Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	4,285,976	IS150 (+) +3 bp	coding (1394‑1396/1959 nt)	acs ←	acetyl‑CoA synthetase (AMP‑forming)

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3720633 =	NA (NA)	35 (1.330)	33/486	0.1	94.6%	noncoding (1/1443 nt)	IS150	repeat region
	?	NC_000913	= 4285978	2 (0.080)					coding (1394/1959 nt)	acs	acetyl‑CoA synthetase (AMP‑forming)
*	?	NC_000913	= 3722075	NA (NA)	38 (1.440)	36/488	0.0	100%	noncoding (1443/1443 nt)	IS150	repeat region
	?	NC_000913	4285976 =	0 (0.000)					coding (1396/1959 nt)	acs	acetyl‑CoA synthetase (AMP‑forming)

ACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGA  >  NC_000913/4285729‑4286226                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                         acgacGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTAGCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTa                                                                                                                                                                                                                                                          <  2:180502/247‑1 (MQ=255)    acGGCGGCTTCGGCAATCTTCGTATGCGCCACCAGCGCCTACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGGTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGAGTAtgta                                                                                                                                                                                                                                                      >  2:107825‑M2/1‑247 (MQ=255)         ggcTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGTCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgca                                                                                                                                                                                                                                                 >  2:63899‑M2/1‑242 (MQ=255)            ggcaattgtctggataatgctgtggtggagtgtttctttggaaccttaaagtcggagtgtttttatcttgatgagttcagtaatataagcgaactgaaggatgctgttacggaatatattgaatactacaacagcagaagaattagcctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCtt                                                                                                                                                                                                                                              >  2:17729‑M2/237‑251 (MQ=255)                attgtctggataatgctgtggtggagtgtttctttggaaccttaaagtcggagtgtttttatcttgatgagttcagtaatataagcgaactgaaggatgctgttacggaatatattgaatactacaacagcagaagaattagcctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGt                                                                                                                                                                                                                                          <  2:242849‑M2/19‑1 (MQ=255)                       tCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCTAAACAGCGTACGCGCCTGGCCCGGCCAGGAGTCGGTGATTAtgtactgctcccagtttgtt                                                                                                                                                                                                                                      >  2:32537‑M2/1‑228 (MQ=255)                        cGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttgga                                                                                                                                                                                                                                   >  2:204267‑M2/1‑227 (MQ=255)                                ccaccaGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGCGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGCAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaat                                                                                                                                                                                                                          <  1:137030‑M2/251‑33 (MQ=255)                                  tggtggagtgtttatttggacccttaaagtcggagtgtttttatcttgatgcgttcagtaatataagcgaactgaaggatgctgttacggaatatattgaatactacaacagcagaagaattagcctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGtt                                                                                                                                                                                                                        >  2:131157‑M2/215‑251 (MQ=255)                                    gtggagtgtttctttggaaccttaaagtcggagtgtttttatcttgatgagttcagtaatataagcgaactgaaggatgctgttacggaatatattgaatactacaacagcagaagaattagcctgaaattaaaaggtctgactccaattgcatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTAc                                                                                                                                                                                                                      >  2:198958‑M2/213‑251 (MQ=255)                                         gtgtttctttggaaccttaaagtcggagtgtttttatcttgatgagttcagtaatataagcgaactgaaggatgctgttacggaatatattgaatactacaacagcagaagaattagcctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCg                                                                                                                                                                                                                 >  2:115120‑M2/208‑251 (MQ=255)                                                 ttggaaccttaaagtcggagtgtttttatcttgatgagttcagtaatataagcgaactgaaggatgctgttacggaatatattgaatactacaacagcagaagaattagcctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCg                                                                                                                                                                                                           <  2:224481‑M2/50‑1 (MQ=255)                                                      accttaacgtcggcgtgtttttatcttgatgcgttcagtactataatcgacctgcaggatgctgttacggaatatattgcatactacaacagcagaagcattagcctgaaattaacaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccacctttttgggttcagtacaTTACCAGTCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCTaccatcc                                                                                                                                                                                                    >  2:41763‑M2/195‑248 (MQ=255)                                                       gCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtc                                                                                                                                                                                                      >  1:271206‑M2/1‑196 (MQ=255)                                                          gTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCGGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatgtaatagccccgaatatgtcaaagcc                                                                                                                                                                                                >  1:82554‑M2/1‑193 (MQ=255)                                                                 gACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatac                                                                                                                                                                                         >  1:195613‑M2/1‑186 (MQ=255)                                                                  aCGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatacc                                                                                                                                                                                        <  2:62526‑M2/251‑67 (MQ=255)                                                                     gTGACCGGAGACGCTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTCCGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccacaataccctt                                                                                                                                                                                     <  2:253281‑M2/251‑70 (MQ=255)                                                                      gtttttatcttgatgagttcagtaatataagcgaactgaaggatgctgttacggaatatattgaatactacaacagcagaagaattagcctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCgaa                                                                                                                                                                                    <  1:7772‑M2/73‑1 (MQ=255)                                                                        ttttatcttgatgagttcagtaatataagcgaactgaaggatgctgttacggaatatattgaatactacaacagcagaagaattagcctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCga                                                                                                                                                                                     >  1:3636‑M2/177‑248 (MQ=255)                                                                           tatcttgatgagttcagtaatataagcgaactgaaggatgctgttacggaatatattgaatactacaacagcagaagaattagcctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttgggggcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAAc                                                                                                                                                                                <  2:154478‑M2/77‑1 (MQ=255)                                                                              cttgatgagttcagtaatataagcgaactgaagaatgctgttacggaatatattgaatactacaacagcagaagaattagcctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGATACCTTCGTTATCGACCAGCGCTGGTTGCACGCCGAAGAACGGAc                                                                                                                                                                            >  1:76893‑M2/171‑251 (MQ=255)                                                                                tgatgagttcagtaatataagctaactgaaggatgctgttacggaatatattgaatactacaacagcagaagaattagcctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGAc                                                                                                                                                                            <  2:149616‑M2/81‑1 (MQ=255)                                                                                      gttcagtaatataagcgaactgaaggatgctgttacggaatatattgaatactacaacagcagaagaattagcctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGcc                                                                                                                                                                    >  1:54959‑M2/163‑251 (MQ=255)                                                                                          gtcgtcCACACGCCCGGCTATACAGTAATAGCCATCTTCATCGCGCCGCGCGCCCTAGCCGCTGAAATACATCTTTTTGAAGGTCGAGAAGTACGACTGTTAAAACCGTTCGTGATCGCCCAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcaccaattttgttggacgatgaaatggaatagcccctaatatgtaaaagccaaaatacccttttgaaaagagccttgaagt                                                                                                                                                                  <  2:124316‑M2/249‑89 (MQ=255)                                                                                               ccACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatacccttttgaaaagcgccttgaagtcgtgaa                                                                                                                                                            >  2:117523‑M2/1‑156 (MQ=255)                                                                                                 ccacgCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCTGCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatacccttttgaaaagcgccttgaattcgtgaatca                                                                                                                                                         >  2:104842‑M2/2‑154 (MQ=255)                                                                                                   ccgCCCGGTTATCCAGTACTAGCCATCTTCTTCGCGACGCGCGCCGTCGCCTCTGAAATACATATTTTTGAAGTTGGAGAAGTTGTTCTGTGCAAAACGCTCGTGATCTCCAAACAGCGTACGCGCCTCACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatgtaatagcccctaatatttcaaatccaaaatacccttttgaaacgcgccttgaagacgtgaatcact                                                                                                                                                       >  1:177473‑M2/2‑152 (MQ=255)                                                                                                      cccGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatacccttttgaaaagcgccttgaagtcgtgaatcactact                                                                                                                                                    >  1:62935‑M2/1‑149 (MQ=255)                                                                                                         ctgaaggatgctgttacggaatatattgaatactacaacagcagaagaattagcctgaaattaaaaggtatgactccaattgaatatcgaaatcagacctatatgcctcgtgtttaactgtcaaactttttgggatcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCgg                                                                                                                                                 >  1:128852‑M2/144‑251 (MQ=255)                                                                                                             aggatgctgttacggaatatattgaatactacaacagcagaagaattagcctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAgc                                                                                                                                             <  