Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	3,888,720	Δ6 bp	intergenic (+211/‑5)	tnaC → / → tnaA	tnaAB operon leader peptide/tryptophanase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3888719	3 (0.090)	20 (0.590)	18/484	0.7	87.0%	intergenic (+210/‑11)	tnaC/tnaA	tnaAB operon leader peptide/tryptophanase
	?	NC_000913	3888726 =	3 (0.090)					intergenic (+217/‑4)	tnaC/tnaA	tnaAB operon leader peptide/tryptophanase

ATTAGATTCAATGTGATCTATTTGTTTGCTATATCTTAATTTTGCCTTTTGCAAAGGTCATCTCTCGTTTATTTACTTGTTTTAGTAAATGATGGTGCTTGCATATATATCTGGCGAATTAATCGGTATAGCAGATGTAATATTCACAGGGATCACTGTAATTAAAATAAATGAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3888545‑3888719 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aTGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGAGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGATTTACTGACCGACAGCGGCACCGGGGCGGTGACGCAGAGCATGCAGGCTGCGATGATGCGCGGCGACGAAGCCTACAGCGGCAGTCGTAGCTACTATGC  >  NC_000913/3888726‑3888974                                                                                                                                                                                                                                                                                                                                                                                                                                          ATTAGATTCAATGTGATCTATTTGTTTGCTATATCTTAATTTTGCCTTTTGCAAAGGTCCTCTCTCGTTTATTTACTTGTTTTAGTAAATGATGGTGCTTGCATATATATCTGGCGAATTAATCGGTATAGCAGCTGTAATATTCACAGGGATCACTGTAATTAAAATAAATGAATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGAGCCAGTAAAACGTACCACTCGC                                                                                                                                                                               >  1:151611/1‑251      ATTCAATGTGATCTATTTGTTTGCTATATCTTAATTTTGCCTTTTGCAAAGGTCATCTCTCGTTTATTTACTTGTTTTAGTAAATGATGGTGCTTGCATATATATCTGGCGAATTAATCGGTATAGCAGATGTAATATTCACAGGGATCACTGTAATTAAAATAAATGAATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGAGCCAGTAAAACGTACCACTCGCGCTT                                                                                                                                                                           >  1:43751/1‑250          acTGTGGTCTATTTGTTTGCTATGTCTGAATTTTGCCTTTTGCAAAGGTCATCTCTCGTTTATTTACTTGTTTTAGTAAATGATGGTGCTTGCATATATATCTGGCGAATTAATCGGTATAGCAGATGAAATATTCATAGGGATCACTGTAATTAAAATAAATGAATGTAATGGAAAACTTTAAACATATCACTGAACCATTCAGCATTCGTGTTATTGAGCCAGTAACACGTACCACTCGCGATTATCGT                                                                                                                                                                      >  2:271172/3‑251                                         TTTGCCTTTTGCAAAGGTCATCTCTCGTTTATTTACTTGTTTTAGTAAATGATGGTGCTTGCATATATCTCTCGCGAATTAATCGGTATAGCAGATGTAATATTCACAGGGATCACTGTAATTAAAATAAATGAATGTACTGGAAAACTTTAAACATCACCCTGAACCGTTCCGCATTCGTGTTATTGAGCAAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTCTGAACC                                                                                                                                       >  1:39257/1‑251                                                 TTGCATAGGCCATCTCTCGTTTATTTACTTGTTGTAGTAAATGATGGTGCTTGCATATATATCTGGCGAATTAATCGGTAGAGCAGATGTAATATTCACAGGGATCACTGTAATTAAAATAAATGAATGTAATGGAAAACTTTAAACATCTCGCTGAACCTTTCCGCATCCGTGTGATTTAGCCAGTGAAACGGACCTCTCGCGCTTATCGTGAAGACGCATTGATTGAATCCGGTGTGAACCCGTTCCT                                                                                                                                <  2:340718/250‑1                                                       AGGTCATCTCTCGTTTATTTACTTGTTTTAATAAATGATGGTGCTTGCATATATATCTGGCGAATTAATCGGTATAGCAGATGTAATATTCACAGGGATCACTGTAATTAAAATAAATGAATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGAGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGAT                                                                                                                         >  2:103467/1‑251                                                                   GTTTATTTACTTGTTTTAGTAAATGATGGTGCTTGCATATATATCTGGCGAATTAATCGGTATAGCAGATGTAATATTCACAGGGATCACTGTAATTAAAAGAAATGAATTTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGAGCCAGTAAAACGTACCACTCGCGCTTATTGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTGCCTGCTGGATAGCGAAGATGct                                                                                                             <  2:230477/251‑3                                                                      TATTTACTTGTTTTAGTAAATGATGGTGCTTGCATATATATCTGGCGAATTAATCGGTATAGCAGATGTAATATTCACAGGGATCACTGTAATTAAAATAAATGAATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGAGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTT                                                                                                          >  2:182478/1‑251                                                                        TTTACTTGTTTTAGTAAATGATGGTGCTTGAATATATCTCTGGCGAAGTCATCGGTATAGCAGATGTAATATTCACAGGGATCACTGTAATTAAAATAAATGAATGTAATGGAAACCTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGAGCCAGTAACACGTACAACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGCAGATGTTTTTAT                                                                                                        >  1:325093/1‑251                                                                                     