Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,228,095	IS2 (+) +5 bp	coding (17‑21/660 nt)	ycgM →	putative isomerase/hydrolase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1228095 =	0 (0.000)	27 (0.800)	24/482	0.4	100%	coding (17/660 nt)	ycgM	putative isomerase/hydrolase
	?	NC_000913	1467910 =	NA (NA)					noncoding (1331/1331 nt)	IS2	repeat region
*	?	NC_000913	= 1228099	0 (0.000)	23 (0.680)	22/486	0.5	100%	coding (21/660 nt)	ycgM	putative isomerase/hydrolase
	?	NC_000913	= 1469240	NA (NA)					noncoding (1/1331 nt)	IS2	repeat region

TTACGCCAGAGAAATAACTGGCTGGCTGCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCA‑GAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATACT  >  NC_000913/1468998‑1469489                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                           ttACGCCAGAGAAATAACTGGCTGGCTGCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAAcccc                                                                                                                                                                                                                                                      >  1:179862/1‑249 (MQ=31)               tAACTGGCTGGCTGCTACACCATGTTGCCGGGCAACGAGAGAGACCGTCATCCCCGGTTCAAAGCACTGCTGAACAATTGAGATCTTTTCCAGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAACAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCA‑GAACTTACTTATGCt                                                                                                                                                                                                                                     >  1:356739/1‑251 (MQ=255)                    ggctggctGCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCA‑GAACTTACTTATGCTGAtt                                                                                                                                                                                                                                 <  1:214639/250‑1 (MQ=40)                         gctgctACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCA‑GAACTTACTTATGCTGATTCCGGTg                                                                                                                                                                                                                           <  2:179862/251‑1 (MQ=255)                              tACACCATGTTGCCGGGCCACGAGGGAGACCGTAATCCCCGGCTCAAAGCTCTGCTGAACAATTGAGATCTTTTCCTGTGCGCAACGGCGTCTCCGTTACTCCGGCCCTAATACGTCAATCATCTGTTCTCCAATGACTCGTCTAAACACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAA‑CCACACGACATTACTTATGCTGATCCCGGTGCGaa                                                                                                                                                                                                                       >  2:148946/1‑250 (MQ=11)                                      gTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCA‑GAACTTACTTATGCTGATTCCGGTGCGAAAATTGTta                                                                                                                                                                                                               <  1:170731/250‑1 (MQ=255)                                           cGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCA‑GAACTTACTTATGCTGATTCCGGTGCGAAAATTGTtaataa                                                                                                                                                                                                           >  1:334001/1‑249 (MQ=255)                                                                          cAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCA‑GAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAAc                                                                                                                                                                           <  2:262359/250‑1 (MQ=255)                                                                                                     tttCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCA‑GAACTGACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGAGTCAATGGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCg                                                                                                                                                  <  1:198470/248‑1 (MQ=255)                                                                                                           gtgtgGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCA‑GAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGttttt                                                                                                                                         <  1:41153/251‑1 (MQ=255)                                                                                                                                            aGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCA‑GAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGaaaa                                                                                                         >  1:151946/1‑250 (MQ=255)                                                                                                                                                    atcatcTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCA‑GAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGt                                                                                                <  2:334001/251‑1 (MQ=255)                                                                                                                                                                ccactGACTAGTCTAACAACTAGTATTAAGACTATCACTTATTTAAGCGATATTGGTTGCCTGGAGATTCAGGGTGCCAGTCTAAACCCCA‑GAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATg                                                                                    >  2:27019/5‑251 (MQ=255)                                                                                                                                                                              aaaaaCTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCA‑GAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAGCATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAAc                                                                        <  2:159646/249‑1 (MQ=255)                                                                                                                                                                              aaaaaCTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCA‑GAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGTAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAAc                                                                        <  1:344271/249‑1 (MQ=255)                                                                                                                                                                                                 aTCACTTATTTAAGAGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCA‑GAACTTACTTATGCTGATTCCGGTGCGAAAAGTGTTAATAAAGGTAGTTGTAGATTCAATTGGACAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTGTTGTGAATGAGACGAACATGGACGGCAGAGTAAAAAGCCGCTGGTTTTGAGCTATGGAAGAACATAACAGGCTTCAGTGAAATAGCGaa                                                   <  2:163088/251‑1 (MQ=21)                                                                                                                                                                                                                          gTCTGGAGATTCAGGGGGCCAGTCTAAACCCCA‑GAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCAGGAACg                          >  1:112437/1‑251 (MQ=255)                                                                                                                                                                                                                          gTCTGGAGAGTCAGGGGGCCAGTCTAAACCCCA‑GAACTTACGTATGCTGAGTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTGGCGGAGGTTTTGTGAATGAGACGAACATTTACAGCAGAGGAAAAATCCTCTGTGTGTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGTGTTCAAAACCCGGAAc                           <  2:31408/250‑1 (MQ=18)                                                                                                                                                                                                                                                   aaaCCCCA‑GAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGAACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTGTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATAGCCTGTGTGCAAAACCCGGAACGATCTTCACTATGTTAAGGGATACt  <  2:250143/250‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                           TTACGCCAGAGAAATAACTGGCTGGCTGCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCA‑GAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATACT  >  NC_000913/1468998‑1469489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.