Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 2,913,392 IS2 (–) +5 bp coding (256‑260/2235 nt) relA ← GDP/GTP pyrophosphokinase

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 2913392 =0 (0.000)11 (0.410) 11/490 0.9 100% coding (260/2235 nt) relA GDP/GTP pyrophosphokinase
?NC_000913 = 2997691 NA (NA)noncoding (1/1331 nt) IS2 repeat region
* ? NC_000913 = 29133960 (0.000)19 (0.720) 18/490 0.4 100% coding (256/2235 nt) relA GDP/GTP pyrophosphokinase
?NC_000913 2996361 = NA (NA)noncoding (1331/1331 nt) IS2 repeat region

GGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTCCAGATAACCTGGGGATTCTCCCCCTAAATCAAGCTGTAACGTTATTCTTTCATAACCTTTATCTATGAGGTGGTTCAACCTCGTTGTTGAAAGAGTTTCATCCAGTTCATTGACTTTAGCTTCGCGT  >  NC_000913/2997559‑2997917
                                                                                                                                    |                                                                                                                                                                                                                                  
ggTACGCCGGCTTCGGTTCTCCGGCCCTAAGACATCAACCATCTGATCTCCAATGACGAGTCTAAAAACGAGTATTAAGACTATCACTTATTTAAGTGATATGTGTTGTCTGGAGATGCAGGGGGGCAGTCTAAACTTGCGCTGGTCTTCTGGTGATTAGCTTTGAAAGATGGGTAGGATAAATATCACCAAGTAAAGCCGCGGTGGTGTCTCCGTAATGCGTAACTTGATCCAGATAACCAGGGGATtct                                                                                                              <  2:75200/251‑1 (MQ=9)
                   ttcGGCCCTAAAACCTCAATCCTCTGTTCTCCAATGACTAGACTAAGAACTAGTATTAAGACTATCACTTATTTCAGCGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTCCAGATAACCTGGGGATTCTCCCACTCAATCAAGCTGt                                                                                            >  2:33074/3‑250 (MQ=37)
                                                                                  aTCACTTATTTACGTGATATTGGTTGTCTGGCGATTCAGGGGGCCAGTCTACACTTGATCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAACGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTCCAGATAACCTGGGGCTTCTACCCCTAAATCAAGCTGTAACGTTATTCTTTCATAACCTTTATCTATGAGGTGGTTCAACCTCGTTGTTGAACGAGTTTCAt                            >  2:163104/1‑251 (MQ=255)
                                                                                                            tCTGGAGATTCAGGGGGCCAGTCTAAACTTGCTCTTTTATTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTCCAGATAACCTGGGGATTCTCCCCCTAAATCAAGCTGTAACGTTATTCTTTCATAACCTTTATCTATGAGGTGGTTCAACCTCGTTGTTGAAAGAGTTTCATCCAGTTCATTGACTTTAGCTTCGCgt  >  1:112392/1‑251 (MQ=255)
                                                                                                                                    |                                                                                                                                                                                                                                  
GGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTCCAGATAACCTGGGGATTCTCCCCCTAAATCAAGCTGTAACGTTATTCTTTCATAACCTTTATCTATGAGGTGGTTCAACCTCGTTGTTGAAAGAGTTTCATCCAGTTCATTGACTTTAGCTTCGCGT  >  NC_000913/2997559‑2997917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.