Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,468,298	IS3 (+) +3 bp	coding (85‑87/921 nt)	yfdH →	bactoprenol glucosyl transferase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2170171 =	NA (NA)	27 (0.630)	25/492	0.5	100%	noncoding (1/1258 nt)	IS3	repeat region
	?	NC_000913	= 2468300	0 (0.000)					coding (87/921 nt)	yfdH	bactoprenol glucosyl transferase
*	?	NC_000913	= 2171428	NA (NA)	31 (0.730)	28/490	0.4	100%	noncoding (1258/1258 nt)	IS3	repeat region
	?	NC_000913	2468298 =	0 (0.000)					coding (85/921 nt)	yfdH	bactoprenol glucosyl transferase

CATCAGCACCGACAAACATTAAATCGGCATTAATATCCTGTAATGAACGCTCGGCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCC‑CGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATCAGCAGACGTCTTCTGA  >  NC_000913/2169929‑2170417                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                        cctCAGCACCGACAAACATTAAATCGGCATTAATCTCATGTAATGAACGCTCGGCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGGCCGTGCCACCACAGACGACCAGAGTAATGTCTCTATATTGGGAAAGGGCAAACGCTGACGGGAGCCTGTTGGTAATCACCGTGATATTTTTGGCGGTCCTTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCCGACGAGCATAACGGGCTGATCTTAc                                                                                                                                                                                                                                                 >  1:289947/3‑251 (MQ=255) cATCAACACCGACAAACATTAAATCGGCATTAATATACTGTAATGAACGCTCGGCAATGGAACCATGCATCGAGCGCGTTTTATGTCGGACCGAGCCACCACAGAGGACCAGAGCAATGTCTTTATATTCGGACAGGTCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGAGGTCATTCATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAAAGGTCTGaatttac                                                                                                                                                                                                                                                 >  1:289943/1‑245 (MQ=255)        aCCGACAAACATTAACTCGGCATTAATATCCTGTAATGACCGCTCGGCAATGGAAACATGCATCGAGCGCGTTTTATGGAGGACCGAGCCACCACAGACGACACGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGCTATTTTTGGCGGTCATTAATCCTTCGGCGCTAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCa                                                                                                                                                                                                                                           >  1:154232/1‑250 (MQ=255)                  aTTAAATCGGCATTAATATCCTGTAATGAACGCTCGGCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGATCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGacac                                                                                                                                                                                                                                <  2:128685/251‑1 (MQ=255)                                                                         aGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTAt                                                                                                                                                                         <  2:120479/251‑1 (MQ=255)                                                                            gcgTTTTATGGCGGACCGTGCCACAACCGACGACCCGAGTCATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCa                                                                                                                                                                       >  2:418510/1‑250 (MQ=255)                                                                                  tATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGGAAACTCTCAGTCCGCGGTGACTCACATGCCCAAACCAGCATCAACCAgc                                                                                                                                                                 >  2:120723/1‑249 (MQ=39)                                                                                       cGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTGCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTGTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTTATCGTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTaaaaaa                                                                                                                                                           <  2:73676/251‑1 (MQ=255)                                                                                        ggACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAAc                                                                                                                                                          >  1:81940/1‑251 (MQ=255)                                                                                                                                    gAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGCCAAAAACAGTATCAACCAGTAAAAAACCC‑CGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGa                                                                                                               >  2:248293/1‑249 (MQ=255)                                                                                                                                       aGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCC‑CGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCtt                                                                                                          <  2:81940/251‑1 (MQ=255)                                                                                                                                                           aCTGTTGGCAATCCCCGTGATATTTTTGGCGGACATTCATCCTTCGGCGATAAGCATTGTCGTACTTCAGCTGTCGAGAATAACGGTCTGATCTTAACCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATAACAAAAACAGTATCAACCAGTAAAAAACCC‑CGTAAACCGCATTCGCCTGACTTTCGCAGACACGCCCTGAAGCTTGCTGAACGCATCGGTGTTAc                                                                                      >  2:414729/1‑251 (MQ=18)                                                                                                                                                                tGGTACTCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTAGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCC‑CGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCg                                                                                 >  2:396450/1‑251 (MQ=255)                                                                                                                                                                   tAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCC‑CGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGc                                                                                >  1:107194/1‑249 (MQ=255)                                                                                                                                                                     aTCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCC‑CGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGcc                                                                            <  1:28901/251‑1 (MQ=255)                                                                                                                                                                                       gCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCC‑CGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTAt                                                          >  1:358560/1‑251 (MQ=255)                                                                                                                                                                                            cATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCC‑CGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAAt                                                      <  1:176145/250‑1 (MQ=255)                                                                                                                                                                                                     ttCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGAGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCC‑CGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAActct                                             >  1:237969/1‑250 (MQ=255)                                                                                                                                                                                                                      tcgaacacccGCTCTCGAGAAAAACGAACTCATCATAACCAGCAATAGTGCACCAGCGGCTACGTCCATCAACTCTCACTCAGAGCCGACAAACAAGAAAAAAACAGTATCAACCAG‑AAAAAACCCACGCAAACAGCCTTCGCCTGACTTTCGCAATGAAGAACTGAAGCTTGCTGAACGCATCGGTGTTACTGCAGCAGCCCGTGCACTCAGCCTGCATGAATCACAACTCTACAACTGGCGCAGTaacc                           >  2:174151/9‑249 (MQ=2)                                                                                                                                                                                                                                 cTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCC‑CGTAAACAGCATTCGCCTTAATTTCGCa                                                                                                                           <  2:382436/144‑1 (MQ=255)                                                                                                                                                                                                                                 cTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCC‑CGTAAACAGCATTCGCCTGAATTTCGCa                                                                                                                           >  1:382436/1‑144 (MQ=255)                                                                                                                                                                                                                                                gTCTGATCTTACCCCGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATAAACCAGTAAAAAACCC‑CGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATCAGCAGACGTCTTCTGa  >  1:373687/1‑250 (MQ=11)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                        CATCAGCACCGACAAACATTAAATCGGCATTAATATCCTGTAATGAACGCTCGGCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCC‑CGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATCAGCAGACGTCTTCTGA  >  NC_000913/2169929‑2170417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.