Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	2,942,765	Δ8 bp	noncoding (70‑77/206 nt)	gcvB →	small regulatory RNA GcvB

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 2942764	2 (0.050)	23 (0.580)	21/478	0.7	92.1%	noncoding (69/206 nt)	gcvB	small regulatory RNA GcvB
	?	NC_000913	2942773 =	2 (0.050)					noncoding (78/206 nt)	gcvB	small regulatory RNA GcvB

GTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCGAATTACTGATCCAGTTCGACCATCTCTTTCACGTCAGTACGGTTGATCTGCTGTTTGTTGCCATTAGCGTCTTTATACGAAATCATACCGGTATCGTTATCAGTCTGT  >  NC_000913/2942534‑2943023                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                           aaaaactcgtaaggcatttagcggtggtaatcgtttagacatggctattaaactttgatgttaaatgaatttaacaattagatcacactatgtaacctattagtttttttaatctgagccattataaattgtccgttgagcttctaccagcaaatacctatagtggcggcacttcctgagtcggaacgaaaagttttatctgaatgcgtgttctggtgaacttttggcttacggttgtgTTGTGGTGTTTg                                                                                                                                                                                                                                                 <  1:165025‑M2/12‑1 (MQ=255)   aaGGCACTTAGAGGTGGAAATCGCTTAGACATGACTATTAAAATTTGATGTTAAATGACTTTAACAATTTGATCACAATATGTAACCTATTAGTTTTTTTAATATGAACCATTATAAATTGTCCGCTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTTCTAAGCCGAAAAGAAACGTTTTATCGGCATGCATGTTTTGGCGAACTTTTGGCTTACGGTTGTGTttttgtgtttgccattggtct                                                                                                                                                                                                                                               >  2:395079/1‑239 (MQ=255)                               ccgtatagacatggctattaaactttgatggtaaatgaatttaacaattagataacactatgtatcctattagtgtttttaatcggagccattataaattgtcctttgagcttctaccagcaaatacctatagtggcggcacttcctgagccggaactaaaagttttatcggaatgcgggttctggtgaacttttggcttacggttgtgGTGTGGTGTTTGCAAGTGGTCTGCGATTCAGACCATGTTAGc                                                                                                                                                                                                                   <  2:297330‑M2/42‑1 (MQ=255)                                        catggctattaaactttgatgttaaatgaatttaacaattagatcacactatgtaccctattagtttttttaatctgagccattataaattgtccgttgagcttctaccagcaaatacctatagtggcggcacttcctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgTTGTTGTGTTTGCACTTGGTCTGCGATTCAGACCATGGTAGCAAAGCTAcc                                                                                                                                                                                                          >  1:276165‑M2/201‑251 (MQ=255)                                               attatactttgaggttaaatgaattgaacaattagatcacactatgtaccttattagtttttttaatctgagccattataaattgtccgttgagcttctaccagcaaatacctatagtggcggcacttcctgagccgtaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgTTCTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCtttttt                                                                                                                                                                                                    <  1:4371‑M2/57‑1 (MQ=255)                                               attaaactttgatgttaaatgaatttaacaattagatcacactatgtaacctattagtttttttaatctgagccattataaattgtccgttgagcttctaccagcaaatacctatagtggcggaacttcctgagacggaacgaaaagttttatcggaatgcgtgttctggtaaacttttggcttacggttgtgTTGTTGTGTTTGCAATTGGTCTGCGATTAAGACCATGGTAGCGAAGCTACCTTTTTTc                                                                                                                                                                                                   >  2:123454‑M2/194‑251 (MQ=255)                                                ttaaactttgatgttaaatgaatttaacaattagatcacactatgtaacctattagtttttttaatctgagccattataaattgtccgttgagcttctaccagcaaatacctatagtggcggcacttcctgagccggaacgaaaagttttatcggcatgcgtgttctggtgaacttttggcttacggttgtgTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTc                                                                                                                                                                                                   >  2:377940‑M2/193‑250 (MQ=255)                                                                          acaattagatcacactatgtaacctattagtttttttaatctgagccattataaattgtccgttgagcttctaccagcaaatacctatagtggcggcacttcctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCtgtctgtc                                                                                                                                                                        >  1:5301‑M2/167‑251 (MQ=255)                                                                                agatcacactatgtaacctattagtttttttaatctgagccattataaattgtccgttgagcttctaccagcaaatacctatagtggcggcacttcctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAg                                                                                                                                                                   <  1:323048‑M2/90‑1 (MQ=255)                                                                                  atcacactatgtaacctattagtttttttaatctgagccattataaattgtccgttgagcttctaccatcaaatacctatagtggcggcacttcctgagcaggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATTGTAGCAAAGCTACCTTTTTTCTCTTTCTGTACATTTACCCTGTCTGTCCAtaggg                                                                                                                                                                 >  2:228761‑M2/159‑248 (MQ=255)                                                                                         tatgtaacctattagtttttttaatctgagccattataaattgtccgttgagcttctaccagcaaatacctatagtggcggcacttcctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGt                                                                                                                                                         >  1:74991‑M2/152‑251 (MQ=255)                                                                                                   attagtttttttaatctgagccattataaattgtccgttgagcttctaccagcaaatacctatagtggcggcacttcctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaactgttggcttacggttgtgTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTTCCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCt                                                                                                                                               <  2:88037‑M2/110‑1 (MQ=255)                                                                                                              taatctgagccattataaattgtccgttgagcttctaccagcaaatacctatagtggcggcacttcctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTg                                                                                                                                     <  2:74991‑M2/120‑1 (MQ=255)                                                                                                                          ttataaattgtccgttgagcttctaccagcaaatacctatagtggcggcacttcctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggattacggttgtgTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTATCACCGCCTAATTGCGGTGCTTTTTTTTACCt                                                                                                                        >  2:312437‑M2/119‑251 (MQ=255)                                                                                                                            ataaattgtccgttgagcttctaccagcaaatacctatagtggcggcacttcctgagccggaacgaaaaggtttatcgtaatgcgttttctggggaacttttggcgtacggtggtgTTGTTGTGTTGGCAATTGTTCTTCGAGTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCGGGCCATTTACCCTGTCCGTCCATAGTGATTAATTTATCACCGCCTAATTGCGGTGCTTTTTTTTACCTTg                                                                                                                      <  1:407139‑M2/135‑1 (MQ=255)                                                                                                                                       gttgagcttctaccagcaaatacctatagtggcggcacttcctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCGAAt                                                                                                           <  1:58806‑M2/146‑1 (MQ=255)                                                                                                                                                         aatacctatagtggcggcacttcctgagccggaacgaaaagttttatcggaatgcgggttttggtgaacttttggcttacgggtgtgTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACTTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCGAATTACTGATCCAGTTCGAcc                                                                                         <  1:197933‑M2/164‑1 (MQ=255)                                                                                                                                                             cctgtagtggcggcccttcctgaaccggaacgaaaagttatatcggaatgcgtgttcttgtgaactgttggcttacggttgtgGTGTTGTGTTTGCAATTGGTATGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTTCGATAGCGAATTACTGATCCAGGTCGACCAtct                                                                                     >  2:125882‑M2/84‑251 (MQ=255)                                                                                                                                                                   gtggcggcacttcctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCGAATTACTGATCCAGTTCGACCATCTCTTTCa                                                                               <  1:188597‑M2/174‑1 (MQ=255)                                                                                                                                                                                       ggaacgcaaagatttatcggaatgcgtgttctggtgaacttttggcttacggttgtgTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCGAATTACTGATCCAGTTCGACCATCTCTTTCACGTCAGTACGGTTGATctgc                                                           >  2:45542‑M2/58‑251 (MQ=255)                                                                                                                                                                                           cgtaaatttttttcggaatgcgtgttctggtgaactttttgcttacggttgttTTTTTGTGTTTTCCATTTGTTTGCGATTCAGACCATGGTATCAAAGCTACTTTTTTTCACTTCCTTTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCTCCGCCTAATTGCGGTTCTTTTTTTTACCTTGCGATCGCGAATTACTGATCCAGTTCGACCATCTCTTTCCCTTCAGTACGGTTGATCTGCtgtt                                                       <  2:113914‑M2/198‑1 (MQ=255)                                                                                                                                                                                           cgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCGAATTACTGATCCAGTTCGACCATCTCTTTCACGTCAGTACGGTTGATCTGCtgtt                                                       <  1:377940‑M2/198‑1 (MQ=255)                                                                                                                                                                                             tATCGGAATGAGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGGTGTTGTGTTGTGGTGTTTGCAATTTGTCTGTGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCATCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCGAATTACTGATCCAGTTCGACCATCTCTTTCACGTCAGTACGGTTGATCTGCtgttt                                                      <  2:126482/250‑1 (MQ=255)                                                                                                                                                                                                     tatcggaatgcgtgttctggtggacttttggcttacggttgtgTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCGAATTACTGATCCAGTTCGACCATCTCTTTCACGTCAGTACGGTTGATCTGCTGTTTGTTGCCAt                                              <  1:38685‑M2/207‑1 (MQ=255)                                                                                                                                                                                                                           aacttttggcttacggttgtgTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCCTGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCGAATTACTGATCCAGTTCGACCATCTCTTTCACGTCAGTACGGTTGATCTGCTGTTTGTTGCCATTAGCGTCTTTATACGAAATCAt                        >  2:265842‑M2/22‑250 (MQ=255)                                                                                                                                                                                                                                           ttgtgttgttgagttTGCAATTGGTCTGCGCTTCAGCCCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCGACTTACTGATCCAGTTCGACCATCTCTTTCACGTCAGTACGGTTGATCTGCTGTTTGTTGCCATTAGCGTCTTTATACGAAATCATACCGGTATCGTTATc         >  2:52409/1‑249 (MQ=255)                                                                                                                                                                                                                                                ttgttgtgttTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCGAATTACTGATCCAGTTCGACCATCTCTTTCACGTCAGTACGGTTGATCTGCTGTTTGTTGCCATTAGCGTCTTTATACGAAATCATACCGGTATCGTTATCAGTCtgt  >  2:150915/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                           GTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCGAATTACTGATCCAGTTCGACCATCTCTTTCACGTCAGTACGGTTGATCTGCTGTTTGTTGCCATTAGCGTCTTTATACGAAATCATACCGGTATCGTTATCAGTCTGT  >  NC_000913/2942534‑2943023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.