Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,317,225	IS2 (–) +5 bp	coding (2648‑2652/2850 nt)	rcsC ←	histidine kinase RcsC

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1651548	NA (NA)	42 (1.230)	40/486	0.1	97.6%	noncoding (706/706 nt)	IS2	repeat region
	?	NC_000913	= 2317229	1 (0.030)					coding (2648/2850 nt)	rcsC	histidine kinase RcsC
*	?	NC_000913	2317225 =	1 (0.030)	40 (1.180)	38/486	0.1	97.5%	coding (2652/2850 nt)	rcsC	histidine kinase RcsC
	?	NC_000913	= 2997691	NA (NA)					noncoding (1/1331 nt)	IS2	repeat region

GCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATC  >  NC_000913/2316975‑2317471                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                       gCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACGCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCgg                                                                                                                                                                                                                                                        <  1:139150/251‑1 (MQ=255)        ttacgcaagagaaataactggctggctgctacaccatggtgcagggcaacgagggagaccgtcatccccggttcaaagctctgctgaacaattgcgatcttttcctgtgtggtacgccgtctgcgtttctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagagatattggttgtctggagattcagagggccagactaGTAAccat                                                                                                                                                                                                                                                 <  2:63176‑M1/8‑1 (MQ=255)        gCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACtgg                                                                                                                                                                                                                                                 <  1:98440‑M2/251‑4 (MQ=255)              cagagaaataactggctggctgctacaccatgttgccgggcaacgagggagaccgtcatccccggttcaaagctctgctgaacaattgcgatcttttcctgtgtggtacgccgtctgcgtttctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGTAACCATCCatat                                                                                                                                                                                                                                           >  1:253051‑M1/238‑251 (MQ=255)                 agaaataactggctggctgctacaccatgttacagggcaacgagggagaccgacatccccggttcaaagctctgctgaacaattgcgatcttttcctgtgtggtacgccgtctgcgtttctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGTAACCATCCATATTTg                                                                                                                                                                                                                                        <  1:209926‑M1/17‑1 (MQ=255)                          ggATGCGGCGTAAACGCCTTATCCGTCCTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAGCGTAAGCGTCTGTTTTATCACTTCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGCCGAATGCGTTGCGTCACGCGGTAACtggatttgcccctatatttcc                                                                                                                                                                                                                               <  2:358299‑M2/251‑22 (MQ=255)                              gcggccTAAACGACTTCTCCGTCCTACGAATCCCGCGATTTACTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGCCAGGCAGCTGTCCATACCGGCCTCCCGACACCGCTGCTTCTCTTCCGCCAACGCATTAGCAGTTACTCCGATTCCCGGCAACGTACGTCCCAACTGACGAATGCGTTGCGTCCCGCGGTAACtggatttgcccctatatttccagac                                                                                                                                                                                                                           <  2:205067‑M2/251‑26 (MQ=255)                                  cgTAACCGCCTTATCCGTCCTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGCTTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACtggatatgcccctatatttccagccatct                                                                                                                                                                                                                       <  2:260565‑M2/251‑30 (MQ=255)                                        cGCCTTATCCGTCCTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACtggatttgcccctatatttccagacatctgttatc                                                                                                                                                                                                                 >  1:352567‑M2/1‑216 (MQ=255)                                        accatgttgccgggcaacgagggagaccgtcatccccggttcaaagctctgctgaacaattgcgatcttttcctgtgtggtacgccgtctgcgtttctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcactgatttaagtgatattggttgtctggagatgcagggggccagtctaGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACg                                                                                                                                                                                                                 >  1:16335‑M1/212‑251 (MQ=255)                                                 ccgggcaacgagggagaccgtcatccccggtccaccgcactgctgaacacttgcgatcttttcctgtgtggtacgccgtctgcgtttctacggtcctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGTAACCATCCATCTTTGGCATGTTGACGTCGCTAAGcacgatataaat                                                                                                                                                                                                         <  1:54714‑M1/48‑5 (MQ=255)                                                  cgggcaacgagggagaccgtcatccccggttcaaagctctgctgaacaattgcgatcttttcctgtgtggtacgccgtctgcgtttctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAatat                                                                                                                                                                                                       >  1:343306‑M1/202‑251 (MQ=255)                                                  cgggcaacgagggagaccgtcatccccggttcaaagctctgctgaacaattgcgatcttttcctgtgtggtacgccgtctgcgtttctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAatat                                                                                                                                                                                                       >  2:112389‑M1/202‑251 (MQ=255)                                                    ccTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGGAACtggatttgcccctatatttccagacatctgttatcacttaacccatt                                                                                                                                                                                                     <  2:260061‑M2/251‑48 (MQ=255)                                                         gAATCCCGCGCTATCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCCGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCACTTACCGGCAACGTCAGTCCCAATAGACGAATGCGTTGCGTCACGCGGCAACtggatttgcccctatatttccagacctctgttatcacttaacccattacaa                                                                                                                                                                                                 <  2:91062‑M2/250‑52 (MQ=255)                                                          gaggtagaccgtcatccccggttcaaagctctgctgaacaattgcgatcttttcctgtgtggtacgccgtctgcgtttctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTg                                                                                                                                                                                               <  2:200264‑M1/58‑1 (MQ=255)                                                           agggagaccgtcatccccggttcaaagctctgctgaacaattgcgatcttttcctgtgtggtacgccgtctgcgtttctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGc                                                                                                                                                                                              <  1:116032‑M1/59‑1 (MQ=255)                                                           agcccgcGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcc                                                                                                                                                                                              >  2:52859‑M2/3‑197 (MQ=255)                                                            gggagaccgtcatccccggttcaaagctctgctgaacaattgcgatcttttcctgtgtggtacgccgtctgcgtttctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCt                                                                                                                                                                                             <  1:283695‑M1/60‑1 (MQ=255)                                                                    gtcatccccggttcaaagctctgctgaacaattgcgatcttttcctgtgtggtacgccgtctgcgtttctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACAt                                                                                                                                                                                     <  1:57037‑M1/68‑1 (MQ=255)                                                                    gtcatccccggttcaaagctctgctgaacaattgcgatcttttcctgtgtggtacgccgtctgcgtttctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACAt                                                                                                                                                                                     <  2:300422‑M1/68‑1 (MQ=255)                                                                      catccccggttcaaagctctgctgaacaattgcgatcttttcctgtgtggtccgccgtctgcgtttctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACAtt                                                                                                                                                                                    <  1:129730‑M1/69‑1 (MQ=255)                                                                          gACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagat                                                                                                                                                                                 >  1:67467‑M2/1‑182 (MQ=255)                                                                          cccggttcaaagcacggctgaacaattgcgatcttttcctgcgtggtacgccatctgcgtttctccggccctacggcatcaatcatctgatctcccatggctagtctaaaaactcgtattaagactatcacttctttaagtgatattggttgtctggagattcagggagccagtctaGTAACCATCCATATTTGGCATGTTGACGTCGTTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAgcg                                                                                                                                                                               <  2:174703‑M1/74‑1 (MQ=255)                                                                           ccggttcaaagctctgctgaacaattgcgatcttttcctgtgtggtacgccgtctgcgtttctccggccctaagacatcaatcagctgttctccaatgactagtctaaaaactagtattaagactatctcttatttaagtgatattgggtgtctggagattcagggggccaggctaGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAgcgc                                                                                                                                                                              >  2:101423‑M1/177‑251 (MQ=255)                                                                           aCCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACtggatttgcccctatatgtccagacatctgttatcacttaacccattacaagcccgctgccgcagatatt                                                                                                                                                                              >  2:357873‑M2/1‑181 (MQ=255)                                                                             ccTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcc                                                                                                                                                                            >  2:241742‑M2/1‑179 (MQ=255)                                                                                ctcGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattccc                                                                                                                                                                           >  1:273493‑M2/1‑176 (MQ=255)                                                                                 caaagctctgctgaacaattgcgatcttttcctgtgtggtacgccgtctgcgtttctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGAc                                                                                                                                                                        <  1:328268‑M1/81‑1 (MQ=255)                                                                                      atatAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGCCACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCACCGTTAGTCCTAACTGACGAATGAGTTGCGTCCAGCGGTAACtggatttgcacctatatttccagacatcttttattacttaacaccatacaagcccgctcccgcaaatattcccgtggcgtg                                                                                                                                                                   <  2:79235‑M2/251‑82 (MQ=255)                                                                                        atgctgaacaattgcgatcttttcctgtgtggtacggcgtctgcgtttctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatagtggttgtctggagattcagggggccagtctaGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGCcat                                                                                                                                                                   >  1:239638‑M1/164‑249 (MQ=255)                                                                                         tgctgaacaattgcgatcttttcctgtgtggtacgccgtctgcgtttctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGTAACCATCCATATTTg                                                                                                                                                                                                                                        <  1:353895‑M1/17‑1 (MQ=255)                                                                                         tgcggaacaattgcgatcttttcctgtgtggtacgccgtctgcgtttctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGTAACCATCCATATTTg                                                                                                                                                                                                                                        >  2:353895‑M1/163‑179 (MQ=255)                                                                                             gaacaattgcgatcttttcctgtgtggtccgccgtctgcgtttctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGc                                                                                                                                                                                              <  2:358220‑M1/59‑1 (MQ=255)                                                                                             gaacaattgcgatcttttcctgtgtggtacgccgtctgcgtttctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGTAACCATCCATATTTGGCATTTTGACGTCGCTAAGCACGATATCAATATGATTCTTGc                                                                                                                                                                                              >  1:358220‑M1/159‑217 (MQ=255)                                                                                              tCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacc                                                                                                                                                           <  1:50839‑M2/251‑90 (MQ=255)                                                                                                  attgcgatcttttcctgtgtggtacgccgtctgcgtttctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCGCGGttt                                                                                                                                                       >  2:10972‑M1/154‑251 (MQ=255)                                                                                                   gTCTGTTTTATCACATCCCGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACtggatttgcccctatctttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcg                                                                                                                                                      <  2:31388‑M2/251‑95 (MQ=255)                                                                                                      tGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCAGATTCCCGGCAACGTAAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACtggatttgcccctatatttcccgacatctgttatcacttaacccattacacgcccgctgccgcagatattcccgtggcgagcgctaacccagcgcac                                                                                                                                                   <  2:71‑M2/251‑98 (MQ=255)                                                                                                       gTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcac                                                                                                                                                   >  1:339888‑M2/1‑153 (MQ=255)                                                                                                              cacaTCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcgg                                                                                                                                            <  1:307021‑M2/250‑105 (MQ=255)                                                                                                                        gTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAACCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgt                                                                                                                                 <  1:51725‑M2/251‑116 (MQ=255)                                                                                                                         acgccgtctgcgtttctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGACATCATTCGCGGTTTTACATTGATAGCCCAACGATccc                                                                                                                                <  2:27769‑M1/121‑1 (MQ=255)                                                                                                                          tACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACtggatttgcccctatatttccagacatctgttatcacttaacccattaca                                                                                                                                                                                                  <  1:150393‑M2/184‑51 (MQ=255)                                                                                                                          tACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACtggatttgcccctatatttccagacatctgttatcacttaacccattaca                                                                                                                                                                                                  >  2:150393‑M2/1‑134 (MQ=255)                                                                                                                            ccgtctgcgtttctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAAc                                                                                                                             >  1:194797‑M1/128‑251 (MQ=255)                                                                                                                                   cgtttctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCACAACTGATCTg                                                                                                                      <  1:32495‑M1/131‑1 (MQ=255)                                                                                                                                      cAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACTAATGCGTTGCGTCAAGCGGTAACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattccctttgcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaac                                                                                                                   >  1:310621‑M2/1‑122 (MQ=255)                                                                                                                                           aGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACtggatttgccccgatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacgcctc                                                                                                              >  2:43704‑M2/1‑117 (MQ=255)                                                                                                                                                  cATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacgcctctgcaagg                                                                                                       >  2:270197‑M2/1‑110 (MQ=255)                                                                                                                                                   aTACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacgcctctgcaaggt                                                                                                      <  1:363958‑M2/251‑143 (MQ=255)                                                                                                                                                    tACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacgcctctgcaaggtt                                                                                                     <  2:73931‑M2/251‑144 (MQ=255)                                                                                                                                                       atcaatcatctgttctcgaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtcttgagattcagggggccagtctaGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATc                                                                                                  >  2:37245‑M1/101‑251 (MQ=255)                                                                                                                                                           cTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattc                                                                                                                                                                             >  2:193625‑M2/1‑101 (MQ=255)                                                                                                                                                           cTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattc                                                                                                                                                                             <  1:193625‑M2/172‑72 (MQ=255)                                                                                                                                                           atcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGgat                                                                                              <  1:265801‑M1/155‑1 (MQ=255)                                                                                                                                                               aGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacgcctctgcaaggttctttgctgccg                                                                                          <  2:272001‑M2/251‑155 (MQ=255)                                                                                                                                                                  gttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGatcatc                                                                                       <  1:14177‑M1/162‑1 (MQ=255)                                                                                                                                                                             actagtctaaaaactagtattaagcctatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATCTGATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGa                                                                             <  1:161881‑M1/172‑1 (MQ=255)                                                                                                                                                                                    taaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATcatcat                                                                     <  2:120219‑M1/180‑1 (MQ=255)                                                                                                                                                                                     aaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAAtcatca                                                                      >  2:37707‑M1/71‑249 (MQ=255)                                                                                                                                                                                       aaCGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCTAACTGACGAATGCGTTGCGTCAAGCGGCAACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcc                                                                                                                                                                            <  1:349455‑M2/145‑73 (MQ=255)                                                                                                                                                                                       aaCGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACtgtatttgcccctatatttccagacatctgtgatcacttaacccattacaagcccgctgccgcagatattcc                                                                                                                                                                            >  2:349455‑M2/1‑73 (MQ=255)                                                                                                                                                                                       aaCGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacgcctctgcaaggttctttgctgccgttaacccgtctggtttgggcatg                                                                   <  1:89782‑M2/250‑178 (MQ=255)                                                                                                                                                                                            agtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTc                                                               >  2:353688‑M1/64‑249 (MQ=255)                                                                                                                                                                                               aGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacgcctctgcaaggttctttgctgccgttaacccgtctggtttgggcatgatactgat                                                           >  1:272001‑M2/1‑65 (MQ=255)                                                                                                                                                                                                      aCTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTCATCTGCCAGCAAACGCCGGTTAATCGGATGACCATCCACGACCAGAATCATCATATCGTCATTATCGCt                                                      >  2:77343/1‑248 (MQ=255)                                                                                                                                                                                                         ccGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGTGAACtggatttgcccctatatctccatacatctgttatcacttaacccattacaagcccgctgccgcagagattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacgcctctgcaaggttctttgctgccgttaacccgtctggtttgggcatgatgctgatgtagtcacg                                                  >  2:62649‑M2/1‑55 (MQ=255)                                                                                                                                                                                                           acttatttaagtgatattggttgtctggagattcagggggccagtctaGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCtt                                              <  2:353333‑M1/203‑1 (MQ=255)                                                                                                                                                                                                                ccGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacgcctctgcaaggttctttgctgccgttaacccgtctggtttgggcatgatactgatgtagtcacgctttatcgt                                         <  1:132676‑M2/251‑204 (MQ=255)                                                                                                                                                                                                                     aaCGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacgcctctgcaaggttctttgctgccgttaacccgtctggtttgggcctgatactgatgtagtcacgctttatcgttttcg                                    <  2:327302‑M2/251‑209 (MQ=255)                                                                                                                                                                                                                     aaCGTCAGGCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacgcctctgcaaggttctttgctgccgttaacccgtctggtttgggcatgatactgatgtagtcacgctttatcgtttt                                      >  1:327302‑M2/1‑43 (MQ=255)                                                                                                                                                                                                                               ccAACTGACGAATGCGTTGCGTCAAGCGGTAACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacgccgcggcaaggttctttgctgccgttaacccgtctggtgttggcctgatactgatgtagtctcgctttctcgttttcgcgaagctctc                          >  2:41967‑M2/1‑33 (MQ=255)                                                                                                                                                                                                                                  aCTGACGAATGCGTTGCGTCAAGCGGTAACtggctttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacgcctctgcaaggttctttgctgccgttaacccgtctggtttgggcatgatactgatgtagtcacgctttatcgttttcacgaagctctctgc                       <  1:103729‑M2/251‑222 (MQ=255)                                                                                                                                                                                                                                    ggaCGAATGCGTTGCGTCAAGCGGTAACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacgcctctgcaaggttctttgctgccgttaacccgtctggtttgggcatgatactgatgtagtcacgctttatcgttttcacgaagctctctgcta                     >  2:363958‑M2/2‑28 (MQ=255)                                                                                                                                                                                                                                     ggagattcagggggccagtctaGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCAATCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGc                     >  1:188303‑M1/23‑250 (MQ=255)                                                                                                                                                                                                                                        gAATGCGTTGCGTCAAGCGGTAACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacacctctgcaaggttctttgctgccgttaacccgtctggtttgggcatgatactgatgtagtcacgctttatcgttttcacgaagctctctgctattcc                 >  1:261936‑M2/1‑24 (MQ=255)                                                                                                                                                                                                                                            cagggggccagtctaGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGAtcgt             >  2:85000‑M1/16‑251 (MQ=255)                                                                                                                                                                                                                                             agggggccagtctaGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGAtcgtc            >  1:258124‑M1/15‑251 (MQ=255)                                                                                                                                                                                                                                              gggggccagtctaGTAACCATCCATATTTGGCCTGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATAATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCag                              <  1:139263‑M1/219‑1 (MQ=255)                                                                                                                                                                                                                                              gggggccagtctaGTAACCATCCATATTTGGCATGGTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAATTACATTAAGCGCATCGACGCCATCATTCGCGGTGTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCag                              >  2:139263‑M1/14‑232 (MQ=255)                                                                                                                                                                                                                                                gggccagtctaGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGct         <  1:224226‑M1/240‑1 (MQ=255)                                                                                                                                                                                                                                                       aGCGGTAACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacgcctctgcaaggttctttgctgccgttaacccgtctggtttgggcatgatactgatgtagtcacgctttatcgttttcacgaagctctctgctattccgttactctccggact  <  1:300340‑M2/251‑243 (MQ=255)                                                                                                                                                                                                                                                          aGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCAtc  >  1:27769‑M1/2‑248 (MQ=255)                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                       GCATCTGGCATTTGCACTGAATGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGAATCCCGCGATTTCCTGACCCTCTCGGCATATAACGTCAGCGTCTGTTTTATCACATCCAGCGTTACCGGCTTCGACAGGCAGCTGTCCATACCGGACTCCAGACACCGCTGCTTCTCTTCAGCCAACGCATTAGCAGTTACTCCGATTACCGGCAACGTCAGTCCCAACTGACGAATGCGTTGCGTCAAGCGGTAACCATCCATATTTGGCATGTTGACGTCGCTAAGCACGATATCAATATGATTCTTGCTAAGTACATTAAGCGCATCGACGCCATCATTCGCGGTTTTACATTGATAGCCCAACGATCCCAACTGATCTGCCAGCAAACGCCGGTTAATCGGATGATCATCCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATC  >  NC_000913/2316975‑2317471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.