Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,729,449	IS1 (+) +9 bp	noncoding (89‑97/160 nt) coding (1309‑1317/1323 nt)	xylZ ← xylA ←	small RNA XylZ xylose isomerase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1978503 =	NA (NA)	69 (1.550)	61/484	0.0	100%	noncoding (768/768 nt)	IS1	repeat region
	?	NC_000913	3729449 =	0 (0.000)					noncoding (97/160 nt) coding (1317/1323 nt)	xylZ xylA	small RNA XylZ xylose isomerase
*	?	NC_000913	3583428 =	NA (NA)	92 (2.070)	82/484	0.0	100%	noncoding (1/768 nt)	IS1	repeat region
	?	NC_000913	= 3729457	0 (0.000)					noncoding (89/160 nt) coding (1309/1323 nt)	xylZ xylA	small RNA XylZ xylose isomerase

GGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGTCATCCATATCACCACGTCAAAGGGTGAC  >  NC_000913/1978502‑1978752  |                                                                                                                                                                                                                                                          gggTAATGACTCCAACTTATTGCTAGTGTTTTATGTTCAGATAATGCCCGATGCCTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCCCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCCGCCCTCCGTCATCCATATCCCCACGTCAACGGGTGAc  >  2:201220/1‑251 (MQ=11)  |                                                                                                                                                                                                                                                          GGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGTCATCCATATCACCACGTCAAAGGGTGAC  >  NC_000913/1978502‑1978752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.