Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	458,790	IS186 (+) +6 bp :: Δ1 bp	intergenic (+90/‑93)	clpX → / → lon	ATP‑dependent Clp protease ATP‑binding subunit ClpX/Lon protease

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	15387 =	NA (NA)	33 (0.820)	31/482	0.3	97.0%	noncoding (1/1345 nt)	IS186	repeat region
	?	NC_000913	= 458795	1 (0.030)					intergenic (+95/‑93)	clpX/lon	ATP‑dependent Clp protease ATP‑binding subunit ClpX/Lon protease
*	?	NC_000913	458790 =	1 (0.030)	34 (0.840)	32/484	0.3	97.1%	intergenic (+90/‑98)	clpX/lon	ATP‑dependent Clp protease ATP‑binding subunit ClpX/Lon protease
	?	NC_000913	= 609350	NA (NA)					noncoding (1344/1345 nt)	IS186	repeat region

GCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAA‑TGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGC‑AACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTACGTGAAGTCACTGCATGGGCT  >  NC_000913/15138‑15636                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                             gcgttgtctgagAAACACCGTGAGGCCTGAACGAAAGGCAGAAACAGCTGCTGCACGAGCTGCAAGAAAGCTTCGGTGGCTCAACCGTCGAGCACAACAGCCCGCGCTTAAAGAGCTTCTTTGATGGTGTGAAGAAGGTTTTTGTCGACCTGTCCCGCTAACCTCGCCAAAAGCCTTCCCGTGGGCAGGCCTGGGTGAAAATAGGGTGCGTTTAAGATATGCGAGCGCCTTTAAAGGGGCGGGGATCACTc                                                                                                                                                                                                                                                             <  2:140019/240‑1 (MQ=255)                    ggTAGGCCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTCACccccccaagagcgcacattc                                                                                                                                                                                                                                           >  1:411592/1‑234 (MQ=255)                                               gctgctGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGATCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGAtt                                                                                                                                                                                                              <  1:216975/251‑1 (MQ=255)                                                           gctgcaagaAAGCTTCGGTGGCCCAACCGGCGAACACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGAt                                                                                                                                                                                                  <  1:186371/251‑1 (MQ=255)                                                              gcaagaAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGaa                                                                                                                                                                                               <  1:347703/251‑1 (MQ=255)                                                                    aaGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTActc                                                                                                                                                                                         <  1:225607/251‑1 (MQ=255)                                                                            gTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATa                                                                                                                                                                                 <  1:384031/251‑1 (MQ=255)                                                                               gCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACt                                                                                                                                                                              <  1:108764/251‑1 (MQ=255)                                                                                cccAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTg                                                                                                                                                                             <  1:269167/251‑1 (MQ=255)                                                                                      cGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGAGCACCCCCCAAACAGCTCAATTAAGGGGTTG‑GGGATTTCGCCTGATATGGTTTAACATGCCGGTGAAtttatctcccgcaaaaatgtccgc                                                                                                                                                                       >  1:247479/1‑229 (MQ=255)                                                                                          gAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGTG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAAtt                                                                                                                                                                   <  2:406257/251‑1 (MQ=255)                                                                                                                 gagCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTCAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGCACTCCCCTAAAAGCCCACATAAGGG‑TTGGGGTGGTACGCGTGCTATGATTTTACGTGCCGATGAAATACTACCCCGATAACTTGACCGAAAATCTGGCCCCTATTGGTAAACCCgc                                                                                                                                            >  2:98719/1‑250 (MQ=255)                                                                                                                      tcttTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGccc                                                                                                                                              <  2:372633/244‑1 (MQ=255)                                                                                                                      tcttTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCgaagaa                                                                                                                                        <  1:309134/250‑1 (MQ=255)                                                                                                                                           tttttGACGTCCTGGCCCGCTAACCTCCCCAAAAGCCTGTCCGTGGGCGGGCCTGGGTAAAAATATGGTGCGTTGAAGATATGCGAGCATCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGAGCCGAGGAAGTACTCTCACGATAACTGGTCAGCAAGTCTGGCCCATATGGGTAAGCCCGAAGAACCGGATACTTCGGCACGTAA‑t                                                                                                                  <  2:35897/250‑1 (MQ=21)                                                                                                                                              tggacgacCTGACCCGCTAACCTCCCCAAAAGCCTGCCAGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAA‑TGcc                                                                                                               <  2:369420/248‑1 (MQ=255)                                                                                                                                                gacgacCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTTCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAA‑TGCCggg                                                                                                            <  1:164481/251‑1 (MQ=255)                                                                                                                                                        gACCCGCTAACCCCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCTGGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTTGCCCATATTGGTAAGTCCGTAGACCTGGATACTTCGGCACGTAA‑TGCCGGGGCTCTa                                                                                                      <  1:293475/249‑1 (MQ=21)                                                                                                                                                          cccGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGCAATCCCATAAGAGCTAAATTAAGGG‑GTGGGGGATTTCGCCCGATATGTTTTAACGTGCCGCTGAGTTATACTCACCATAACTGGTCAGCCATTCTGGCCCCTATTGGTAAGCCCGCAAAAATGGATAATTTTGCACGTAA‑TGCCGGGGCCCTaaacc                                                                                                  >  1:98864/1‑248 (MQ=255)                                                                                                                                                           ccGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATGCTTCGGCACGTAA‑TGCCGGGGCTCTAACccg                                                                                                 <  1:178572/251‑1 (MQ=255)                                                                                                                                                                  ccTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAA‑TGCCGGGGCTCTAACCcgccgccgc                                                                                          <  1:359926/251‑1 (MQ=255)                                                                                                                                                                                            ggCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAA‑TGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGC‑AACTCTGCt                                                               <  1:368510/251‑1 (MQ=255)                                                                                                                                                                                                    tAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTCACACCCCTAAAAGCTAACCTAAAGG‑GTGTGGGAGTATGCCTGATATGTGTTTACGTGGCGGTGGAATACTATCACGAAAAAACGGCAGCAATTTTGGCCCATATTGGTAAACCCGAAGAAACGGGTAATTCCGCACGGAA‑AGGCGGGGGTCTGACCCGCCGCCCCGCAAATCGTGATGGCGCGAAC‑CTGCTACGTCTgg                                                       >  1:110115/1‑251 (MQ=255)                                                                                                                                                                                                     aaaaaTAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTCCATCCCCAAAGCGCCAACTTAAGGG‑GTGTGGGATTACCCCTGGTATGGTTTTACATGCCCATTGATTACTCTCCCGATAAATTGGCAGCAATTCTGGCCCCTATTGGTAAAACCGCAGAAATGGATAATTCGGCACGTAAATGC‑GGGGGTCTAAACCCCCCCCCCGAAAATAGTGATGCTGC‑AACACTGCtccggccggg                                                      >  1:310751/1‑242 (MQ=255)                                                                                                                                                                                                                gCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAA‑TGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGC‑AACTCTGCTACGTCTGGGGCTGGCTTACg                                           <  1:160687/251‑1 (MQ=255)                                                                                                                                                                                                                     gAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAA‑TGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGC‑AACTCTGCTACGTCTGGGGCTGGCTTACGGccc                                       <  2:310751/250‑1 (MQ=255)                                                                                                                                                                                                                           atGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAA‑TGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGC‑AACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCgggggg                                <  1:98719/251‑1 (MQ=255)                                                                                                                                                                                                                                                     agcccTCCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTATGCCTGATTTGATTTAACGTGCCGATGAATTACTCGCACGAAAACTGGTCAGCAATGCTGGCCCATATTTGTAAGCCCGAAGAACTGGTTACTTCGGCACGTAA‑TGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGC‑AACGCTGCTACGTCTTGGGCTGGCTTGCGGACCCGGGGGTATTTCATGACGGGAAGTCACTGCATg      <  2:383936/247‑1 (MQ=11)                                                                                                                                                                                                                                                         cTCCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCCGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAA‑TGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGC‑AACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTACGTGAAGTCACTGCATGGGCt  >  2:254399/1‑251 (MQ=11)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                             GCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGG‑TTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAA‑TGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGC‑AACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTACGTGAAGTCACTGCATGGGCT  >  NC_000913/15138‑15636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.