Strain Name: Tg(fli1a:LIFEACT-mCherry) ; kdm2ba / Allele: ncv7Tg ; nf7

Allele ncv7Tg ; nf7
Strain Name Tg(fli1a:LIFEACT-mCherry) ; kdm2ba
Type Mutant, Transgenic
Genotype
Affected Gene
Origin and Depositor Nippon Medical School (Shigetomo Fukuhara), National Cerebral and Cardiovascular Center Research Institute (Naoki Mochizuki)
Link to ZFIN
Information
The kdm2banf7 zebrafish mutant was developed using CRISPR/Cas9 technology. The kdm2banf7 mutant carrys a 9-bp deletion and a 2-bp insertion in the exon 4 of kdm2ba, creating a premature stop codon as described below. Wild type and mutant alleles can be determined by BspEI digestion of a PCR product generated with the following primer set: 
5'-CCCAACAAACCCAAACCTAA-3'
and 
5'-AGAAGAATCACTCCCTGCCC-3'
(Wt, 106-bp and 460-bp; Mutant, 566-bp).
The Tg(fli1a:LIFEACT-mCherry)ncv7Tg line has been developed and deposited by Dr. Naoki Mochizuki (National Cerebral and Cardiovascular Center Research Institute).  
ncv7tg_nf7.jpg
The Conditions to Distribute Strains
(nf7)
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, co-authorship of the DEPOSITOR is required.
(ncv7Tg)
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Cdc42 mediates Bmp-induced sprouting angiogenesis through Fmnl3-driven assembly of endothelial filopodia in zebrafish. Wakayama et al. Dev. Cell 2015 Jan 12;32(1):109-122
Prior to filing an application for a patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE, the recipient shall acquire the consent from (or notify to) the DEPOSITOR for such application.  
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Reference

Wakayama Y, Fukuhara S, Ando K, Matsuda M, Mochizuki N. Cdc42 mediates Bmp-induced sprouting angiogenesis through Fmnl3-driven assembly of endothelial filopodia in zebrafish. Dev. Cell. 2015 ; 32(1) : 109-122 .

 
Facility RIKEN Center for Brain Science