Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	364,237	Δ1,164 bp	coding (906‑2069/3075 nt)	lacZ ←	beta‑galactosidase

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	364237	365400	1164	65 [0]	[4] 69	lacZ	beta‑galactosidase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 364236	0 (0.000)	65 (1.200)	59/476	0.1	100%	coding (2070/3075 nt)	lacZ	beta‑galactosidase
	?	NC_000913	365401 =	0 (0.000)					coding (905/3075 nt)	lacZ	beta‑galactosidase

CGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACTGAGGTTTTCCGCCAGACGCCACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTGACCATGCGGTCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGGTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGC  >  NC_000913/363989‑364471                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                            cGCCAATCCACCTCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACTGAGGTTTTCCGCCAGACGCCACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTGACCATGCGGTCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCca                                                                                                                                                                                                                                           <  2:94368‑M1/250‑3 (MQ=255)           caTCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACTGAGGTTTTCCGCCAGACGCCACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTGACCATGCGGTCGCGTTCGGGTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCcacagtttcgggt                                                                                                                                                                                                                                <  1:355418‑M1/251‑14 (MQ=255)                   aaagatagCCTGACTGGCGGTTAAATTGCCAACGTTTATTGCCCAGCGCGATGCAAAAATCCATGTCGCTGGTTGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACTGAGGTTTTCCGGTAGATGCCTCTGCTGCCAGGCGCTTATGTGCCCGGCTTCTGACCATGCGGTCGCGTTCGGTTGCGCTACGCGTACTGTGAGCCAGAGTTGCCCGGCTCTCcgcagtttcgggttttcgacg                                                                                                                                                                                                                        <  2:396555‑M1/251‑22 (MQ=255)                               aCTGGCGGTTAAACTGCCAACGCTTATTGCCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCTGGATGGCGTGGGACGCGGCGGGGAGCGTCACACTGAGGTTTTCCGCCAGACGCCACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTGACCATGCGGTCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCcacagtttcgggttttcgacgttcagacgtagt                                                                                                                                                                                                            <  2:423753‑M1/251‑34 (MQ=255)                                cTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGGACGTCACACTGAGGGTTTCCGCCAGACGCCACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTGACCATGCGGTCGCGGTCGGTTGCACTACGCGTACTGTGAGGCAGCGGTGCCCGGCGCTCcaaagtttcggggttttcacgttccgacgtaggg                                                                                                                                                                                                           >  1:224627‑M1/1‑217 (MQ=255)                                     ggTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAACGTCACACTTAGGTTTTCCGCCAGACGCCACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTGACCATGCGGGCGCGTTCGGTTGCAACACGCGAACCGTGAGCCAGAGGTTCCCGGGCCTCcacagtttcgggtttttgaccttccgagggggagtgccg                                                                                                                                                                                                      >  2:310722‑M1/1‑212 (MQ=255)                                             tGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACCCTGGGGTTTTCCGCCAGACGCCACTGCTGCCAGGCGCTGATGTGGCCGGCTTCTGGCCCTGCGGTCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCcccagtttcgggttttcgacgttcagacgtagtgtgacgcgctcggc                                                                                                                                                                                              >  2:423070‑M1/1‑204 (MQ=255)                                              gCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACTGAGGTTTTCCGCCAGACGCCACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTGACCATGCGGTCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCcacagtttcgggttttcgacgttcagacgtagtgggacgcgatcggc                                                                                                                                                                                              >  1:32800‑M1/1‑203 (MQ=255)                                               