Strain Name: TG (β-actin:EGFP) ; mpv17 / Allele: - ; a9 |
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| Allele | - ; a9 |
| Strain Name | TG (β-actin:EGFP) ; mpv17 |
| Type | Mutant, Transgenic |
| Genotype | |
| Affected Gene | |
| Origin and Depositor | Shizuoka University (Toshinobu Tokumoto) |
| Link to ZFIN |
| Information | |
| Although the cDNA integrated into the line was constructed for the expression of EGFP driven by the medaka β-actin promoter, the expression of EGFP is restricted to the oocytes and gills in adult fish. Germinal vesicles (GV) in oocytes of the established line can be observed by relatively strong fluorescence around the GV. GV migration and GV breakdown (GVBD) could be monitored in real time. Also fluorescence is strong in samll oocytes. That feature enable the observation of small ovary during growth of fish and changing of ovarian size such as sex-change experiment (Takastu et al., Sci. Rep 2013). |
| The Conditions to Distribute Strains | |
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In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Generation of Transparent Zebrafish with Fluorescent Ovaries: A Living Visible Model for Reproductive Biology. Akhter et al., Zebrafish Volume 13, Number 3, p155-160, 2016 |
| Request |
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| Reference | |
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| Facility | RIKEN Center for Brain Science |