Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,361,732	IS5 (–) +4 bp	coding (557‑560/765 nt)	gltF →	periplasmic protein GltF

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3361732 =	0 (0.000)	23 (0.680)	20/486	0.6	100%	coding (557/765 nt)	gltF	periplasmic protein GltF
	?	NC_000913	= 3366750	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region
*	?	NC_000913	= 3361735	0 (0.000)	37 (1.090)	35/486	0.1	100%	coding (560/765 nt)	gltF	periplasmic protein GltF
	?	NC_000913	3365556 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region

GGTTTCCAGCGGATAAGGTCGCCGGCCATTACCAGCCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTACTTCCCCCAATAATTGGCTGGTGTTTATGCAAAATGGTCAAGAAGTGGTAATTGATAGCGGTAAAAGCGTTAGCCTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGAT  >  NC_000913/3366506‑3366991                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  ggTTTCCAGCGGATAAGGTCGCCGGCCATTACCAGCCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTa                                                                                                                                                                                                                                             <  1:2201/251‑1 (MQ=11)    ttCCAGCGGATAAGGTCGCCGGCCATTACCAGCCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTACt                                                                                                                                                                                                                                           <  1:44367/250‑1 (MQ=31)       cAGCGGATAAGGTCGCCGGCCATTACCAGCCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTACTTccc                                                                                                                                                                                                                                       <  2:120531/251‑1 (MQ=32)       cAGCGGATAAGGTCGCCGGCCATTACCAGCCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTACTTccc                                                                                                                                                                                                                                       >  1:37718/1‑251 (MQ=32)        aGCGGATAAGGTCGCCGGCCATTACCAGCCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGGCAGAATCTGCGCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTACTTcccc                                                                                                                                                                                                                                      <  1:190499/251‑1 (MQ=18)               aaGGGCGCCTGCCATTACCAGCCTTGGGGTAAAACGGCTCTATGACTTCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAAGTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAGTCCTTGTCGATGTCTCCAGATTACAAACATGATCTCATATCAGGGACTTGTTCTCACCTGTCTTAGTACTTCCCCCaataat                                                                                                                                                                                                                               <  2:342860/251‑1 (MQ=11)                aGGTCGCCGGCCATTACCAGCCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTACTTCCCCCAATAAtt                                                                                                                                                                                                                              <  1:91312/251‑1 (MQ=37)                              ttccAGCCTTGGGGTAAAACGGCTCGATGACGTCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTTTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGTCAAACATGATCTCATATCAGGGATTTGTTTGCAGCTTCCTTAGTACTTCCCTCAAGAATCGGCTGGTGTTTAt                                                                                                                                                                                                                 <  2:44084/248‑1 (MQ=11)                                    ccTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTACTTCCCCCAATAATTGGCTGGTGTTTATGCAAAAt                                                                                                                                                                                                          >  1:61243/1‑251 (MQ=255)                                                           aCTTCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACGTGTTCGCACCTTCCTTAGTACTTCCCCCAATAATTGGCTGGTGTTTATGCAAAATGGTCAAGAAGTGGTAATTGATAg                                                                                                                                                                                   <  1:313223/251‑1 (MQ=255)                                                                              aTGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCTCCTTCCTTAGTACTTCCCCCAATAATTGGCTGGTGTTTATGCAAAATGGTCAAGAAGTGGTAATTGATAGCGGTAAAAGCGTTAGCCTc                                                                                                                                                                <  1:15417/251‑1 (MQ=255)                                                                                ggCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGAATCATGATGAACCCTGTTCTATGGATCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTACTTCCCCCAATAATTGGCTGGGGTTTATGCAAAATGGTCAAGAAGTGGTAATTGATAGCGGTAAAAGCGTTAGCCTcac                                                                                                                                                              >  1:172732/1‑251 (MQ=255)                                                                                 gCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTACTTCCCCCAATAATTGGCTGGTGTTTATGCAAAATGGTCAAGAAGTGGTAATTGATAGCGTTAAAAGCGTTAGCCTcaca                                                                                                                                                             <  2:37718/251‑1 (MQ=255)                                                                                     aaTCTGCTCCATGCGTGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTACTTCACCCAATAATTGGCTGGTGTTTATGCAAAATGGTCAAGAAGTGGTAATTGATAGCGGTAAAAGCGTTAGCCTCACACTg                                                                                                                                                          >  1:130147/1‑250 (MQ=255)                                                                                     aaTCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTTACGGCGCTTACTGCTGAATTCACTGACGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTACTTCCCCCAATAATTGGCTGGTGTTTATGCAAAATGGTCAAGAAGTGGTAATTGATAGCGGTAAAAGCGTTAGCCTCTCACTGa                                                                                                                                                         <  1:335250/251‑1 (MQ=255)                                                                                           cTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTACTTCCCCCAATAATTGGCTGGTGTTTATGCAAAATGGTCAAGAAGTGGTAATTGATAGCGGTAAAAGCGTTAGCCTCACACTGACAATgg                                                                                                                                                   >  2:86363/1‑251 (MQ=255)                                                                                                       cAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTACTTCCCCCAATAATTGGCTGGTGTTTATGCAAAATGGTCAAGAAGTGGTAATTGATAGCGGTAAAAGCGTTAGCCTCACACTGACAATGGCTCCAGCTTTTa                                                                                                                                       <  1:472/251‑1 (MQ=255)                                                                                                                               ccGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTACTTCCCCCAATAATTGGCTGGTGTTTATGCAAAATGGTCAAGAAGTGGTAATTGATAGCGGTAAAAGCGTTAGCCGCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACa                                                                                                               <  2:228243/250‑1 (MQ=255)                                                                                                                                cGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTGCTATGGCTCCAGATGACAAACATTATCGCATATCAGGGACTTGTTCGCATCTTCCTTAGTACTTCCCCCAATAATTGGCTGGTGTTTATGCAAAATGGGCAAGAAGTGGTAATTGATAGCGGTAAAAGCGTTAGCCTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACAt                                                                                                              <  1:340664/251‑1 (MQ=255)                                                                                                                                 ggCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTACTTCCCCCAATAATTGGCTGGTGTTTATGCAAAATGGTCAAGAAGTGGTAATTGATAGCGGTAAAAGCGTTAGCCTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATg                                                                                                             >  1:317874/1‑251 (MQ=255)                                                                                                                                               aaTTCACTGTCGGCGCAGGTAAGTTGATGACTCATGATGAACACTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCATTAGTACTTCCCCCAATAATTGGCTGGTGTTTATGCAAAATGGTCTAGAAGTGGTAATTGATAGCGGTAAAAGCGTTAGCCTCACACTGACAATg                                                                                                                                                    >  2:213566/1‑198 (MQ=255)                                                                                                                                               aaTTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGTCAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTACTTCCCCCAATAATTGGCTGGTGTTTATGCAAAATGGACAATAAGTGGTAATTGATAGCGGTAAAAGCGTTAGCCTCACACTGACAATg                                                                                                                                                    <  1:213566/198‑1 (MQ=255)                                                                                                                                               aaTTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTACTGCCCCCAATAATTGGCGGGTGTTTATGCAAAATGGTCAAGAAGTGGTAATTGATAGCGGTAAAAGCGTTAGCCTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAgg                                                                                               <  2:130147/251‑1 (MQ=255)                                                                                                                                                                    aGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTACTTCCCCCAATAATTGGCTGGTGTTTATGCAAAATGGTCAAGAAGTGGTAATTGATAGCGGTAAAAGCGTTAGCCTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCtggtgg                                                                           >  1:241071/1‑250 (MQ=255)                                                                                                                                                                     gTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTACTTCCCCCAATAATTGGCTGGTGTTTATGCAAAATGGTCAAGAAGTGGTAATTGATAGCGGTAAAAGCGTTAGCCTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCtggtgg                                                                           >  1:89847/1‑249 (MQ=255)                                                                                                                                                                                 tgatgaACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTACTTCCCCCAATAATTGGCTGGTGTTTATGCAAAATGGTCAAGAAGTGGTAATTGATAGCGGTAAAAGCGTTAGCCTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCATGGTGGAGGCACAATGAAc                                                              >  2:165772/1‑250 (MQ=255)                                                                                                                                                                                     gaACCCTGTTCTATGGCGCCAGATGACAAACATGATCTCATATCAGGGACTTGGTCGCAACTTCCTTAGTACTTCCCCCAATAATTGGCTGGTGTTTATGCAAA‑TGGTCAAGAAGTGGTAATTGATAGCGGTAAAAGCGTTAGCCTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTa                                                        <  2:65264/251‑1 (MQ=255)                                                                                                                                                                                        cccTGTTCTATTGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCGTCCTTAGTACTTCCCCCAATAATTGGCTGGTGTTTAAGCAAAATGGTCAAGAAGTGGTAATTGATAGCGGTAAAAGCGTTAGCCTCACACTGACAATGGATCCATCTGTTAAGGATGAAGGGGAACTAACCGACATTACAGATATTACAGGCAATTTGACGGTCCTGGTGGAGGCAAAATGAACAATGTaaa                                                      <  1:33837/251‑1 (MQ=39)                                                                                                                                                                                                 aTGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTACTTCCCCCAATAATTGGCTGGTGTTTATGCAAAATGGTCAAGAAGTGGTAATTGATAGCGGTAAAAGCGTTAGCCTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGa                                              <  1:165772/250‑1 (MQ=255)                                                                                                                                                                                                   ggCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTACTTCCCCCAATAATTGGCTGGTGTTTATGCAAAATGGTCAAGAAGTGGTAATTGATAGCGGTAAAAGCGTTAGCCTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGTAGGCAAAATGAACAATGTAAAATTACTGATTg                                           <  1:304750/251‑1 (MQ=255)                                                                                                                                                                                                     cTCCAGATGACAACCATGATCTCATATCAGGGACTTGTTAGCACCTTCCTTAGTACTTCCCCCAATAATTGGCTGGTGTTTATGCAAAATGGTCAAGCAGTGGTAATTGATAGCGGTAAAAGCGTTAGCCTCACACTGACAATGGCTCCAGCTTTTACGGATGAAGGGTAACTAACCGACATGACAGATATTACAGACAATCTGACGGTCCTGGTGGAGGCAACATGAACAATGAAAAATTACTGATTGcc                                         >  2:359920/1‑251 (MQ=255)                                                                                                                                                                                                                                    gACTTGTTCGCACCTTCCTTAGTACTTCCCCCAATAATTGGCTGGTGTTTATGCAAAATGGTCAAGAAGTGGTAATTGATAGCGGTAAAAGCGTTAGCCTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCa            >  2:114809/1‑249 (MQ=255)                                                                                                                                                                                                                                              cACCTTCCTTAGTACTTCCCCCAATAATTGGCTGGTGTTTATGCAAAATGGTCAAGAAGTGGTAATTGATAGCGGTAAAAGCGTTAGCCTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGAt  >  1:251481/1‑249 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  GGTTTCCAGCGGATAAGGTCGCCGGCCATTACCAGCCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGTACTTCCCCCAATAATTGGCTGGTGTTTATGCAAAATGGTCAAGAAGTGGTAATTGATAGCGGTAAAAGCGTTAGCCTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGAT  >  NC_000913/3366506‑3366991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.