ccAACGCTTCTTACCCAGATCGCTGCAAAAATCCCTTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACTGAGGTTTTCCGCCAGACGCCACTGCTGCCAGGAGCTGATGTGCCCGGCTTCTGACCATGCGGTCGCGTTCGGTTGCACTACGCGTACTGTGAGCCCGAGTTGCCCGGCGCTCccccgtttcggggtttcgacgttcagacgttgtgtggcgcgatcggcc                                                                                                                                                                                             >  1:174802‑M1/1‑202 (MQ=255)                                               ccAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACTGAGGTTTTCCGCCAGACGCCACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTGACCATGCGGTCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCcacagtttcgggttttcgacgttcagacgtagtgtgacgcgatcggcat                                                                                                                                                                                            <  1:241838‑M1/251‑50 (MQ=255)                                                    gCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACTGGGGTTTTCCGCCAGACGCCACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTGACCATGCGGTCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGGTGCCCCGCGCTCcacagtttcgggttttcgacgttccgacgtagggtgacgcgatcggcctaaccc                                                                                                                                                                                       >  2:470462‑M1/1‑197 (MQ=255)                                                       tattaCCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACTGAGGTTTTCCGCCAGACGCCACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTGACCATGCGGTCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCcacagtttcgggttttcgacgttcagacgtagtgtgacgcgatcggcataaccacc                                                                                                                                                                                     <  1:423070‑M1/250‑57 (MQ=255)                                                          taCCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACTGAGGTTTTCCGCCAGACGCTACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTGACCATGCGGTCGCGTGCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCcacagtttcgggtgttcgacgttcagacgtagtgtgacgcgatcggcataaccaccacg                                                                                                                                                                                  <  1:239959‑M1/250‑60 (MQ=255)                                                            cccAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACTGAGGTTTTCCGCCAGACGCCACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTGACCATGCGGTCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCcacagtttcgggttttcgacgttcagacgtagtgtgacgcgatcggcataaccaccacgctc                                                                                                                                                                               <  1:443510‑M1/251‑63 (MQ=255)                                                               aGCTCGATGCAACAAACCAGTTCGCTGGTGGTCAGATGGGGGAGGGCGTGGGACGCGGCGGGGAGCTTCACACTGAGGTTGTCCGCCAGACGCCACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTGACCATGCGGTCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCcacagttttgggttttcgacgttcagacgtagtgtgacacgatcggcataaccaccacgctcatc                                                                                                                                                                            <  2:62037‑M1/251‑66 (MQ=255)                                                                gCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACTGAGGTTTTCCGCCAGACGCCACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTGACCATGCGGTCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCcacagtttcgggttttcgacggtcagacgtagtgtgacgcgatcggcataaccaccccgctcatcg                                                                                                                                                                           >  1:425482‑M1/1‑185 (MQ=255)                                                                        cAAAAATCCATTTCGCTGGGGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACTGAGGTTTTCCGCCTGACGCCACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTGACCATGCGGTCGCGTTCGGTTGCTCTACGCGTGCTGTGAGCCAGAGTTGCCCGGCGCTCcacaggttcgggttttcgacgttcagacgtagtgtgacgcgatcggcataaccaccacgctcatcgataatttc                                                                                                                                                                   <  1:548257‑M1/251‑75 (MQ=255)                                                                         aaaaaTCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACTTAGGTTTTCCGACAGACGCCACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTGGCCATGAGGTCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCcccagtttcgggttttcgacgttcagacgtagtgtgacgcgatcggcataaccaccacgcccctcgataatttcc                                                                                                                                                                  >  1:232544‑M1/1‑176 (MQ=255)                                                                            aaTCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGCCGCGGCGGCGAGAGTCACACCGAGGATTTCCGGCAGACGCCCCTGCTGCCCGGCGCTCATGTGCCCGGCTTCTCACCCTGCGGTCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCcacagtttcgggttttcgacgttcagacgtagtgtgacgcgatcggcataaccaccacgctcatcgataaattcaacc                                                                                                                                                               >  2:42341‑M1/1‑173 (MQ=255)                                                                            aaTCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCCGGGAGCGTCACACTGAGGGTTTCCGCCAGACGCCACTGCTGCCAGGCGCTGTTGTGCCCGGCTCCCGACCATGCGGTCGCGTTCGGTTGCACTACGCGTACTGTGAGCCCGAGTTGCCCGGCGCTCcacagtttcggggtttcgacgttcaggagtagggggacgcgatcggcataaccaccacgctcatcgataatttcaccg                                                                                                                                                               >  1:485944‑M1/1‑173 (MQ=255)                                                                             aTCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACTGAGGTTTTCCGCCAGACGCCACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTGACCATGCGGTCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCcacagtttcgggttttcgacgttcagacgtagtgtgacgcgatcggcataaccaccacgctcatcgataatttcaccgc                                                                                                                                                              <  1:309254‑M1/251‑80 (MQ=255)                                                                                    tCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACTGAGGTTTTCCGCCAGACGCCACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTGACCATGCGGTCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCcacagtttcgggttttcgacgttcagacgtagtgtgacgcgatcggcataaccaccacgctcatcgataatttcaccgccgaaagg                                                                                                                                                       >  1:176708‑M1/1‑165 (MQ=255)                                                                                    tCGCGGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACTGAGGTTTTCCGCCAGACGCCACTGCTGCCAGGCGCGGATGTGCCCGGCTTTTGACCAGGCGGTCGCGTGCGGTTGCACTACGCTTCCTGTGAGCCAGAGTTGCCCGGCGCTCcacagtttcgggttttcgactttcagacgtagtgtgacgcgatcggcataaccaccacgctcaccgataatgtcgccgccgaaag                                                                                                                                                        <  2:514261‑M1/250‑86 (MQ=255)                                                                                     cGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACTGAGGTTTTCCGCCAGACGCCACTGCTGCCCGGCGCTGATGTGCCCGGCTTCTGACCCAGCGGTCGCGTTCGGTTGCCCTACGCGTACTGTGAGCCAGAGATTCCAGGCGCTCcacagattccgggtttcgacgttccgacgtagagggacgcgatcgacataaccacccagctcatcgataatttaacccccgaaaggc                                                                                                                                                      >  1:562736‑M1/1‑164 (MQ=255)                                                                                         gggggtcagATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACGGAGGTTTTCCGCCAGACGCCACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTGACCATGCGGTCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCcacagtttcgggttttcgacgttcagacgtagtgtgacgcgatcggcataaccaccacgctcatcgataatttcaccgccgaaaggcgcg                                                                                                                                                   <  1:346464‑M1/247‑91 (MQ=255)                                                                                               cAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACTGCGGTTTTCCGCCAGACGCCACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTGACCATGCGGTCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCcacagtttcgggttttcgacgttcagacgtagtgtgacgcgatcggcataaccaccacgctcatcgataatttcaccgccgaaaggcgcggtgccgc                                                                                                                                            >  1:504131‑M1/1‑154 (MQ=255)                                                                                                   ggcGGGATGGCGTGGGCCGCGGCGGGGAGCGTCACCCTGAGGTTTTCCGCCAGACGCCACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTGACCATGCGGTCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCcacagtttcgggttttcgacgttcagacgtagtgtgacgcgatcggcataaccaccacgctcatcgataatttcaccgccgaaaggcgcggtgccgctggc                                                                                                                                        <  1:291792‑M1/250‑102 (MQ=255)                                                                                                    