2:45869‑M2/112‑1 (MQ=255)                                                                                                             aTCCAGTAATAGCCAACGTTATCGCGACGCTCGCCGGCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatacccttttgaaaagcgccttgaagtcgtgaatcactacttcacaa                                                                                                                                              >  2:66733‑M2/1‑142 (MQ=255)                                                                                                                  gTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatacccttttgaaaagcgccttgaagtcgtgaatcactacttcacaactgatg                                                                                                                                        >  1:67629‑M2/1‑137 (MQ=255)                                                                                                                             atatcttgaatactacaacagcagaagaattagcctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTg                                                                                                                             >  2:156021‑M2/124‑251 (MQ=255)                                                                                                                                tattgaatactacaacagcagaagaattagcctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTa                                                                                                                                                                                                                                                  >  2:2977‑M2/121‑131 (MQ=255)                                                                                                                                tattgaatactacaacagcagaagaattagcctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTa                                                                                                                                                                                                                                                  <  1:2977‑M2/11‑1 (MQ=255)                                                                                                                                      atactacaacagcagaagaattagcctgaaattaaaaggtctgactccaattgactatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGACCCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGAGATCATGaa                                                                                                                     >  2:128559‑M2/115‑250 (MQ=255)                                                                                                                                                gTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatacccttttgaaaagcgccttgaagtcgtgaatcactacttcacaactgatgatggttacaggatcatctcggcacgttttg                                                                                                          <  1:87833‑M2/251‑145 (MQ=255)                                                                                                                                                      agaattagcctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCt                                                                                                    >  2:157320‑M2/99‑251 (MQ=255)                                                                                                                                                         attagcctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGGGATCATGAAACCGCCGGTTTCGGTCTGcc                                                                                                 >  1:248260‑M2/96‑251 (MQ=255)                                                                                                                                                             tACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatacccttttgaaaagcgccttgaagtcgtgaatcactacttcacaactgatgatggttacaggatcatctcggcacgttttggtgtcccccgaac                                                                                             <  2:184332‑M2/251‑158 (MQ=255)                                                                                                                                                               ctgaaattaaaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTAAGCTCGGTAGCGCCAGGCAGCGGGGTGAACATGAAACCGCCGGTTTCGGTCTGCCACCAgg                                                                                           >  2:246558‑M2/90‑251 (MQ=255)                                                                                                                                                               cATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatacccttttgaaaagcgccttgaagtcgtgaatcactacttcacaactgatgatggttacaggatcatctcggcacgttttggtgtcccccgaaccc                                                                                           <  1:107339‑M2/251‑160 (MQ=255)                                                                                                                                                                        aaaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaaatttttggggccagtacaTTACCAGGCTACCTTCCGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCCccaggtagcgccc                                                                                   >  2:169246‑M2/81‑244 (MQ=255)                                                                                                                                                                         aaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGCCCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAAccgccg                                                                                                              <  1:47507‑M2/143‑1 (MQ=255)                                                                                                                                                                         aaggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAAccgccg                                                                                                              >  2:47507‑M2/80‑222 (MQ=255)                                                                                                                                                                           ggtctgactccaattgaatatcggaatcagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGCACCGGCTTTCAGCTCGGTACCGCCAGGCAGCGGGGTGATCATGAACCCGCCGGTTTCGGTCTGCCACCAGGTATCGaccacc                                                                                >  2:110030‑M2/78‑250 (MQ=255)                                                                                                                                                                              