Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,931,171	IS4 (–) +12 bp	intergenic (‑78/‑134)	ravA ← / → kup	regulatory ATPase RavA/K(+):H(+) symporter Kup

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3931171 =	0 (0.000)	24 (0.900)	23/490	0.3	100%	intergenic (‑78/‑145)	ravA/kup	regulatory ATPase RavA/K(+):H(+) symporter Kup
	?	NC_000913	= 4503515	NA (NA)					noncoding (1/1426 nt)	IS4	repeat region
*	?	NC_000913	= 3931182	0 (0.000)	16 (0.610)	15/488	0.6	100%	intergenic (‑89/‑134)	ravA/kup	regulatory ATPase RavA/K(+):H(+) symporter Kup
	?	NC_000913	4502090 =	NA (NA)					noncoding (1426/1426 nt)	IS4	repeat region

TCACGAAGTGTATATAACGGGCTGGTACCAATATCGCCGTAGACAACTCCAATCGCCGCGAGGGTAATCGCGGGCAATGATTGCTTATTATCAGTGCTCATAGACTAGTCTTTCGTTGAAATATGAAATGTGTGCTTAGTCCCTTGGCCCACAAAAAAGCGCACAGTATGCACGATTAACGGCAAAATCGTACTCCTAAATGCGGCCACATTAACCTGGCTCAAAGAAAAATACCCCCCCTTTGAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3931416‑3931171 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTTACGATTCTCTGCGTAACCCACTGACTTCTCTGGGGGATTACCTCGACCCCGAACTCATCTCTCGTTGCCTTGCCGAATCAGGTACTGTAACGCTACGCAAGCGCCGTCTTCCCCT  <  NC_000913/4503515‑4503280                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    TCACGAAGTGTATATAACGGGCTGGTACCAATATCGCCGTAGACAACTCCAATCGCCGCGAGGGTAATCGCGGGCAATGATTGCTTATTATCAGTGCTCATAGACTAGTCTTTCGTTGAAATATGAAATGTGTGCTTAGTCCCTTGGCCCACAAAAAAGCGCACAGTATGCTCGATTAACGGCAAAATCGTACTCCTAAATGCGGCCGCATTATCCTGGCTCAAAGAAAAATACCCCCCCTTTGAGTAAT                                                                                                                                                                                                                                          <  1:241894/250‑1                    GGCTGGTACTAATAGCGCCGTAGACAACGCAAGTCGCCGCGAGGGTAATCGCGGACAATGATTGCTTAATAGCAGTGCTCATAGCCTAGTCTTTCGTTGAAATATGAAATGTGTGCTTAGGCCCTTGGCCCACAAAAAAGCGCACAGTATTCACGATTAACGGCAAAATCGTACGCCTAAATGCGGCCGCATTAACCTGGCTGAAAGAAAAATACACCCCCTTTGAGTAATGCCGATCAGTTAAGGATCAG                                                                                                                                                                                                                      <  2:22133/251‑1                            CCAATATCGCCGTAGACAACCCCAATCGCCGCGAGGGTAATCGCGGGCAATGATTGCTTAATATCAGTGCTCATAGACTAGTCTTTCGTTGAAATATGAAATGAGTGCTTAGGCCCTTTGCCCACAAAAAAGCGCACAGTATGCACGATTAACGGCTAAATCGTACTCCTAAATGCGGCCACATTAACCTGACTCAAAGAAAAATACCCCCCCTTTGAGTAATGCCGATCAGTTAAGGATCAGTTGACCGA                                                                                                                                                                                                              <  1:248778/251‑1                                                    ATCGCCGCGAGGGTAATCGCGGGCAATGATTGCCTATTATCAGTGCTCATAGACTAGTCTTTCGTTGAAATATGAAATGTGTGCTTAGTCCCTTGGCCCACAAAAAAGCGCACAGTATGCACGATTAACGGCAAAATCGTACTCCTAAATGCGGCCACATTAACCTGGCTCAAAGAAAAATACCCCCCCTTTGAGAAAAGCCGATCAGTTAAGGATCAGTAGACCGATCCAGTGCATGTGTAAGAAACCGG                                                                                                                                                                                      >  1:112610/1‑251                                                            GAGGGTAATCGCGGGCAATGATTGCTTATTATCAGTGCTCATAGACTAGTCTTTCGTTGAAATATGAAATGTGTGCTTAGTCCCTTGGCCCACAAAAAAGCGCACAGTATGCACGATTAACGGCAAAATCGTACTCCTAAATGCGGCCACATTAACCTGGCTCAAAGAAAAATACCCCCCCTTTGAGTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTC                                                                                                                                                                              <  1:237172/251‑1                                                                GTAATCGCGGGCACTGATTGCTTATTATCAGTGCTCATAGCCTAGTCTTTCGTTGAAATATGAAATGTGTGCTTAGTCCCTTGGCCCACAAAAAAGCGCACAGTATGCACGATTACCGGCAAAATAGTACTCCTAAATGCGGCCACATTAACCTGGCTCAAAGAAAAATACCCCCCCTTTGAGTAATGCCGAGCAGTCACGGATCAGTTGACCGATCCAGAGGCTGTGAAAGAAGCCGGCAATGCTCACTT                                                                                                                                                                          >  1:119284/1‑251                                                                          