Strain Name: tlx3a / Allele: -11

Allele -11
Strain Name tlx3a
Type Mutant
Genotype
Affected Gene tlx3a
Origin and Depositor Nagoya University (Masahiko Hibi)
Link to ZFIN
Information
A deletion mutation (-11 bp) was introduced in the exon ? of tlx3a gene by a CRISPR method. The mutation can be detected by PCR with the primers:
F:5’- TCGTGTGTTTTGTCTTTACCTGG -3’
R:5’- TCGCTCCCGCCATGATGAG -3’

Mutation:
WT  CCTGTCTTTGGGGAACCTGCGGTTATTGTCCATCCAGGGGAAGCACAGCGCC
MUT CCTGTCTTTGGGGAACCTGCGG--A------A-G--GGGGAAGCACAGCGCC
 
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Reference
 
Facility RIKEN Center for Brain Science