Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	4,285,976	IS150 (+) +3 bp	coding (1394‑1396/1959 nt)	acs ←	acetyl‑CoA synthetase (AMP‑forming)

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3720633 =	NA (NA)	35 (1.330)	33/486	0.1	94.6%	noncoding (1/1443 nt)	IS150	repeat region
	?	NC_000913	= 4285978	2 (0.080)					coding (1394/1959 nt)	acs	acetyl‑CoA synthetase (AMP‑forming)
*	?	NC_000913	= 3722075	NA (NA)	38 (1.440)	36/488	0.0	100%	noncoding (1443/1443 nt)	IS150	repeat region
	?	NC_000913	4285976 =	0 (0.000)					coding (1396/1959 nt)	acs	acetyl‑CoA synthetase (AMP‑forming)

CCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCG  >  NC_000913/4285726‑4286223                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                        ccTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCACAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGAt                                                                                                                                                                                                                                                         >  2:114947/1‑251 (MQ=255)    acgacGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTAGCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTa                                                                                                                                                                                                                                                       <  2:180502/247‑1 (MQ=255)       acGGCGGCTTCGGCAATCTTCGTATGCGCCACCAGCGCCTACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGGTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGAGTAtgta                                                                                                                                                                                                                                                   >  2:107825‑M2/1‑247 (MQ=255)            ggcTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGTCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgca                                                                                                                                                                                                                                              >  2:63899‑M2/1‑242 (MQ=255)               ggcaattgtctggataatgctgtggtggagtgtttctttggaaccttaaagtcggagtgtttttatcttgatgagttcagtaatataagcgaactgaaggatgctgttacggaatatattgaatactacaacagcagaagaattagcctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCtt                                                                                                                                                                                                                                           >  2:17729‑M2/237‑251 (MQ=255)                   attgtctggataatgctgtggtggagtgtttctttggaaccttaaagtcggagtgtttttatcttgatgagttcagtaatataagcgaactgaaggatgctgttacggaatatattgaatactacaacagcagaagaattagcctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGt                                                                                                                                                                                                                                       <  2:242849‑M2/19‑1 (MQ=255)                          tCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCTAAACAGCGTACGCGCCTGGCCCGGCCAGGAGTCGGTGATTAtgtactgctcccagtttgtt                                                                                                                                                                                                                                   >  2:32537‑M2/1‑228 (MQ=255)                           cGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttgga                                                                                                                                                                                                                                >  2:204267‑M2/1‑227 (MQ=255)                                   ccaccaGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGCGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGCAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaat                                                                                                                                                                                                                       <  1:137030‑M2/251‑33 (MQ=255)                                     tggtggagtgtttatttggacccttaaagtcggagtgtttttatcttgatgcgttcagtaatataagcgaactgaaggatgctgttacggaatatattgaatactacaacagcagaagaattagcctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGtt                                                                                                                                                                                                                     >  2:131157‑M2/215‑251 (MQ=255)                                       gtggagtgtttctttggaaccttaaagtcggagtgtttttatcttgatgagttcagtaatataagcgaactgaaggatgctgttacggaatatattgaatactacaacagcagaagaattagcctgaaattaaaaggtctgactccaattgcatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTAc                                                                                                                                                                                                                   >  2:198958‑M2/213‑251 (MQ=255)                                            gtgtttctttggaaccttaaagtcggagtgtttttatcttgatgagttcagtaatataagcgaactgaaggatgctgttacggaatatattgaatactacaacagcagaagaattagcctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCg                                                                                                                                                                                                              >  2:115120‑M2/208‑251 (MQ=255)                                                    ttggaaccttaaagtcggagtgtttttatcttgatgagttcagtaatataagcgaactgaaggatgctgttacggaatatattgaatactacaacagcagaagaattagcctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCg                                                                                                                                                                                                        <  2:224481‑M2/50‑1 (MQ=255)                                                         accttaacgtcggcgtgtttttatcttgatgcgttcagtactataatcgacctgcaggatgctgttacggaatatattgcatactacaacagcagaagcattagcctgaaattaacaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccacctttttgggttcagtacaTTACCAGTCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCTaccatcc                                                                                                                                                                                                 >  2:41763‑M2/195‑248 (MQ=255)                                                          gCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtc                                                                                                                                                                                                   >  1:271206‑M2/1‑196 (MQ=255)                                                             gTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCGGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatgtaatagccccgaatatgtcaaagcc                                                                                                                                                                                             >  1:82554‑M2/1‑193 (MQ=255)                                                                    gACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatac                                                                                                                                                                                      >  1:195613‑M2/1‑186 (MQ=255)                                                                     aCGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatacc                                                                                                                                                                                     <  2:62526‑M2/251‑67 (MQ=255)                                                                        gTGACCGGAGACGCTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTCCGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccacaataccctt                                                                                                                                                                                  <  2:253281‑M2/251‑70 (MQ=255)                                                                         gtttttatcttgatgagttcagtaatataagcgaactgaaggatgctgttacggaatatattgaatactacaacagcagaagaattagcctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCgaa                                                                                                                                                                                 <  1:7772‑M2/73‑1 (MQ=255)                                                                           ttttatcttgatgagttcagtaatataagcgaactgaaggatgctgttacggaatatattgaatactacaacagcagaagaattagcctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCga                                                                                                                                                                                  >  1:3636‑M2/177‑248 (MQ=255)                                                                              tatcttgatgagttcagtaatataagcgaactgaaggatgctgttacggaatatattgaatactacaacagcagaagaattagcctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttgggggcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAAc                                                                                                                                                                             <  2:154478‑M2/77‑1 (MQ=255)                                                                                 cttgatgagttcagtaatataagcgaactgaagaatgctgttacggaatatattgaatactacaacagcagaagaattagcctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGATACCTTCGTTATCGACCAGCGCTGGTTGCACGCCGAAGAACGGAc                                                                                                                                                                         >  1:76893‑M2/171‑251 (MQ=255)                                                                                   tgatgagttcagtaatataagctaactgaaggatgctgttacggaatatattgaatactacaacagcagaagaattagcctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGAc                                                                                                                                                                         <  2:149616‑M2/81‑1 (MQ=255)                                                                                         gttcagtaatataagcgaactgaaggatgctgttacggaatatattgaatactacaacagcagaagaattagcctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGcc                                                                                                                                                                 >  1:54959‑M2/163‑251 (MQ=255)                                                                                             gtcgtcCACACGCCCGGCTATACAGTAATAGCCATCTTCATCGCGCCGCGCGCCCTAGCCGCTGAAATACATCTTTTTGAAGGTCGAGAAGTACGACTGTTAAAACCGTTCGTGATCGCCCAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcaccaattttgttggacgatgaaatggaatagcccctaatatgtaaaagccaaaatacccttttgaaaagagccttgaagt                                                                                                                                                               <  2:124316‑M2/249‑89 (MQ=255)                                                                                                  ccACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatacccttttgaaaagcgccttgaagtcgtgaa                                                                                                                                                         >  2:117523‑M2/1‑156 (MQ=255)                                                                                                    ccacgCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCTGCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatacccttttgaaaagcgccttgaattcgtgaatca                                                                                                                                                      >  2:104842‑M2/2‑154 (MQ=255)                                                                                                      ccgCCCGGTTATCCAGTACTAGCCATCTTCTTCGCGACGCGCGCCGTCGCCTCTGAAATACATATTTTTGAAGTTGGAGAAGTTGTTCTGTGCAAAACGCTCGTGATCTCCAAACAGCGTACGCGCCTCACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatgtaatagcccctaatatttcaaatccaaaatacccttttgaaacgcgccttgaagacgtgaatcact                                                                                                                                                    >  1:177473‑M2/2‑152 (MQ=255)                                                                                                         cccGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatacccttttgaaaagcgccttgaagtcgtgaatcactact                                                                                                                                                 >  1:62935‑M2/1‑149 (MQ=255)                                                                                                            ctgaaggatgctgttacggaatatattgaatactacaacagcagaagaattagcctgaaattaaaaggtatgactccaattgaatatcgaaatcagacctatatgcctcgtgtttaactgtcaaactttttgggatcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCgg                                                                                                                                              >  1:128852‑M2/144‑251 (MQ=255)                                                                                                                aggatgctgttacggaatatattgaatactacaacagcagaagaattagcctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAgc                                                                                                                                          <  2:45869‑M2/112‑1 (MQ=255)                                                                                                                aTCCAGTAATAGCCAACGTTATCGCGACGCTCGCCGGCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatacccttttgaaaagcgccttgaagtcgtgaatcactacttcacaa                                                                                                                                           >  2:66733‑M2/1‑142 (MQ=255)                                                                                                                     gTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatacccttttgaaaagcgccttgaagtcgtgaatcactacttcacaactgatg                                                                                                                                     >  1:67629‑M2/1‑137 (MQ=255)                                                                                                                                atatcttgaatactacaacagcagaagaattagcctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTg                                                                                                                          >  2:156021‑M2/124‑251 (MQ=255)                                                                                                                                   tattgaatactacaacagcagaagaattagcctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTa                                                                                                                                                                                                                                               >  2:2977‑M2/121‑131 (MQ=255)                                                                                                                                   tattgaatactacaacagcagaagaattagcctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTa                                                                                                                                                                                                                                               <  1:2977‑M2/11‑1 (MQ=255)                                                                                                                                         atactacaacagcagaagaattagcctgaaattaaaaggtctgactccaattgactatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGACCCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGAGATCATGaa                                                                                                                  >  2:128559‑M2/115‑250 (MQ=255)                                                                                                                                                   gTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatacccttttgaaaagcgccttgaagtcgtgaatcactacttcacaactgatgatggttacaggatcatctcggcacgttttg                                                                                                       <  1:87833‑M2/251‑145 (MQ=255)                                                                                                                                                         agaattagcctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCt                                                                                                 >  2:157320‑M2/99‑251 (MQ=255)                                                                                                                                                            attagcctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGGGATCATGAAACCGCCGGTTTCGGTCTGcc                                                                                              >  1:248260‑M2/96‑251 (MQ=255)                                                                                                                                                                tACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatacccttttgaaaagcgccttgaagtcgtgaatcactacttcacaactgatgatggttacaggatcatctcggcacgttttggtgtcccccgaac                                                                                          <  2:184332‑M2/251‑158 (MQ=255)                                                                                                                                                                  ctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTAAGCTCGGTAGCGCCAGGCAGCGGGGTGAACATGAAACCGCCGGTTTCGGTCTGCCACCAgg                                                                                        >  2:246558‑M2/90‑251 (MQ=255)                                                                                                                                                                  cATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatacccttttgaaaagcgccttgaagtcgtgaatcactacttcacaactgatgatggttacaggatcatctcggcacgttttggtgtcccccgaaccc                                                                                        <  1:107339‑M2/251‑160 (MQ=255)                                                                                                                                                                           aaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaaatttttggggccagtacaTTACCAGGCTACCTTCCGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCCccaggtagcgccc                                                                                >  2:169246‑M2/81‑244 (MQ=255)                                                                                                                                                                            aaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGCCCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAAccgccg                                                                                                           <  1:47507‑M2/143‑1 (MQ=255)                                                                                                                                                                            aaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAAccgccg                                                                                                           >  2:47507‑M2/80‑222 (MQ=255)                                                                                                                                                                              ggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGCACCGGCTTTCAGCTCGGTACCGCCAGGCAGCGGGGTGATCATGAACCCGCCGGTTTCGGTCTGCCACCAGGTATCGaccacc                                                                             >  2:110030‑M2/78‑250 (MQ=255)                                                                                                                                                                                 ggAGAAGTAGGTCTGATCAAAACGTTCGTGATCTCCAAACCGCGTACGCGCCTGAACCGGACAGGAGTCGGTGATTAtgtactgaacccagtttgttggacgatgaaatggaatagcccctaatatggcaaagccaaaatacccttttgaaaagcgccttgaagtcgtgagtcacaacttcacaactgatgatggttacaggatcatctcggcacgttttggtgtccccagaacccaggtccggccatggg                                                                         <  2:61673‑M2/251‑175 (MQ=255)                                                                                                                                                                                   agaAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatacccttttgaaaagcgccttgaagtcgtgaatcactacttcacaactgatgatggttacaggatcatctcggcacgttttggtgtcccccgaacccaggtcaggacatgggtt                                                                       <  1:18445‑M2/251‑177 (MQ=255)                                                                                                                                                                                         aGGTCTGTTCAAAACTTTCGTGATCGCCATTCAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatacccttttgaaaagcgccttgaagtcgtgaatcactacttcacaactgatgatggttacaggatcatctcggcacgttttggtgtcccccgaacccaggtcaggacatgggttgccct                                                                  >  1:3758‑M2/1‑69 (MQ=255)                                                                                                                                                                                           gTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatacccttttgaaaagcgccttgaagtcgtgaatcactacttcacaactgatgatggttacaggatcatctcggcacgttttggtgtcccccgaacccaggtcaggacatgggttgccctcta                                                               >  1:62526‑M2/1‑67 (MQ=255)                                                                                                                                                                                               gTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatacccttttgaaaagcgccttgaagtcgtgaatcactacttcacaactgatgatggttacaggatcatctcggcacgttttggtgtcccccgaacccaggtcaggacattggttgccctctatga                                                            >  2:154899‑M2/1‑63 (MQ=255)                                                                                                                                                                                                         cagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGCACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTcc                                                 >  2:150570‑M2/51‑251 (MQ=255)                                                                                                                                                                                                         cagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGCCATTTCTCGTTGCCGATTTTTTTcc                                                 >  2:117979‑M2/51‑251 (MQ=255)                                                                                                                                                                                                             tGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatacccttttgaaaagcgccttgaagtcgtgaatcactacttcacaactgatgatggttacaggatcatctcggcacgttttggtgtcccccgaacccaggtcaggacatgggttgccctctatgaaaaacctggagaaa                                              <  2:172852‑M2/250‑202 (MQ=255)                                                                                                                                                                                                              gATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatacccttttgaaaagcgccttgaagtcgtgaatcactacttcacaactgatgatggttacaggatcatctcggcacgttttggtgtcccccgaacccaggtcaggacatgggttgccctctatgaaaaacatggagaaaa                                             >  2:43408‑M2/1‑48 (MQ=255)                                                                                                                                                                                                               tatatgcctcgtttttaactgtccatctttttggggtaagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTTTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCg                                                                                   <  1:147069‑M2/167‑1 (MQ=255)                                                                                                                                                                                                               tatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGGGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCg                                                                                   >  2:147069‑M2/45‑211 (MQ=255)                                                                                                                                                                                                                atatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTAcc                                           >  2:136531‑M2/44‑250 (MQ=255)                                                                                                                                                                                                                atatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTAcc                                           >  1:231442‑M2/44‑250 (MQ=255)                                                                                                                                                                                                                     aaaCAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatacccttttgaaaagcgccttgaagtcgtgaatcactacttcacaactgatgatggttacaggatcatctcggcacgttttggtgtcccccgcacccaggtcaggacatgggttgccctctatgaaaaacatggagaaaaaggttta                                      <  1:167516‑M2/250‑210 (MQ=255)                                                                                                                                                                                                                     aaaCAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatacccttttgaaaagcgccttgaagtcgtgaatcactacttcacaactgatgatggttacaggatcatctcggcacgttttggtgtcccccgaacccaggtcaggacatgggttgccctcta                                                               >  1:111066‑M2/1‑41 (MQ=255)                                                                                                                                                                                                                     aaaCAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatacccttttgaaaagcgccttgaagtcgtgaatcactacttcacaactgatgatggttacaggatcatctcggcacgttttggtgtcccccgaacccaggtcaggacatgggttgccctcta                                                               <  2:111066‑M2/225‑185 (MQ=255)                                                                                                                                                                                                                         aGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatacccttttgaaaagcgccttgaagtcgtgaatcactacttcacaactgatgatggttacaggatcatctcggcacgttttggtgtcccccgaacccaggtcaggacatgggttgccctctatgaaaaacatggagaaaaaggtttaattcc                                 <  1:208316‑M2/251‑215 (MQ=255)                                                                                                                                                                                                                         aGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatacccttttgaaaagcgccttgaagtcgtgaatcactacttcacaactgatgatggttacaggatcatctcggcacgttttggtgtcccccgaacccaggtcaggacatggggtgccctctatgaaaaacatggagaaaaaggtgtaattcc                                 >  2:137030‑M2/1‑37 (MQ=255)                                                                                                                                                                                                                           gtttaactgtcccactttttggggccagtacaTTACCAGGCTACCTTCGGAGGCCCCCTCCAGCGGGCTACATTCGTTTTCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGACGCACCGGCTTTCAGCTCGGTAGCGCACGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGAATCGACCACCGGACATTTCTCGTTGCCGCTTTTTTTCCCGTACCAATCCCACGCtt                               >  2:29598‑M2/33‑251 (MQ=255)                                                                                                                                                                                                                            gTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatacccttttgcaaagcgccttgaagtcgtgaatcactacttcacaactgatgatggttacaggatcatctcggcacgttttggtgtcccccgaacccaggtcagtacatgggttgccctctatgaaaaacatggagaaaaaggattaattccca                               <  2:3226‑M2/250‑217 (MQ=255)                                                                                                                                                                                                                              taactgtacaactttttggggtcaatacaTTACCAGGCTACCTTCGGTGGCCCACTCCAACGGGTTACCTTCGTTCTAGACCAGCGCCGTTTGCACGCCGAAGAACGAACGTGTTGCCGACCCTGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAACCCGCCGGTTTCGGTCTGCCACCAGACATCGACCACCGGACATTTCTCGTTCACGATTTTTTTCCCGTACCACTCCCACGCTtccc                            >  2:278852‑M2/30‑250 (MQ=255)                                                                                                                                                                                                                               gtccccatacccctccatcagtcggttattatggactgcccccatttttatggacgatgaaatggaatagccccttatatgtcaaagccaaaatacccttttgaaaagcgccttgaagtcgtgaatcactactgcacaactgatgatggtttcaggatcatctcggcacggtttggtgtcccccgaaaccaggtcaggacatgggttgccctctatgaaaaacatggagaaaaaggtttaattcccaaac                            >  2:102819‑M2/20‑31 (MQ=255)                                                                                                                                                                                                                                    tccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTa                       >  2:169778‑M2/24‑250 (MQ=255)                                                                                                                                                                                                                                       cccgttttggggtcattacaTCACCAGGCTCCCTTCGGTGGCCCCCGCCAGCGGGTTACCTGCGTTATGGCCCAGCGCGGGTTGCACGCCGAAGAACGGACGTGTGGCCGACCCGGCTTGCAGCGCGTTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTg                   <  2:125616‑M2/231‑1 (MQ=255)                                                                                                                                                                                                                                            tttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCg              >  1:68844‑M2/16‑251 (MQ=255)                                                                                                                                                                                                                                              cAGGAGTCGGGGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatacccttttgaaaagcgccttgaagtcgtgaatcactacttcacaactgatgatggttacaggatcatctcggcacgttttggtgtcccccgaacccaggtcaggacatgggttgccctctatgaaaaacatggagaaaaaggtttaattcccaaacctaaaggcgttagtgc            >  2:217581‑M2/1‑16 (MQ=255)                                                                                                                                                                                                                                               ggggtcagtacaTTACCAGGCTACCTTCGGTGGCCACCTCCAGCGGGTCCCCTTCCATATCGACCAGCGCCGGTTGCACGGCGACGACCGGCAGTGCTGCCGACCCGGCTTTAAGCTCGGTAGCGACAGGCAGCGCGGTGATCATGAAAACGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGCCATTTCTCGTTGCCGATTTTTTTCCCGTACCACTCCCACGCTTCCGGGATAATTGGCTCGccc           >  1:274297/12‑251 (MQ=255)                                                                                                                                                                                                                                                     agtacaGTACCAGGCTACCTTCGGTGGCCCCCCCCAGGGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTAAGCTGGGTAGCGCCGGGCGGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGaa     <  2:54959‑M2/245‑1 (MQ=255)                                                                                                                                                                                                                                                          aTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTCCCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCg  >  2:143896/1‑249 (MQ=255)                                                                                                                                                                                                                                                           ttACCAGCCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCCCCGTTATCAACCAGCGCCGGTTGCACGACGAACAAAGGACGTGTTGCCGAACCGGCTTTCAGATCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACTTTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCCCGCCTCCGGATTAATTGGCTCGCCCACGGAACCg  >  2:233482/1‑248 (MQ=255)                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                        CCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCG  >  NC_000913/4285726‑4286223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.