Strain Name: uap1 / Allele: uy28 |
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| Allele | uy28 |
| Strain Name | uap1 |
| Type | Mutant |
| Genotype | |
| Affected Gene | uap1 |
| Origin and Depositor | University of Yamanashi (Atsuo Kawahara) |
| Link to ZFIN |
| Information | |
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There is a deletion of 9 bp and an insertion of 2 bp (7 bp deletion in total) within uap1 gene as a result of genome editing using the CRISPR/Cas9 system. The deletion results in frame shift and nonsense mutation of the gene and generates a truncated protein with 73 amino acids (wild-type uap1 protein has 504 amino acids). Insertion/deletion region of uap1 gene (small letters indicate insertions, hyphens indicate deletions): wild-type: 5'-CTTCTTTAAGTCGGCCATGAAGACGTCTGGGCAGAGCT-3' mutant: 5'-CTTCTTTAAGTC-------tgGACGTCTGGGCAGAGCT-3' The following primers can be used to detect this mutation: Forward: 5'-AGATGAGTCTCGACCTGGA-3' Reverse: 5'-GTGTCCACCTTCTCCTGTGA-3' This mutation causes the following recessive phenotypes: shorter protrusion of the mouth at 7 dpf and larval death before 14 dpf (no homozygous mutant animal has been found after 14 dpf). This mutant may have a transgene for lyz:EGFP. |
| The Conditions to Distribute Strains | |
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Use of the BIOLOGICAL RESOURCE shall be limited to a collaborative research with the DEPOSITOR. Prior to filing an application for a patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE, the recipient shall acquire the consent from (or notify to) the DEPOSITOR for such application. The BIOLOGICAL RESOURCE shall be used only for basic research purpose (NOT for commercial purpose). |
| Request |
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| Reference | |
| Facility | RIKEN Center for Brain Science |