aTAAATGATGGTGCTTGCATATATATCTGGCGAATTAATCGGTATAGCAGATGTAATATTCACAGGGATCACTGTAATTAAAATAAATGAATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGAGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGATTTGCTGACC                                                                                           <  1:103467/250‑1                                                                                            ATGGTGCTTGCATATATATCTGGCGAATTAATCGGTATAGCAGCTGTAATATTCACAGTGATCACTGTAATTAAAATAAATGAATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGAGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGATTTACTAACCGACAGCG                                                                                    >  1:122815/1‑251                                                                                                 GCTTGCATATATATCTGGCGAATTAATCGGTATAGCAGATGTAATATTCACAGGGATCACTGTAATTAAAATAAATGAATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGAGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGATTTACTGACCGACAGCGGCACC                                                                               >  2:52320/1‑251                                                                                                               CTGGCGACTTAATCGGGATAGCAGATGTAATATTCACAGGGATCACTGTACTTAAAATAAATGAATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGAGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTCTTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGTTTTACTGACCGACAGCGGCACCGGGGCGGTGACGCc                                                                 >  2:141647/1‑250                                                                                                                 GGCGAATTAATCGGTATAGCAGATGTAATATTCACAGGGATCACTGTAATTAAAATAAATGAATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGAGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTAGCGATTTACTGACCGACAGCGGCACCGGGGCGGTGACGCAGA                                                               <  2:43751/251‑1                                                                                                                             GGTATAGCAGATGTAATATTCACAGGGATCACTGTAATTAAAATAAATGAATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTACTGAGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGATTTACTGACCGACAGCGGCACCGGGGCGGTGACGCAGAGCATGCAGGCTG                                                   <  1:246520/251‑1                                                                                                                                   GCAGAGGTAATCTTCACAGGGATCCCTGTAATTAAAATAAATGAATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGAGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCACTTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGATTTACTGACCGACAGCGGCACCGGGGCGGTGACGCAGAGCATGCAGGCTGCGATG                                              >  2:19779/1‑250                                                                                                                                                   CAGGGATCACTGTAATTAAAATAAATGAATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGAGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGATTTACTGACCGACAGCGGCACCGGGGCGGTGACGCAGAGCATGCAGGCTGCGATGATGCGCGGCGACGAAGC                             >  1:123094/1‑251                                                                                                                                                     GGGATCACTGTAATTAAAATAAATGAATGTAATGGAAAACTTTAAACATCTCCCTGAACAGATCCGCATTAGTGTTATTGAGCCAGTAAAACGTACCACTCGCGCTTATAGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGATTTACTGACCGACAGCGGCACCGGGGCGGTGACGAAAAGCATGCAGGCTGCGATGATGCGCGGAGACGAGGCCT                           >  2:307186/1‑251                                                                                                                                                      GGATCAATGTAATTAAAATAAATGAATGTACTGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGAGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGGATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGATTTACTGACCGACAGCGGCACCGGGGCGGTGACGCAGAGCATGCAGGCCGCGAGGATGCGCGGCGACGAAGCCT                           >  2:113208/1‑250                                                                                                                                                                              AATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGAGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGATTTACTGACCGACAGCGGCACCGGGGCGGTGACGCAGAGCATGCAGGCTGCGATGATGCGCGGCGACGAAGCCTACAGCGGCAGTCGTAGCTACTATGC  >  1:155113/1‑251                                                                                                                                                                                                                                                                                                                                                                                                                                          ATTAGATTCAATGTGATCTATTTGTTTGCTATATCTTAATTTTGCCTTTTGCAAAGGTCATCTCTCGTTTATTTACTTGTTTTAGTAAATGATGGTGCTTGCATATATATCTGGCGAATTAATCGGTATAGCAGATGTAATATTCACAGGGATCACTGTAATTAAAATAAATGAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3888545‑3888719 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aTGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGAGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGATTTACTGACCGACAGCGGCACCGGGGCGGTGACGCAGAGCATGCAGGCTGCGATGATGCGCGGCGACGAAGCCTACAGCGGCAGTCGTAGCTACTATGC  >  NC_000913/3888726‑3888974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.