ggAGAAGTAGGTCTGATCAAAACGTTCGTGATCTCCAAACCGCGTACGCGCCTGAACCGGACAGGAGTCGGTGATTAtgtactgaacccagtttgttggacgatgaaatggaatagcccctaatatggcaaagccaaaatacccttttgaaaagcgccttgaagtcgtgagtcacaacttcacaactgatgatggttacaggatcatctcggcacgttttggtgtccccagaacccaggtccggccatggg                                                                            <  2:61673‑M2/251‑175 (MQ=255)                                                                                                                                                                                agaAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatacccttttgaaaagcgccttgaagtcgtgaatcactacttcacaactgatgatggttacaggatcatctcggcacgttttggtgtcccccgaacccaggtcaggacatgggtt                                                                          <  1:18445‑M2/251‑177 (MQ=255)                                                                                                                                                                                      aGGTCTGTTCAAAACTTTCGTGATCGCCATTCAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatacccttttgaaaagcgccttgaagtcgtgaatcactacttcacaactgatgatggttacaggatcatctcggcacgttttggtgtcccccgaacccaggtcaggacatgggttgccct                                                                     >  1:3758‑M2/1‑69 (MQ=255)                                                                                                                                                                                        gTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatacccttttgaaaagcgccttgaagtcgtgaatcactacttcacaactgatgatggttacaggatcatctcggcacgttttggtgtcccccgaacccaggtcaggacatgggttgccctcta                                                                  >  1:62526‑M2/1‑67 (MQ=255)                                                                                                                                                                                            gTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatacccttttgaaaagcgccttgaagtcgtgaatcactacttcacaactgatgatggttacaggatcatctcggcacgttttggtgtcccccgaacccaggtcaggacattggttgccctctatga                                                               >  2:154899‑M2/1‑63 (MQ=255)                                                                                                                                                                                                      cagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGCACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTcc                                                    >  2:150570‑M2/51‑251 (MQ=255)                                                                                                                                                                                                      cagacctatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGCCATTTCTCGTTGCCGATTTTTTTcc                                                    >  2:117979‑M2/51‑251 (MQ=255)                                                                                                                                                                                                          tGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatacccttttgaaaagcgccttgaagtcgtgaatcactacttcacaactgatgatggttacaggatcatctcggcacgttttggtgtcccccgaacccaggtcaggacatgggttgccctctatgaaaaacctggagaaa                                                 <  2:172852‑M2/250‑202 (MQ=255)                                                                                                                                                                                                           gATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatacccttttgaaaagcgccttgaagtcgtgaatcactacttcacaactgatgatggttacaggatcatctcggcacgttttggtgtcccccgaacccaggtcaggacatgggttgccctctatgaaaaacatggagaaaa                                                >  2:43408‑M2/1‑48 (MQ=255)                                                                                                                                                                                                            tatatgcctcgtttttaactgtccatctttttggggtaagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTTTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCg                                                                                      <  1:147069‑M2/167‑1 (MQ=255)                                                                                                                                                                                                            tatatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGGGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCg                                                                                      >  2:147069‑M2/45‑211 (MQ=255)                                                                                                                                                                                                             atatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTAcc                                              >  2:136531‑M2/44‑250 (MQ=255)                                                                                                                                                                                                             atatgcctcgtgtttaactgtccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTAcc                                              >  1:231442‑M2/44‑250 (MQ=255)                                                                                                                                                                                                                  aaaCAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatacccttttgaaaagcgccttgaagtcgtgaatcactacttcacaactgatgatggttacaggatcatctcggcacgttttggtgtcccccgcacccaggtcaggacatgggttgccctctatgaaaaacatggagaaaaaggttta                                         <  1:167516‑M2/250‑210 (MQ=255)                                                                                                                                                                                                                  aaaCAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatacccttttgaaaagcgccttgaagtcgtgaatcactacttcacaactgatgatggttacaggatcatctcggcacgttttggtgtcccccgaacccaggtcaggacatgggttgccctcta                                                                  >  1:111066‑M2/1‑41 (MQ=255)                                                                                                                                                                                                                  aaaCAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatacccttttgaaaagcgccttgaagtcgtgaatcactacttcacaactgatgatggttacaggatcatctcggcacgttttggtgtcccccgaacccaggtcaggacatgggttgccctcta                                                                  <  2:111066‑M2/225‑185 (MQ=255)                                                                                                                                                                                                                      aGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatacccttttgaaaagcgccttgaagtcgtgaatcactacttcacaactgatgatggttacaggatcatctcggcacgttttggtgtcccccgaacccaggtcaggacatgggttgccctctatgaaaaacatggagaaaaaggtttaattcc                                    <  1:208316‑M2/251‑215 (MQ=255)                                                                                                                                                                                                                      aGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatacccttttgaaaagcgccttgaagtcgtgaatcactacttcacaactgatgatggttacaggatcatctcggcacgttttggtgtcccccgaacccaggtcaggacatggggtgccctctatgaaaaacatggagaaaaaggtgtaattcc                                    >  2:137030‑M2/1‑37 (MQ=255)                                                                                                                                                                                                                        gtttaactgtcccactttttggggccagtacaTTACCAGGCTACCTTCGGAGGCCCCCTCCAGCGGGCTACATTCGTTTTCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGACGCACCGGCTTTCAGCTCGGTAGCGCACGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGAATCGACCACCGGACATTTCTCGTTGCCGCTTTTTTTCCCGTACCAATCCCACGCtt                                  >  2:29598‑M2/33‑251 (MQ=255)                                                                                                                                                                                                                         gTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatacccttttgcaaagcgccttgaagtcgtgaatcactacttcacaactgatgatggttacaggatcatctcggcacgttttggtgtcccccgaacccaggtcagtacatgggttgccctctatgaaaaacatggagaaaaaggattaattccca                                  <  2:3226‑M2/250‑217 (MQ=255)                                                                                                                                                                                                                           taactgtacaactttttggggtcaatacaTTACCAGGCTACCTTCGGTGGCCCACTCCAACGGGTTACCTTCGTTCTAGACCAGCGCCGTTTGCACGCCGAAGAACGAACGTGTTGCCGACCCTGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAACCCGCCGGTTTCGGTCTGCCACCAGACATCGACCACCGGACATTTCTCGTTCACGATTTTTTTCCCGTACCACTCCCACGCTtccc                               >  2:278852‑M2/30‑250 (MQ=255)                                                                                                                                                                                                                            gtccccatacccctccatcagtcggttattatggactgcccccatttttatggacgatgaaatggaatagccccttatatgtcaaagccaaaatacccttttgaaaagcgccttgaagtcgtgaatcactactgcacaactgatgatggtttcaggatcatctcggcacggtttggtgtcccccgaaaccaggtcaggacatgggttgccctctatgaaaaacatggagaaaaaggtttaattcccaaac                               >  2:102819‑M2/20‑31 (MQ=255)                                                                                                                                                                                                                                 tccaactttttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTa                          >  2:169778‑M2/24‑250 (MQ=255)                                                                                                                                                                                                                                    cccgttttggggtcattacaTCACCAGGCTCCCTTCGGTGGCCCCCGCCAGCGGGTTACCTGCGTTATGGCCCAGCGCGGGTTGCACGCCGAAGAACGGACGTGTGGCCGACCCGGCTTGCAGCGCGTTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTg                      <  2:125616‑M2/231‑1 (MQ=255)                                                                                                                                                                                                                                         tttggggtcagtacaTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCg                 >  1:68844‑M2/16‑251 (MQ=255)                                                                                                                                                                                                                                           cAGGAGTCGGGGATTAtgtactgcacccattttgttggacgatgaaatggaatagcccctaatatgtcaaagccaaaatacccttttgaaaagcgccttgaagtcgtgaatcactacttcacaactgatgatggttacaggatcatctcggcacgttttggtgtcccccgaacccaggtcaggacatgggttgccctctatgaaaaacatggagaaaaaggtttaattcccaaacctaaaggcgttagtgc               >  2:217581‑M2/1‑16 (MQ=255)                                                                                                                                                                                                                                            ggggtcagtacaTTACCAGGCTACCTTCGGTGGCCACCTCCAGCGGGTCCCCTTCCATATCGACCAGCGCCGGTTGCACGGCGACGACCGGCAGTGCTGCCGACCCGGCTTTAAGCTCGGTAGCGACAGGCAGCGCGGTGATCATGAAAACGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGCCATTTCTCGTTGCCGATTTTTTTCCCGTACCACTCCCACGCTTCCGGGATAATTGGCTCGccc              >  1:274297/12‑251 (MQ=255)                                                                                                                                                                                                                                                  agtacaGTACCAGGCTACCTTCGGTGGCCCCCCCCAGGGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTAAGCTGGGTAGCGCCGGGCGGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGaa        <  2:54959‑M2/245‑1 (MQ=255)                                                                                                                                                                                                                                                       aTTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTCCCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCg     >  2:143896/1‑249 (MQ=255)                                                                                                                                                                                                                                                        ttACCAGCCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCCCCGTTATCAACCAGCGCCGGTTGCACGACGAACAAAGGACGTGTTGCCGAACCGGCTTTCAGATCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACTTTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCCCGCCTCCGGATTAATTGGCTCGCCCACGGAACCg     >  2:233482/1‑248 (MQ=255)                                                                                                                                                                                                                                                         tACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCgaga  >  1:102090/1‑250 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                         ACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGA  >  NC_000913/4285729‑4286226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.