Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,252,952	IS2 (–) +5 bp	coding (42‑46/357 nt)	yhaI →	putative inner membrane protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3186096 =	NA (NA)	28 (0.690)	27/496	0.4	100%	noncoding (1/1331 nt)	IS2	repeat region
	?	NC_000913	3252952 =	0 (0.000)					coding (42/357 nt)	yhaI	putative inner membrane protein
*	?	NC_000913	= 3187426	NA (NA)	24 (0.590)	24/496	0.5	100%	noncoding (1331/1331 nt)	IS2	repeat region
	?	NC_000913	= 3252956	0 (0.000)					coding (46/357 nt)	yhaI	putative inner membrane protein

GGTGCGGAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTGCGTGACACCAGAGCAATTAACATTATTAGGATTTA  >  NC_000913/3187178‑3187676                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                           ggTGCGGAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGt                                                                                                                                                                                                                                                          >  1:120063/1‑251 (MQ=11) ggTGCGGAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGAGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAATTATAGGGGCAAATACAGt                                                                                                                                                                                                                                                          >  2:361840/1‑251 (MQ=11)     cGGAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTatc                                                                                                                                                                                                                                                      >  1:265584/1‑251 (MQ=31)         gTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTatcatc                                                                                                                                                                                                                                                   >  1:168305/1‑250 (MQ=33)           ccGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCAtt                                                                                                                                                                                                                                                <  1:110962/251‑1 (MQ=34)             ggAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGGATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTAAtcttcacagt                                                                                                                                                                                                                                              <  2:60165/251‑5 (MQ=2)                  gTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTtgtg                                                                                                                                                                                                                                         >  1:11519/1‑251 (MQ=38)                    aaaggAAGAGCAGAGAGGGTTGTGAAAACGATAAAGCGTGTCTAAATCAGTATCATGCCCAAACAAGTCGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTGCGAGCAGTATAACGAACGGCATCCGCAGAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCTGCAGCTGGCGTGAAATGGGTTAAGGGATGACAGATGTCTGGAAATATAGGTGCAAATCCAGTTattcagcgcgtaactat                                                                                                                                                                                                                                        <  2:97644/247‑16 (MQ=11)                    aaCGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGc                                                                                                                                                                                                                                        <  1:67/250‑1 (MQ=38)                           aaGCAGAGAGCTTCGTGAAAACTATAAAGCGTGGCTATATCAGTATCCTGCCCAAACCAGACGTGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGTATAGTGCGCTGGGTTATCGCTCGTCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTAGTTGTGCGCCAGCtt                                                                                                                                                                                                                                <  2:9969/250‑1 (MQ=11)                                gagagaatCGTGAAAACGATACAGCGTCACCACATCAGTCTCCTGCACAATCCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGCACGTGCATTATACCGAATGGCATTCGCTTAGCGCGCTGGGTTCTCGCTCGCCACGGGAATATCTGCGGCAGCGGGCATGTAATGGGTTAAGCGATAACAGTTTTCTGGAAATATAGGGGCCAATCCAGTTATCATCATTGGTTGAGCGTCAGCTTATGcc                                                                                                                                                                                                                           >  2:55482/8‑251 (MQ=14)                                    gCTTCGTTAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGTTTAACGGCAGCAAAGAACCTTGCAGAGGTGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGTTGGGTTATCGCGCGCCACGGGAATATCTGCGTCAGCGGTCTTGTAATGGGTTAAGTTATAACAGATTTCTGGAAAGATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTAttctgttg                                                                                                                                                                                                                       <  2:386602/251‑8 (MQ=14)                                         gTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTTGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGTGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTc                                                                                                                                                                                                                  <  2:396091/251‑1 (MQ=255)                                                cgataatacGTGACTACATCAGCCTCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGCGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTcaacaa                                                                                                                                                                                                             >  1:119693/9‑249 (MQ=255)                                                  aTAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGAt                                                                                                                                                                                                         <  1:191987/251‑1 (MQ=255)                                                         gcgACTACGTCAGTATCATACCCAAACCAAACGCGCTAACGGCAGCAAAAAACCTTGCAGAAGCGTTCGAGCAGTATAACGAATGGCATCCGCATAGCGCGCTGGATTATCGCCAGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGGGCTACAAGATGTCTGGAACTATAGGGGCAAATCCAGTTATCCTCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCg                                                                                                                                                                                                  >  2:221427/3‑251 (MQ=17)                                                           gACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCGTCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGTGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTTGAAATATAGGGGCAAATCCAGTGATCATCATTGGTTGTTCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGa                                                                                                                                                                                                 <  2:158367/250‑1 (MQ=255)                                                           gACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGTAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGGCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGGTGTGCg                                                                                                                                                                                                                                       <  2:173654/212‑1 (MQ=255)                                                           gACTACATCAGTATCATGCCCAAAACAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCg                                                                                                                                                                                                                                       >  1:173654/1‑212 (MQ=255)                                                           gACTACATCAGGATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGTAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTTTGCGTCAGCTTATGCCGGTGAATTCAACAAAGATTTAATCGaa                                                                                                                                                                                                <  2:62646/251‑1 (MQ=255)                                                            aCTACATCAGTAGCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGCTTGTGCGTCAGCTTATTCCGTTGAATTCAACAAAGATTTAATCGAAg                                                                                                                                                                                               <  1:175669/251‑1 (MQ=255)                                                                         cATGCCCAAACCAGACGGGTTAACGGCATCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCGCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTTATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGTTTGTGCGTCAGCTTATGCCGTGGAATTCAACAAAGATTTAATCGAAGCCGAAGATcttg                                                                                                                                                                                   <  2:75350/250‑4 (MQ=255)                                                                         cATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGa                                                                                                                                                                                  <  1:57105/251‑1 (MQ=255)                                                                         cATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCGGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATATCAGATGTCTGGAAATATAGGGGCAAATCCATTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAATATTTAATCGAAGCCGATGATCGTg                                                                                                                                                                                   <  2:166359/250‑1 (MQ=255)                                                                               cAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGt                                                                                                                                                                            <  1:304293/251‑1 (MQ=255)                                                                                       aCGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCttt                                                                                                                                                                    <  1:103528/251‑1 (MQ=255)                                                                                        cGGGTTAACGGCAGCAAAGGACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTAGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGAGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGCAACCGAAGATCGGGACAACGTTAACCTTTc                                                                                                                                                                   >  2:401358/1‑251 (MQ=18)                                                                                                   cagcaAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAAGTTGaaa                                                                                                                                                        <  1:80391/251‑1 (MQ=255)                                                                                                       aaaGGACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGAGGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGGCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAATCGTTAACCTTTCCCAATTTGAAACTGa                                                                                                                                                    <  2:238948/251‑1 (MQ=255)                                                                                                                 gCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCCGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCactt                                                                                                                                           >  2:220324/1‑247 (MQ=255)                                                                                                                         gTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCgg                                                                                                                                  >  1:124552/1‑251 (MQ=255)                                                                                                                                cATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAatat                                                                                                                           <  1:6981/251‑1 (MQ=255)                                                                                                                                    ataACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCa                                                                                                                        <  1:24791/250‑1 (MQ=255)                                                                                                                                       aCGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTGCCCGTCGGCAAATATTCACTaa                                                                                                                    <  1:353937/251‑1 (MQ=255)                                                                                                                                               cATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTg                                                                                                            >  2:172789/1‑251 (MQ=255)                                                                                                                                                         gtacgcTGGGTTATAGCTCGCCACGGGAATATATGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCACCTCCCGTTATCATCATTGGTTGTGCGTCAGATTATGCCGTTGAATTCAAAAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATtaataat                                                                                                   >  2:170719/4‑250 (MQ=38)                                                                                                                                                         gTGCGCTGGGTTAGCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTAGCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATtaataata                                                                                                  <  1:145376/251‑1 (MQ=255)                                                                                                                                                                   ttATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTAt                                                                                                                                                                                                                              <  1:28544/117‑1 (MQ=255)                                                                                                                                                                   ttATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTATGGCAATATAGGGGCAAATCCAGTTATAATCATTGGTTGTGCGTCAGCTTAt                                                                                                                                                                                                                              >  2:28544/1‑117 (MQ=17)                                                                                                                                                                    tATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGcc                                                                                        <  2:355161/250‑1 (MQ=255)                                                                                                                                                                             ccACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCATTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAAGTTGAAACTGATGGCCAATTACCCGTCGGCAAAGATTCACTAAGCGCTCTGATTAATAATAAGAGGACGCCAATCCACCtt                                                                              <  1:441242/251‑1 (MQ=255)                                                                                                                                                                             ccACGGGAATATCTGCGGCAGCGGGCTTGGAATGTGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAAGCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTGCCCAAGTTGAAACTGATGGCCAATTACCCATCGGCAAATATTCACTAAGCACTCTGAGTAAGAAGAAGGGGACTCCAAGCCACCtt                                                                              <  2:27848/251‑1 (MQ=38)                                                                                                                                                                                 gggAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGAcc                                                                          <  1:353558/251‑1 (MQ=255)                                                                                                                                                                                                     gCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCTAAGCCGAAGTTCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTTATTAATAATAAGAGGACGCCAATCCACCTTTACCTCCAATGGGTATTAATTGAt                                                      <  1:420354/251‑1 (MQ=255)                                                                                                                                                                                                      cTTGTAATGGGCTAAGTGCTAACAGATGTCTGGAAATCTAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCAGTTGTATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCACTGGGTATTAATTGAt                                                      >  2:291416/1‑250 (MQ=255)                                                                                                                                                                                                        tGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATAATCATTGGTTGTGCGTCAGCTTATGCCGATGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAAACTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGAAGCCAATCCACCTTCACCTCCAATGGGTATTAAtttctaac                                                   >  2:243717/1‑245 (MQ=255)                                                                                                                                                                                                         gTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAAc                                                   <  2:124552/250‑1 (MQ=255)                                                                                                                                                                                                              gggTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACt                                             >  1:317297/1‑251 (MQ=255)                                                                                                                                                                                                                gTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGc                                           <  1:361840/251‑1 (MQ=255)                                                                                                                                                                                                                  tAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGc                                           >  1:54290/1‑249 (MQ=255)                                                                                                                                                                                                                     gTGATAACAGATGTCTGGAAATATAGGTGCCAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCTTTGAATTCAACAAAGATTTAATCGAAGTCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAACTACCCTTCCGCAAATATTCACTAAGCACTCTGATTAATAATAAGATGACGCCAATCCATCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGttt                                      <  1:36083/251‑1 (MQ=255)                                                                                                                                                                                                                          aaCAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTGCGTg                                 <  2:120063/251‑1 (MQ=255)                                                                                                                                                                                                                           aCAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTGCGTGa                                <  2:265584/251‑1 (MQ=255)                                                                                                                                                                                                                               aTGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACGAAGCTCTCTGATTAATAATAATAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTGCGTGACAcc                            <  2:199617/251‑1 (MQ=255)                                                                                                                                                                                                                                tGTCTGGAGATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGCTAACCAAACTGCAGTTTGCGTGACAcc                            >  2:167560/1‑250 (MQ=255)                                                                                                                                                                                                                                 gTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATATCCAAACTGCAGTTTGCGTGACACCag                          <  2:168305/251‑1 (MQ=255)                                                                                                                                                                                                                                 gTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTGCGTGACACCa                           <  1:182821/250‑1 (MQ=255)                                                                                                                                                                                                                                   cTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGATTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCACATGGGTATTAATTGTTAACAAAACTGCAGTTTGAGTTACACCag                          >  2:259481/1‑249 (MQ=255)                                                                                                                                                                                                                                     ggAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTGCGTGACACCagag                        <  1:395004/249‑1 (MQ=255)                                                                                                                                                                                                                                     ggAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTGCGTGACACCAGAGc                       >  1:233392/1‑250 (MQ=255)                                                                                                                                                                                                                                                   aaaTCCAGTTATCATCATTGGTTGTGCGTCAGCTTCTGCCGTTGCATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAACCGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTGCGTGACACCAGAGCAATTAACattatt          >  2:446336/1‑249 (MQ=255)                                                                                                                                                                                                                                                       ccAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTGCGTGACACCAGAGCAAGTAACATTATTAGGAt     <  1:157384/250‑1 (MQ=255)                                                                                                                                                                                                                                                         aGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGACTTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTACCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTGCGTGACACCAGAGCAATTAACATTATTAGGAtttc  >  2:189883/1‑250 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                           GGTGCGGAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTGCGTGACACCAGAGCAATTAACATTATTAGGATTTA  >  NC_000913/3187178‑3187676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.