Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,698,402	IS30 (+) +2 bp	coding (148‑149/636 nt)	pgpC ←	phosphatidylglycerophosphatase C

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1470516	NA (NA)	31 (0.770)	31/492	0.3	96.9%	noncoding (1221/1221 nt)	IS30	repeat region
	?	NC_000913	2698402 =	1 (0.030)					coding (149/636 nt)	pgpC	phosphatidylglycerophosphatase C
*	?	NC_000913	= 2698403	1 (0.020)	31 (0.760)	28/496	0.4	96.9%	coding (148/636 nt)	pgpC	phosphatidylglycerophosphatase C
	?	NC_000913	= 4508679	NA (NA)					noncoding (1/1221 nt)	IS30	repeat region

ACTGTCAGCACCGGCGTTAAAGTTTACTTCTGCGATCCTCAGAGTCCTTGGCAGCGGGGAACAAATGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACACTGTTGAAATTCAGAATTTA‑GGTTTTGCTTTTGTTACTGGTGAAAATACAACAGGTATAAATAGTGGCACGATCTCGTTATTACAAAATGGTAAAGATCCGGCACCGTCTCCCATTGTTTTACTGGCTACTAACGGAGGGAGCGCCACTAATGCAGGTACGATCACAGGTAAAGTGACGGAACAACATAGCGTATTTAACAAGTATTCAACGGGCACATCGAATTCATTTATTTTTAATAACGATGTC  >  NC_000913/1470270‑1470764                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                          aCTGTCAGCACCGGCGTTAAAGTTTACTTCGGCGATCCTCAGAGGCCTTGGCAGCGGGGAACAAATGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTTAAAGGGGTGTTGCATTGACAGATTGAATCTACACTGt                                                                                                                                                                                                                                                       <  2:407348/251‑1 (MQ=14)        gCACCGGCGTTAAAGTTTACTTCTGCGATCCTCAGAGTCCTTGGCAGCGGGGAACAAATGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAATAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACACTGGTGAAAtt                                                                                                                                                                                                                                                <  1:155376/251‑1 (MQ=17)                gTTAAAGTTTACTTCTGCGATCCTCAGAGTCCTTGGCAGCGGGGAACAAATGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATGATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACACTGTTGAAATTCAGAAttt                                                                                                                                                                                                                                        >  1:412975/1‑251 (MQ=38)                   aaaGTTTACTTCTGCGATCCTCAGAGTCCTTGGCAGCGGTGAACAAATGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACACTGTTGAAATTCAGAATTTact                                                                                                                                                                                                                                     <  1:315547/251‑3 (MQ=255)                                 ggATCCTCAGAGTCCTTGGCAGCGGGGAACAAAAGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACTCCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAAGCTACACTGTTGAAAGTCAGAATTTA‑GGTTTTGCTTTTGTTa                                                                                                                                                                                                                      <  2:72578/250‑1 (MQ=255)                                  gATCCTCAGAGTCCTTGGCAGCGGGGAACAAATGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACACTGTTGAAATTCAGAATTTA‑GGTTTTGCTTTTGTTAc                                                                                                                                                                                                                     <  1:443804/251‑1 (MQ=255)                                   aTCCTCAGAGTCCTTGGCAGCGGGGAACAAATGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATTTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACACTGTTGAAATTCAGAATTTA‑GGTTTTGCTTTTGTTACt                                                                                                                                                                                                                    <  2:107026/251‑1 (MQ=255)                                                 tGGCAGCGGGGAACAAATGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTTAAGTTCAAAACACCGAAAGAGATAATTTAAAGGTGTGTTGCATTGACAGATTGAATCTACACTGTTGAAATTCATAATTTA‑TGTTTTGCTTTTGTTACTGGTGAAAATacaa                                                                                                                                                                                                       <  1:208209/250‑1 (MQ=255)                                                                aaTGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAA‑GACATGTCTTTACCAATATACTCAACATGAACTAGATCTTGTTTCTGCTCAGCTAATCAACATACCGAGAAAGACAGTGAAGTTCAAAACACCGAAAGAGATAATTGAGATGGGTGTTGCTTTTACAGATTTAATCTACACTGAGGAAATGTAGAAATTA‑GGTATTGCTTTTTTTTTTGGGGAAAATGTAAGAGGGATAAATAGTGGc                                                                                                                                                                                      <  2:444299/251‑1 (MQ=2)                                                                         acaAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACACTGTTGAAATTCAGAATTTA‑GGTTTTGCTTTTGTTACTGGTGAAAATACAACAGGTATAAATAGTGGCACGAtctc                                                                                                                                                                              <  1:39570/251‑1 (MQ=255)                                                                                     aTTCGGCAGTACTTTCCTAAAAAGACATGTCTAGCCCAATATACTCAACATGAACTAGATATGGTTGCAGCTCAGCTAAACAACAGACCGTGAAAAAAACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGCGTTGCATTGACAGATTGCATCTACACTGTTGAAATTCAGAATTTA‑GGTTTTGCTTTTGTTACTGGTGAAAATACAACAGGTATAAATAGTGGCACGATCTCGTTATTACaaa                                                                                                                                                                   >  2:373495/1‑250 (MQ=21)                                                                                            aGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAATAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTTTTGCATTGACAGATTGAATCTACACTGTTGAAATTCAGAATTTA‑GGTTTTGCTTTTGTTACTGGTGAAAATACAACAGGTATAAATAGTGGCACGATCTCGTTATTTCAAAATGGTaaa                                                                                                                                                           <  2:45450/251‑1 (MQ=255)                                                                                                       aaaaaGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACACTGTTGAAATTCAGAATTTA‑GGTTTTGCTTTTGTTACTGGTGAAAATACAACAGGTATAAATAGTGGCACGATCTCGTTATTACAAAATGGTAAAGATCCGGCAcc                                                                                                                                                <  1:208068/251‑1 (MQ=255)                                                                                                            gACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACACTGTTGAAATTCAGAATTTA‑GGTTTTGCTTTTGTTACTGGTGAAAATACAACAGGTATAAATAGTGGCACGATCTCGTTATTACAAAATGGTAAAGATCCGGCACCGtctc                                                                                                                                           <  1:16114/251‑1 (MQ=255)                                                                                                              cATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACACTGTTGAAATTCAGAATTTA‑GGTTTTGCTTTTGTTACTGGTGAAAATACAACAGGTATAAATAGTGGCACGATCGCGTTATTACAAAATGGTAAAGATCCGGCACCGTCTccc                                                                                                                                         <  1:45671/251‑1 (MQ=255)                                                                                                                             ataCTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACACTGTTg                                                                                                                                                                                                                                                     <  2:273830/129‑1 (MQ=35)                                                                                                                             ataCTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACACTGTTg                                                                                                                                                                                                                                                     >  1:273830/1‑129 (MQ=35)                                                                                                                               aCTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACACTGTTGAAATTCAGAATTTA‑GGTTTTGCTTTTGTTACTGGTGAAAATACAACAGGTATAAATAGTGGCACGATCTCGTTATTACAAAATGGTAAAGATCCGGCACCGTCTCCCATTGTTTTACTGGcta                                                                                                                         <  1:424284/250‑1 (MQ=255)                                                                                                                                          aCTAGATCTGGTTGCTGCCCAGCTAAACAACAGACCGAGAAAGGCACTGAAGTTCAAAACCCCGAAAGAGATAATTGAAAGGGGTGGTGCATTGACAGATTGAATCTTCACTGTTGAAATTCAGAATTTA‑GGTTTTGCTTTTGTTACTGGTGAAAATACAACATGTATAAATAGTGGCACGATCTCGTTATTACAAAATGGTAAAGATCCGGCACCGTCGCCCATTTTTTTACTGTCGACTAACgtaggga                                                                                                             <  2:25099/251‑1 (MQ=255)                                                                                                                                                       gctgctCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACACTGTTGAAATTCAGAATTTA‑GGTTTTGCTTTTGTTACTGGTGAAAATACAACAGGTATAAATAGTGGCACGATCTCGTTATTACAAAATGGTAAAGATCCGGCACCGTCTCCCATTGTTTTACTGGCTACTAACGGAGGGAGCGCCACTAATGc                                                                                                <  2:168919/251‑1 (MQ=255)                                                                                                                                                         tgctCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACACTGTTGAAATTc                                                                                                                                                                                                                                               >  2:245451/1‑107 (MQ=255)                                                                                                                                                         tgctCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACACTGTTGAAATTc                                                                                                                                                                                                                                               <  1:245451/107‑1 (MQ=255)                                                                                                                                                             cAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATGATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACACTGTTGAAATTCAGAATTTA‑GGGTTTGCTTTTGTTACTGGTGAAAATACAACAGGTATAAATAGTGGCACGATCGCGTTATTACAAAATGGTAAAGATCCGGCACCTTCTCCCATTGTTTTACTGGCTACGAACGGAGGGAGCGCCCCTAATGCAGGTAc                                                                                          <  2:412975/251‑1 (MQ=255)                                                                                                                                                                                    gactcTGAAGTGCAAAACAACGAAAGAGATAATTGAAAGGGGGGTTGCATTGACAGATTGAATCTACACTGTTGAAATTTATAATTTATGGTGTT‑CTTTTGTTACTGTTGAAAATACAACAGGTATAAATAGTGGCACGATCTCGTTATTACAAAATGGTAAAGTTCCTGCACCGTCTCCCATTGTTTTACTGGCTGCTAACGGAGGGTGCGTCAATAATGCAGTTACGGTCACAGGTAAAGTgtcgtaac                                                                   <  2:398018/247‑8 (MQ=255)                                                                                                                                                                                    gACACTGCAGTTCAACACACAGAAAGCGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACACTGTTGAAATTCAGAATTTA‑GGTTTTGCTTTTGTTACTGGTGAAAATACAACAGGTATAAATAGTGGCACGATCTCGTTATTACAAAATGGTAAAGATCCGGCACCGTCTCCCATTGTTTTACTGGCTACTAACGGAGGGAGCGCCACAAATGCAGGTACGATCACAGGTAAAGTGACGTaac                                                                   >  2:275913/1‑251 (MQ=255)                                                                                                                                                                                          gAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACACTGTTGAAATTCAGAATTTA‑GGTTTTGCTTTTGTTACTGGTGAAAATACAACAGGTATAAATAGTGGCACGATCTCGTTATTACAAAATGGTAAAGATCCGGCACCGTCTCCCATTGTTTTACTGGCTACTAACGGAGGGAGCGCCACTAATGCAGGTACGATCACAGGTAAAGTGACGGAacaaca                                                               <  2:118915/249‑1 (MQ=255)                                                                                                                                                                                                              agaTAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACACTGTTGAAATTCAGAATTTA‑GGTTTTGCTTTTGTTACTGGTGAAAATACAACAGGTATAAATAGTGGCACGATCTCGTTATTACAAAATGGTAAAGATCCGGCACCGTCTCCCATTGTTTTACTGGCTACTAACGGAGGGAGCGCCACTAATGCAGGTACGATCACAGGTAAAGTGACGGAACAACATAGCGTATTTAACAAGTATTc                                          >  1:350396/1‑250 (MQ=255)                                                                                                                                                                                                                   aTTGAAAGGGGTGTTGCATTGACAGATTGAATCTACACTGTTGAAATTCAGAATTTA‑GGTTTTGCTTTTGTTACTGGTGAAAATACAACAGGTATAAATAGTGGCACGATCTCGTTATTACAAAATGGTAAAGATCCGGCACCGTCTCCCATTGTTTTACTGGCTACTAACGGAGGGAGCGCCACTAATGCAGGTACGATCACAGGTAAAGTGACGGAACAACATAGCGTATTTAACAAGTATTCAACggg                                    >  1:71859/1‑251 (MQ=255)                                                                                                                                                                                                                                     ttGACAGATTGAATCTACACTGTTGAAATTCAGAATTTA‑GGTTTTGCTTTTGTTACTGGTGAAAATACAACAGGTATAAATAGTGGCACGATCTCGTTATTACAAAATGGTAAAGATCCGGCACCGTCTCCCATTGTTTTACTGGCTACTAACGGAGGGAGCGCCACTAATGCAGGTACGATCACAGGTAAAGTGACGGAACAACATAGCGTATTTAACAAGTATTCAACGGACACATCGAATTCatttat                  >  1:276920/1‑251 (MQ=255)                                                                                                                                                                                                                                           gATTGAATCTACACTGTAGAAATTCAGAATTTA‑GGTGTTGCTGTTGTTACTGGTGAAAAGACAACAGGTATAAATAGTGGCTCGATCTCGTTATTACAAAATGGTAAAGATCCGGCACCGTCGCTGTTAAGTTTACTGGCTACTAACGGGGGGAGCGTCACTAATTCAAGTACGATCACAGGTAAAGTGACGTAGCAACATAGCGGATTTAACGAGTATTCAACGGGCACATCGAATTCATTTATTTTtaa            <  1:197962/251‑1 (MQ=255)                                                                                                                                                                                                                                                      cacTGTTGAAATTCAGAATTTA‑GGTTTTGCTTTTGTTACTGGTGAAAATACAACAGGTATAAATAGTGGCACGATCTCGTTATTACAAAATGGTAAAGATCCGGCACCGTCTCCCATTGTTTTACTGGCTACTAACGGAGGGAGCGCCACTAATGCAGGTACGATCACAGGTAAAGTGACGGAACAACATAGCGTATTTAACAAGTATTCAACGGGCACATCGAATTCATTTATTTTTAATAACGATGTc  >  1:196355/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                          ACTGTCAGCACCGGCGTTAAAGTTTACTTCTGCGATCCTCAGAGTCCTTGGCAGCGGGGAACAAATGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACACTGTTGAAATTCAGAATTTA‑GGTTTTGCTTTTGTTACTGGTGAAAATACAACAGGTATAAATAGTGGCACGATCTCGTTATTACAAAATGGTAAAGATCCGGCACCGTCTCCCATTGTTTTACTGGCTACTAACGGAGGGAGCGCCACTAATGCAGGTACGATCACAGGTAAAGTGACGGAACAACATAGCGTATTTAACAAGTATTCAACGGGCACATCGAATTCATTTATTTTTAATAACGATGTC  >  NC_000913/1470270‑1470764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.