gCGGGATGGGGGGGGGCGCGGCGGGGGGCGTCACACTGAGGTTTTCCGCCAGACGCCACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTGCCCATGCGGTCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCcacattttcgggttttcgacgttcagacgaagtgttacgcgatcggcataaccaccacgctcatcgataatttcaccgccgaaaggcgcggtgccgctggc                                                                                                                                        <  1:507619‑M1/250‑102 (MQ=255)                                                                                                     cGGGATGGAGTGGGACGCGGCGGGGAGCGTCACACTGAGGTTTTCCGCCAGACGCCACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTGACCATGCGGTCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCcacagtttcgggttttcgacgttcagacgtagtgtgacgcgatcggcataaacaccacgctcttcgataatttccccgcccaaaggcgcggtgccgctggcg                                                                                                                                       >  1:100451‑M1/1‑148 (MQ=255)                                                                                                           ggCGTGGGACGCGGCGGGGGGCGTCACCCGGAGGTTTTCCGCCAGACGCCGCTGCTGCCGGGCGCTGATGTGCCCGGCTTCTGACCATGCGGTCGCGTTCGGTTGCACTACGCGTACTGGGAGCCAGAGTGGCCCGGCGCTCcacagtttcgggttttcgactttcagacgtagtgtgacgcgatcggcataacccccacgctcatcgataatttcaccgccgaaaggcgcggtgccgctggcgacctgc                                                                                                                                 <  2:453820‑M1/250‑109 (MQ=255)                                                                                                              gTGGGACGCGGCGGGGAGCGTCCCCCTGGGGTTTTCCGCCAGACGCCACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTGACCATGCGGTCGCGTTCGGTTGCCCTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCcacagtttcgggttttcgacgttcagacgtagtgtgacgcgatcggcataaccaccccgctcatcgataatttcaccgccgaaaggcgcggtgccgctggcgacctgcgttt                                                                                                                             <  1:1953‑M1/251‑113 (MQ=255)                                                                                                              gTGGGACGCGGCGGGGAGCGTCCCACTGAGGTTTCCCGCCAGACGCCACTGCTGCAAGGCGCTGATGTGCCCGGCTTCTGCCCATGCGGTCGCGTTCGGTTGCACTACGCGTACTGTGAGCAAGAGTTGCCCGGCGCTCcacattttcgggttttcgacgttcagacgtagttggacgcgatcggcataaccaccacgctcatcgataatttcaccgccgaaaggcgcggtgccgctggcgacctgcgttt                                                                                                                             <  1:261245‑M1/251‑113 (MQ=255)                                                                                                              gTGGGACGCGGCGGGGAGCGTCACACTGAGGTTTTCCGCCAGACGCCACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTGACCATGCGGTCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCcacagtttcgggttttcgacgttcagacgtagtgtgacgcgatc                                                                                                                                                                                                 <  2:195001‑M1/183‑45 (MQ=255)                                                                                                              gTGGGACGCGGCGGGGAGCGTCACACTGAGGTTTTCCGCCAGACGCCACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTGACCATGCGGTCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCcacagtttcggggtttcgacgttcagacgtagtgtgacgcgatc                                                                                                                                                                                                 >  1:195001‑M1/1‑139 (MQ=255)                                                                                                                gggACGCGGCGGGGAGCGTCCCACTGAGGTTTTCCGCCAGACGCCACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTGACCATGCGGTCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCcacagtttcgggttttcgacgttcagacgtagtgtgacgcgatcggcataaccaccacgctcatcgataatttcaccgccgaaaggcgcggtgccgctggcgacctgcgtttc                                                                                                                            >  1:413465‑M1/1‑137 (MQ=255)                                                                                                                          ggggAGCGTCACACTGAGGTTTTCCGCCAGACGCCACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTGACCATGCGGTCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCcacagtttcgggttttcgacgttcagacgtagtgtgacgcgatcggcataaccaccacgctcatcgataatttcaccgccgaaaggcgcggtgccgctggcgacctgcgtttcaccctgccat                                                                                                                  >  2:89888‑M1/1‑127 (MQ=255)                                                                                                                           gggAGCGTCACACTGAGGTTTTCCGCCAGACGCCACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTGACCATGCGGTCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCcacagtttcgggttttcgacgttcagacgtagtgtgacgcgatcggcataacccccacgctcatcgataatttcaccgccgaaaggcgcggtgccgctggcgacctgcgtttcaccctgccataa                                                                                                                <  1:63865‑M1/251‑126 (MQ=255)                                                                                                                           gggAGCGTCACACTGAGGTTTTCCGCCAGACGCCACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTGACCATGCGGTCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCcacagtttcgggttttcgacgttcagacgtagtgtgacgcgatcggcataaccaccacgctcatcgctaatttcaccgccgaaaggcgcggtgccgctggcgacctgcgtttcaccctgccata                                                                                                                 >  2:446056‑M1/1‑126 (MQ=255)                                                                                                                            ggAGCGTCACACTGAGGTTTTCCGCCAGACGCCACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTGACCATGCGGTCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCcacagtttcgggttttcgacgttcagccgtagtgtgacgcgatcggcataaccaccacgctcatcgataatttcaccgccgaacggcgcggtgccgctggcgacctgcgtttcaccctgccataa                                                                                                                >  2:504543‑M1/1‑125 (MQ=255)                                                                                                                                 gTCACACTGAGGTTTTCCGCCAGACGCCACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTGACAATGCGGTCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCcacagtttcgggttttcgacgttcagacgtagggtgacgcgatcggcataaccaccacgctcatcgataatttcaccgccgaaaggcgcggggccgctggcgacctgcgtttcaccctgccataaagaaac                                                                                                          >  2:207206‑M1/1‑120 (MQ=255)                                                                                                                                  tCACACTGAGGTTTTCCGCCAGACGCCACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTGACCATGCGGTCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCcacagtttcgggttttcgacgttcagacgtagtgtgacgcgatcggcataaccaccacgctcatcgataatttcaccgccgaaaggcgcggtgccgctggcgacctgcgtttcaccctgccataaagaaact                                                                                                         >  1:7778‑M1/1‑119 (MQ=255)                                                                                                                                      acTGAGGTTTTCCGCCAGACGCCACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTGACCATGCGGTCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCcacagtttcgggttttcgacgttcagacgtagtgtgacgcgatcggcataaccaccacgctcatcgataatttcaccgccgaaaggcgcggtgccgctggcgacctgcgtttcaccctgccataaagaaactgtt                                                                                                      >  2:8063‑M1/1‑115 (MQ=255)                                                                                                                                         gAGGTTTTCCGCCAGACGCCACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTGACCATGCGGTCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCcacagtttcgggttttcgacgttcagacgtagtgtgacgcgatcggcataaccaccacgctcatcgataatttcaccgccgaaaggcgcggtgccgctggcgacctgcgtttcaccctgccataaagaaactgttaccc                                                                                                  >  1:40580‑M1/1‑112 (MQ=255)                                                                                                                                                           ccACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTGACCATGCGGTCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCcacagtttcgggttttcgacgttcagacgtagtgtgacgcgatcggcataaccaccacgctcatcgataatttcaccgccgaaaggcgcggtgccgctggcgacctgcgtttcaccctgccataaagaaactgttacccgtaggtagtcacgcaact                                                                                >  1:425293‑M1/1‑94 (MQ=255)                                                                                                                                                            cACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTGACCATGCGGTCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCcacagtttcgggttttcgacgttcagacgtagtgtgacgcgatcggcataaccaccacgctcatcgataatttcaccgccgaaaggcgcggtgccgctggcgacctgcgtttcaccctgccataaagaaactgttacccgtaggtagtcacgcaactc                                                                               >  1:60717‑M1/1‑93 (MQ=255)                                                                                                                                                            cACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTGACCATGCGGTCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCcacagtttcgggttttcgacgttcagacgtagtgtgacgcgatcggcataaccaccacgctcatcgataatttcaccgccgaaaggcgcggtgccgctggcgacctgcgtttcaccctgccataaagaaactgttacccgtaggtagtcacgcaactc                                                                               >  1:10678‑M1/1‑93 (MQ=255)                                                                                                                                                            cACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTGACCATGCGGTCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGACCGGCGCTCcacagtttcgggttttcgacgttcagacgtagagtgacgcgatcggcataaccaccacgctcatcgataatttcaccgccgaaaggcgcggtgccgctggcgacctgcgtttcaccctgccataaagaaactgttacccgtaggtagtcacgcaactc                                                                               >  1:566384‑M1/1‑93 (MQ=255)                                                                                                                                                                      aGGCGCTGATGTGCCCGGCTTCTGACCATGCGGTCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCcacagtttcgggttttcgacgttcagacgtagtgtgacgcgatcggcataaccaccacgctcatcgataatttcaccgccgaaaggcgcggtgccgctggcgacctgcgtttcaccctgccataaagaaactgttacccgtaggtagtcacgcaactcgccgcacatc                                                                     >  1:348‑M1/1‑83 (MQ=255)                                                                                                                                                                         cgcTGATGTGCCCGGCTTCTGACCATGCGGTCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCcacagtttcgggttttcgacgttcagacgtagtgtgacgcgatcggcataaccaccacgctcatcgataatttcaccgccgaaaggcgcggtgccgctggcgacctgcgtttcaccctgccataaagaaactgttacccgtaggtagtcacgcaactcgccgcacatctg                                                                   >  1:190532‑M1/1‑80 (MQ=255)                                                                                                                                                                          gcTGATGTGCCCGGCTTCTGACCATGCGGTCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCcacagtttcgggttttcgacgttcagacgtagtgtgacgcgatcggcataaccaccacgctcatccataatttcaccgccgaaaggcgcggtgccgctggcgacctgcgtttcaccctgccataaagaaactgttacccgtaggtagtcacgcaactcgccgcacatctga                                                                  >  1:27226‑M1/1‑79 (MQ=255)                                                                                                                                                                                          tCTGGCCATGCGTGCGGGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCCCcccagtttcgggttttcgacgttcagaggtagtgtgacgcgatcggcataaccaccacgctcagcgataatttcaccgccgaaaggcgcggtgccgctgtcgacctgcgtttcaccctgccataaagaaactgttacccgtaggtagtcacgcaactcgccgcacatctgaacttcagcctccagt                                                   <  1:246418‑M1/249‑187 (MQ=255)                                                                                                                                                                                             aacCATGCGGTCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGGGCTCcacagtttcgggttttcgacgttcagacgtagtgtgacgcgatcggcataaccaccccgctcatcgataatttcaccgccgaaaggcgcggtgccgctggcgacctgcgtttcaccctgccataaagaaactgttacccgtaggtagtcacgcaactcgccgcacatctgaacttcagcctccagtacagc                                              <  1:399417‑M1/250‑192 (MQ=255)                                                                                                                                                                                              accagggggtcgcgttCGGTTGCATTAGGCGTACTGGGAGCCAGAGTGGCCCGGCGCTCcacagtttcgggttttcgacgttcagccgtggtgggacgcgatcggcataaccaccccgctcatcgataatttcaccgccgaaaggcgcggtgccgctggcgacctgcgtttcaccctgccataaagaaactgttacccgtaggtagtcacgcaactcgccgcacatctgaacttcagcctccagtacagcg                                             <  2:2432‑M1/244‑193 (MQ=255)                                                                                                                                                                                                    cGGTCGCGTTCGGGTGCACTACGCGTACTGTGAGCCCGAGCTGCCCGGCGCTAcacagtttcgggttttcgacgtccagacgtagtgtaacccgatcggcataaccaccccgctcatcgataatttcaccgccgaaaggcgcggcgccgctggcgacctgcgtctcaccctgccataaagcaactgttcccagtaggtagtcacgcaacacgccgcacatctgcacttcagcctccagtaaagcgcggatg                                       >  2:47912‑M1/1‑53 (MQ=255)                                                                                                                                                                                                           gTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCcacagtttcgggttttcgacgttcagacgtagtgtgacgcgatcggcataaccaccacgctcatcgataatttcaccgccgaaaggcgcggtgccgctggcgacctgcgtttcaccctgccataaagaaactgttacccg                                                                                                 >  1:310046‑M1/1‑46 (MQ=255)                                                                                                                                                                                                               ggTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCcacattttcggtttttcgacgttcagacgtagtgtgacgcgatcggcataaccaccacgctcatcgataatttcaccgccgaaaggcgcggtgccgctggcgacctgcgtttcaccctgccataaagaaactgttacccgtaggtagtcacgcaactcgccgcacatctgaacttcagcctccagtacagcgcggctgaaatcatcatt                            <  1:164269‑M1/251‑210 (MQ=255)                                                                                                                                                                                                                   gCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCcacagtttcgggttttcgacgttcagacgtagtgtgacgcgatcggcataaccaccacgctcatcgataatttcaccgccgaaaggcgcggtgccgctggcgacctgcgtttcaccctgccataaagaaactgttacccgtaggtagtcacgca                                                                                   >  2:154687‑M1/1‑38 (MQ=255)                                                                                                                                                                                                                   gCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCcacagtttcgggttttcgacgttcagacgtagtgtgacgcgatcggcataaccaccacgctcatcgataatttcaccgccgaaaggcgcggtgccgctggcgacctgcgtttcaccctgccataaagaaactgttacccgtaggtagtcacgca                                                                                   <  1:154687‑M1/192‑155 (MQ=255)                                                                                                                                                                                                                     aCTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCcacagtttcgggttttcgacgttcagacgtagtgtgacgcgatcggcataaccaccacgctcatcgataatttcaccgccgaaaggcgcggtgccgctggcgacctgcgtttcaccctgccataaagaaactgttacccgtaggtagtcacgcaactcgccgcacatctgaacttcagcctccagtacagcgcggctgaaatcatcattaaagcg                      >  1:338089‑M1/1‑36 (MQ=255)                                                                                                                                                                                                                         cgcgTACTGTGAGCCAGAGGTGCCCGGCGCTCcacagtttcgggttttcggcgttcagacgtagtgtgacgcgatcggcataaccaccacgctcatcgataatttcaccgccgacaggcgcggtaccgctggcgacctgcgtttcaccctgccataaagaaactgttacccgtaggtagtcacgcaactcgccgcacatctgaaattcagcctccagtacagcgcggctgaaatcatacttaaagcgagtg                  >  2:298983‑M1/1‑32 (MQ=255)                                                                                                                                                                                                                                    ggcCAGAGTTGCCCGGCGCTCcacagttgcgggttttcgacgttcagacgtagtgtgacgcgatcggcataacccccacgctcatcgataatttcaccgccgaaaggcgcggtgccgctggcgacctgcgtttcaccctgccataaagaaactgttacccgtaggtagtcacgcaactcgccgcacatctgaacttcagcctccagtacagcgcggctgaaatcatcattaaagcgagtggcaacatggaa       <  1:169494‑M1/250‑231 (MQ=255)                                                                                                                                                                                                                                    gcccagAGTTGCCCGCCGCTCcacatgtcggggttttcgacgtccagacgtggtgtgacccgatcggaataaccaccccgcccatcgaaaatttccccgccgaaaggcgcggtgccgctggcgacctgcgtttcaccctgccataaagaaactgttacccgtaggtagtcacgcaactcgccgcacatctgaacttcagcctccagtacagcgcggctgaaatcatcattaaagcgagtggcaacatggaa       <  1:24065‑M1/249‑231 (MQ=255)                                                                                                                                                                                                                                     gCCAGAGTTGCCGGGCCCTCcacagtgtcggttttccgacgttcagacggagtgtgacgcgatcggaataaccaccacgctcaccgataatttccccgccgaaaggcgcggtgccgctggcgacctgcgtttcaccctgccataaagaaactgttacccgtaggtagtcacgcaactcgccgcacatctgaacttcagcctccagtacagcgcggctgaaatcatcattaaagcgagtggcaacatggaaa      <  2:144425‑M1/251‑232 (MQ=255)                                                                                                                                                                                                                                        ggagtGGCCCGGCGCTCaacagtttggggttttggacgttcagccgtggtgggacgcgaccggcataaccaccacgctcatcgatattttcaccgccgaaaggcgcggtgccgctggcgacctgcgtttcaccctgccataaagaaactgttacccgtaggtagtcacgcaactcgccgcacatctgaacttcagcctccagtacagcgcggctgaaatcatcattaaagcgagtggcaacatggaaatcg   <  2:217740‑M1/250‑235 (MQ=255)                                                                                                                                                                                                                                         gagTTGCCCGGCGCTCcacagtttcgggttttcgccgttcagacgtagtgtgacgcgatcggcataaccaccacgctcatcgataatttcaccgccgaaaggcgcggtgccgctggcgacctgcgtttcaccctgccataaagaaactgttacccgtaggtagtcacgcaactcgccgcacatctgaacttcagcctcccgtacagcgcggctgacatcctcattaaagcgagtggcaacatggaaatcgc  >  2:59724‑M1/1‑16 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                            CGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACTGAGGTTTTCCGCCAGACGCCACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTGACCATGCGGTCGCGTTCGGTTGCACTACGCGTACTGTGAGCCAGAGTTGCCCGGCGCTCTCCGGCTGCGGTAGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGGTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGC  >  NC_000913/363989‑364471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