCCACA‑GTTGC‑GGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGAATACCCACCACGCTCATCAATAATTTC‑ACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAAAGAAACTGTTACCCGTAGGTAGTCACGCAACTCGCCGCACATCTGAACTTCAGCCTCCAGTACAGCGCGGCTGAAATCATCATTAAAGCGAGTGGCAACATGGAAATCGCTGATTTgtgt     <  1:8063/251‑1 (MQ=255)

                                                                                                                                                                                                                                                     cTCCACA‑GTTTC‑GGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTC‑ACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCCCCCTGCCATCAAGAAACTGTTACCCGTAGGTAGTCACGCAACTCGCCGCACATCTGAACTTCAGCCTCCAGTACAGCGCGGCTGAAATCATCATTAAAGCGAGTGGCAACATGGAAATCGCTGATTTGTGTAGt  >  2:481401/1‑251 (MQ=255)

AGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACA‑GTTTC‑GGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTC‑ACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAAAGAAACTGTTACCCGTAGGTAGTCACGCAACTCGCCGCACATCTGAACTTCAGCCTCCAGTACAGCGCGGCTGAAATCATCATTAAAGCGAGTGGCAACATGGAAATCGCTGATTTGTGTAGT  >  NC_000913/365153‑365648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`