Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	2,942,765	Δ8 bp	noncoding (70‑77/206 nt)	gcvB →	small regulatory RNA GcvB

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 2942764	2 (0.050)	23 (0.580)	21/478	0.7	92.1%	noncoding (69/206 nt)	gcvB	small regulatory RNA GcvB
	?	NC_000913	2942773 =	2 (0.050)					noncoding (78/206 nt)	gcvB	small regulatory RNA GcvB

AAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCGAATTACTGATCCAGTTCGACCATCTCTTTCACGTCAGTACGGTTGATCTGCTGTTTGTTGCCATTAGCGTCTTTATAC  >  NC_000913/2942526‑2942993                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                      aaaaaCTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGTCGGAACGAAAAGTTTTATCTGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGttgtggtgtttg                                                                                                                                                                                                                           <  1:165025‑M1/251‑13 (MQ=255)           aaGGCACTTAGAGGTGGAAATCGCTTAGACATGACTATTAAAATTTGATGTTAAATGACTTTAACAATTTGATCACAATATGTAACCTATTAGTTTTTTTAATATGAACCATTATAAATTGTCCGCTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTTCTAAGCCGAAAAGAAACGTTTTATCGGCATGCATGTTTTGGCGAACTTTTGGCTTACGGTTGTGTttttgtgtttgccattggtct                                                                                                                                                                                                                 >  2:395079/1‑239 (MQ=255)                               ccgtatagacaTGGCTATTAAACTTTGATGGTAAATGAATTTAACAATTAGATAACACTATGTATCCTATTAGTGTTTTTAATCGGAGCCATTATAAATTGTCCTTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAACTAAAAGTTTTATCGGAATGCGGGTTCTGGTGAACTTTTGGCTTACGGTTGTGgtgtggtgtttgcaagtggtctgcgattcagaccatgttagc                                                                                                                                                                                             <  2:297330‑M1/246‑43 (MQ=255)                                        cATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTACCCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGttgttgtgtttgcacttggtctgcgattcagaccatggtagcaaagctacc                                                                                                                                                                                    >  1:276165‑M1/1‑200 (MQ=255)                                               aTTATACTTTGAGGTTAAATGAATTGAACAATTAGATCACACTATGTACCTTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGTAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGttcttgtgtttgcaattggtctgcgattcagaccatggtagcaaagctacctttttt                                                                                                                                                                              <  1:4371‑M1/250‑58 (MQ=255)                                               aTTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGAACTTCCTGAGACGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTAAACTTTTGGCTTACGGTTGTGttgttgtgtttgcaattggtctgcgattaagaccatggtagcgaagctaccttttttc                                                                                                                                                                             >  2:123454‑M1/1‑193 (MQ=255)                                                ttAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGCATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGttgttgtgtttgcaattggtctgcgattcagaccatggtagcaaagctaccttttttc                                                                                                                                                                             >  2:377940‑M1/1‑192 (MQ=255)                                                                          aCAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGttgttgtgtttgcaattggtctgcgattcagaccatggtagcaaagctaccttttttcacttcctgtacatttaccctgtctgtc                                                                                                                                                  >  1:5301‑M1/1‑166 (MQ=255)                                                                                aGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGttgttgtgtttgcaattggtctgcgattcagaccatggtagcaaagctaccttttttcacttcctgtacatttaccctgtctgtccatag                                                                                                                                             <  1:323048‑M1/250‑91 (MQ=255)                                                                                  aTCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCATCAAATACCTATAGTGGCGGCACTTCCTGAGCAGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGttgttgtgtttgcaattggtctgcgattcagaccattgtagcaaagctaccttttttctctttctgtacatttaccctgtctgtccataggg                                                                                                                                           >  2:228761‑M1/1‑158 (MQ=255)                                                                                         tATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGttgttgtgtttgcaattggtctgcgattcagaccatggtagcaaagctaccttttttcacttcctgtacatttaccctgtctgtccatagtgattaatgt                                                                                                                                   >  1:74991‑M1/1‑151 (MQ=255)                                                                                                   aTTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTGTTGGCTTACGGTTGTGttgttgtgtttgcaattggtctgcgattcagaccatggtagcaaagcttccttttttcacttcctgtacatttaccctgtctgtccatagtgattaatgtagcaccgcct                                                                                                                         <  2:88037‑M1/251‑111 (MQ=255)                                                                                                              tAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGttgttgtgtttgcaattggtctgcgattcagaccatggtagcaaagctaccttttttcacttcctgtacatttaccctgtctgtccatagtgattaatgtagcaccgcctaattgcggtg                                                                                                               <  2:74991‑M1/250‑121 (MQ=255)                                                                                                                          ttATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGATTACGGTTGTGttgttgtgtttgcaattggtctgcgattcagaccatggtagcaaagctaccttttttcacttcctgtacatttaccctgtctgtccatagtgattaatgtatcaccgcctaattgcggtgcttttttttacct                                                                                                  >  2:312437‑M1/1‑118 (MQ=255)                                                                                                                            ataAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAACGAAAAGGTTTATCGTAATGCGTTTTCTGGGGAACTTTTGGCGTACGGTGGTGttgttgtgttggcaattgttcttcgagtcagaccatggtagcaaagctaccttttttcacttccgggccatttaccctgtccgtccatagtgattaatttatcaccgcctaattgcggtgcttttttttaccttg                                                                                                <  1:407139‑M1/251‑136 (MQ=255)                                                                                                                                       gTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGttgttgtgtttgcaattggtctgcgattcagaccatggtagcaaagctaccttttttcacttcctgtacatttaccctgtctgtccatagtgattaatgtagcaccgcctaattgcggtgcttttttttaccttgcgatcgcgaat                                                                                     <  1:58806‑M1/251‑147 (MQ=255)                                                                                                                                                         aaTACCTATAGTGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGGGTTTTGGTGAACTTTTGGCTTACGGGTGTGttgttgtgtttgcaattggtctgcgattcagaccatggtagcaaagctactttttttcacttcctgtacatttaccctgtctgtccatagtgattaatgtagcaccgcctaattgcggtgcttttttttaccttgcgatcgcgaattactgatccagttcgacc                                                                   <  1:197933‑M1/251‑165 (MQ=255)                                                                                                                                                             cctgtagtggCGGCCCTTCCTGAACCGGAACGAAAAGTTATATCGGAATGCGTGTTCTTGTGAACTGTTGGCTTACGGTTGTGgtgttgtgtttgcaattggtatgcgattcagaccatggtagcaaagctaccttttttcacttcctgtacatttaccctgtctgtccatagtgattaatgtagcaccgcctaattgcggtgcttttttttacctttcgatagcgaattactgatccaggtcgaccatct                                                               >  2:125882‑M1/5‑83 (MQ=255)                                                                                                                                                                   gTGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGttgttgtgtttgcaattggtctgcgattcagaccatggtagcaaagctaccttttttcacttcctgtacatttaccctgtctgtccatagtgattaatgtagcaccgcctaattgcggtgcttttttttaccttgcgatcgcgaattactgatccagttcgaccatctctttca                                                         <  1:188597‑M1/251‑175 (MQ=255)                                                                                                                                                                                       ggAACGCAAAGATTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGttgttgtgtttgcaattggtctgcgattcagaccatggtagcaaagctaccttttttcacttcctgtacatttaccctgtctgtccatagtgattaatgtagcaccgcctaattgcggtgcttttttttaccttgcgatcgcgaattactgatccagttcgaccatctctttcacgtcagtacggttgatctgc                                     >  2:45542‑M1/1‑57 (MQ=255)                                                                                                                                                                                           cgtaaatttttttcggaatgCGTGTTCTGGTGAACTTTTTGCTTACGGTTGTTtttttgtgttttccatttgtttgcgattcagaccatggtatcaaagctactttttttcacttcctttacatttaccctgtctgtccatagtgattaatgtagctccgcctaattgcggttcttttttttaccttgcgatcgcgaattactgatccagttcgaccatctctttcccttcagtacggttgatctgctgtt                                 <  2:113914‑M1/244‑199 (MQ=255)                                                                                                                                                                                           cGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGttgttgtgtttgcaattggtctgcgattcagaccatggtagcaaagctaccttttttcacttcctgtacatttaccctgtctgtccatagtgattaatgtagcaccgcctaattgcggtgcttttttttaccttgcgatcgcgaattactgatccagttcgaccatctctttcacgtcagtacggttgatctgctgtt                                 <  1:377940‑M1/251‑199 (MQ=255)                                                                                                                                                                                                     tATCGGAATGCGTGTTCTGGTGGACTTTTGGCTTACGGTTGTGttgttgtgtttgcaattggtctgcgattcagaccatggtagcaaagctaccttttttcacttcctgtacatttaccctgtctgtccatagtgattaatgtagcaccgcctaattgcggtgcttttttttaccttgcgatcgcgaattactgatccagttcgaccatctctttcacgtcagtacggttgatctgctgtttgttgccat                        <  1:38685‑M1/250‑208 (MQ=255)                                                                                                                                                                                                     tATCGGAATGAGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGGTGTTGTGTTGTGGTGTTTGCAATTTGTCTGTGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCATCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCGAATTACTGATCCAGTTCGACCATCTCTTTCACGTCAGTACGGTTGATCTGCtgttt                        <  2:126482/250‑1 (MQ=255)                                                                                                                                                                                                                           aaCTTTTGGCTTACGGTTGTGttgttgtgtttgcaattggtctgcgattcagaccctggtagcaaagctaccttttttcacttcctgtacatttaccctgtctgtccatagtgattaatgtagcaccgcctaattgcggtgcttttttttaccttgcgatcgcgaattactgatccagttcgaccatctctttcacgtcagtacggttgatctgctgtttgttgccattagcgtctttatacgaaatcat  >  2:265842‑M1/1‑21 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                      AAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCGAATTACTGATCCAGTTCGACCATCTCTTTCACGTCAGTACGGTTGATCTGCTGTTTGTTGCCATTAGCGTCTTTATAC  >  NC_000913/2942526‑2942993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.