Strain Name: wnt8aORF2 ; Tg(wnt8aORF1:achilles) / Allele: kt1043 ; kt2011

Allele kt1043 ; kt2011
Strain Name wnt8aORF2 ; Tg(wnt8aORF1:achilles)
Type Mutant, Transgenic
Genotype
Affected Gene wnt8aORF2, wnt8aORF1
Origin and Depositor National Institute for Basic Biology (Shinji Takada)
Link to ZFIN
Information
(Tg(wnt8aORF1:achilles): kt2011)
In this knock-in line, 5xG4S-achilles was into the 3' end of wnt8a ORF1 by CRISPR-mediated homologous recombination.

(wnt8aORF2: kt1043)
7bp deletion as introduced in the exon3 of wnt8a ORF2 by CRISPR method.The mutation was identified by T7 endonuclease assay with primers:
Forward: CGAAGCGTGAGTGTCAAAAC
Reverse: AGAGAGTTGCAGAGCGGTGT
mutation
WT:	TATCTTACCTATGCAAATAGTGTGCGGGTTGGGGCACAGAGTGGGA
kt1043:	TATCTTACCTATGCAAATAGAGTATGCA-------ACAGAGTGGGA

*kt2011 and kt1043 are tightly linked to each other.  
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Reference
 
Facility RIKEN Center for Brain Science