GCAATGATTGCTTATTATCAGTGCTCATAGACTAGTCTTTCGTTGAAATATGAAATGTGTGCTTAGTCCCTTGGCCCACAAAAAAGCGCACAGTATGCACGATTAACGGCAAAATCGTACTCCTAAATGCGGCCACATTAACCTGGCTCAAAGAAAAATACCCCCCCTTTGAGTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAAAGCTCACTTGTTTCCGGAT                                                                                                                                                                >  2:135104/1‑251                                                                                                    ATAGACTAGTCTTTCGTTGTAATATTAAATTTGGGCTTAGTCCCTTGGCCCACAAAAAAGCGCACAGTATGCACGATTAACGGCAAAATCGTACTCCGAAATGCGGCCACATTAACCTGGCTCAAAGAAAAATACCCCCCCTTTGAGTAATGCCGATCAGTTAAGGATCAGTTGACCGAGCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGTTCT                                                                                                                                      <  2:159750/251‑1                                                                                                      AGACTAGTCTTTCGTTGAAATATGAAATGTGTGCTTAGTCCCTTGGCCCACAAAAAAGCGCACAGTATGCACGATTAACGGCAAAATCGTACTCCTAAATGCGGCCACATTAACCTGGCTCAAAGAAAAATACCCCCCCTTTGAGTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTG                                                                                                                                    >  1:228425/1‑251                                                                                                                  CGTTGAAATATGAAATGTGTGCTTAGTCCCTTGGCCCACAAAAAAGCGCACAGTATGCACGATTAACGGCAAAATCGTACTCCTAAATGCGGCCACATTAACCTGGCTCAAAGAAAAATACCCCCCCTTTGAGTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCC                                                                                                                        >  1:94267/1‑251                                                                                                                      GAAATATGAAATGTGTGCTTAGTCCCTTGGCCCACAAAAAAGCGCACAGTATGCACGATTAACGGCAAAATCGTACTCCTAAATGCGGCCACATTAACCTGGCTCAAAGAAAAATACCCCCCCTTTGAGTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTT                                                                                                                     <  1:107264/250‑1                                                                                                                                 TGTGGGCTTAGTCCCTTAGCCCACAAAAAAGCGCACAGTATGCACGATTAACGGCAAAATCGTACTCCTAAATGCGCCCACATTAACCTGGCTCAACGAAAAATACCCCCCCTTTGAGTAATGACGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCCATTGTTTCCGGATTTTTTTATGCCCATTGGACCGGCTCTTGATCTGGTATCCCGTTACGATTATCTGg                                                                                                         >  2:89456/1‑250                                                                                                                                                                                        AAAATCGTACTCCTAAATGCGGCCACCTTACCCTGGCTCAAAGAAAAATACACCCCCTTTGAGTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGCGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTTACGATTCTCTGCGTACCCCTCTTACTTCTCTGGGGGATTCCTTCGACCCCGCACTCATCTCcctttg                                                  >  2:29991/1‑245                                                                                                                                                                                           ATCGTACTCCTAAATGCGGCCACATTAACCTGGCTCAAAGAAAAATACCCCCCCTTTGAGTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTTACGATTCTCTGCGTAACCCACTGACTTCTCTGGGGGATTACCTCGACCCCGAACTCATCTCTCGTTGCCT                                               >  2:157059/1‑251                                                                                                                                                                                                  TCCTAAATGCGGCCACATTAACCTGGCTCAAAGAAAAATACCCCCCCTTTGAGTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTTACGATTCTCTGCGTAACCCACTGACTTCTCTGGGGGATTACCTCGACCCCGAACTCATCTCTCGTTGCCTTGCCGA                                         >  1:56421/1‑250                                                                                                                                                                                                  TCCTAAATGCGGCCACATTAACCTGGCTCAAAGAAAAATACCCCCCCTTTGAGTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTTACGATTCTCTGCGTAACCCACTGACTTCTCTGGGGGATTACCTCGACCCCGAACTCATCTCTCGTTGCCTTGCCGA                                         >  1:164178/1‑250                                                                                                                                                                                                     TAAATGCGGCCACATTAACCTGGCTCAAAGAAAAATACCCCCACTTTGAGTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTCTGCACATTGGGCAGGCTCTTGATCTGGTATCCCGTTACGCTTCTCTGCGTAACCCACTGACTTCTCTGGGGGATTACCTCGACCCCGACCTCATCTCTCGTTGCCTTGCCGAATC                                      >  2:253285/1‑250                                                                                                                                                                                                              CCACATCAACCTGGCTCAAAGAGAAATACCCCCCCTTTGAGTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTTACGATTCTCTGCGTAACCCACTGACTTCTCTGGGGGATTACCTCGACCCCGAACTCATCTCTCGTTGCCTTGCCGAATCAGGTACTGT                             >  1:74974/1‑250                                                                                                                                                                                                               CACATTAACCTGGCTCAAAGAAAAATACCCCCCCTTTGAGTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTTACGATTCTCTGCGTAACCCACTGACTTCTCTGGGGGATTACCTCGACCCCGAACTCATCTCTCGTTGCCTTGCCGAATCAGGTACTGTAA                           <  2:94267/251‑1                                                                                                                                                                                                                     AACCTGGCTCAAAGAAAAATACCCCCCCTTTGAGTAATGACGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATACGGAAACGCTCACTTGATTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTTACGATTCTCTGCGTAACCCACTGACTTCTCTGGGGGATTACCTCGACCCCGAACTCATCTCTCGTTGCCTTGCCGAATCAGGTACTGTAACGCTAC                     >  1:253565/1‑251                                                                                                                                                                                                                       CCTGGCTCAAAGAAAAATACCCCCCCTTTGAGTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCGCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTTACGATTCTCTGCGTAACCCACTGACTTCTCTGGGGGATTACCTCGACCCCGAACTCATCTCTCGTTGCCTTGCCGAATCAGGTACTGTAACGCTACGC                   <  2:205133/251‑1                                                                                                                                                                                                                              CAAAGAAAAATACCCCCCCTTTGAGTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTTACGATTCTCTGCGTAACCCACTGACTTCTCTGGTGGATTACCTCGACCCCGAACTCATCTCTCGTTGCCTTGCCGAATCAGGTACTGTAACGCTACGCAAGCGCC            <  1:106574/251‑1                                                                                                                                                                                                                                 AGAAAAATACCCCCCCGTTGAGTAATGCCGATCAGTTAAGGATCAGTGGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTGCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTTACGATTCTCTGCGTAACCCACTGTCTTCTCTGGGGTATTACCTCGACCCCGAGCTCATCTCTCGTTGCCTTGTCGAATCAGGTACTGTAACGCTACGCAATCGCCGTC         <  2:21066/251‑1                                                                                                                                                                                                                                         gccaCCCCTGTGAGTAATGCCGATCAGTTAAGGATCAGATGCACGATCCAGTGGTAGTGTAACAAGCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCCCACTGGACAGGCTCTTGATCTGGTATCCCGGTACGATTCTCTTAGTAACCCACTGACTTCTCTGGGGGATTACCTCGACCCCGAACTCATCGCTCGTTGCCTTGCCGAATCATGTACTGTAACGCTACGCAAGCGCCGTCTTCCCCT  <  2:144554/246‑1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    TCACGAAGTGTATATAACGGGCTGGTACCAATATCGCCGTAGACAACTCCAATCGCCGCGAGGGTAATCGCGGGCAATGATTGCTTATTATCAGTGCTCATAGACTAGTCTTTCGTTGAAATATGAAATGTGTGCTTAGTCCCTTGGCCCACAAAAAAGCGCACAGTATGCACGATTAACGGCAAAATCGTACTCCTAAATGCGGCCACATTAACCTGGCTCAAAGAAAAATACCCCCCCTTTGAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3931416‑3931171 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTCACTTGTTTCCGGATTTTTTTATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTTACGATTCTCTGCGTAACCCACTGACTTCTCTGGGGGATTACCTCGACCCCGAACTCATCTCTCGTTGCCTTGCCGAATCAGGTACTGTAACGCTACGCAAGCGCCGTCTTCCCCT  <  NC_000913/4503515‑4503280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.