Strain Name: Tg(fli1a:LIFEACT-mCherry) ; nostrin / Allele: ncv7Tg ; nf6 |
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| Allele | ncv7Tg ; nf6 |
| Strain Name | Tg(fli1a:LIFEACT-mCherry) ; nostrin |
| Type | Mutant, Transgenic |
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| Affected Gene | |
| Origin and Depositor | Nippon Medical School (Shigetomo Fukuhara), National Cerebral and Cardiovascular Center Research Institute (Naoki Mochizuki) |
| Link to ZFIN |
| Information | |||
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The nostrinnf6 zebrafish mutant was developed using CRISPR/Cas9 technology. The nostrinnf6 mutant carrys large deletion in the nostrin gene (deleting a DNA sequence encoding from exon4 to exon14 of nostrin gene. For wild type allele, 557-bp fragment can be amplified by PCR using the following primer set: 5'-GCGAAAACAACAAGTATTGCAT-3'and 5'-AACATCCGTTCAAGTCCTAGGA-3'For mutant allele, 298-bp fragment can be amplified by PCR using the following primer set: 5'-GCGAAAACAACAAGTATTGCAT-3'and 5'-AACATCCGTTCAAGTCCTAGGA-3' The Tg(fli1a:LIFEACT-mCherry)ncv7Tg line has been developed and deposited by Dr. Naoki Mochizuki (National Cerebral and Cardiovascular Center Research Institute).
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| The Conditions to Distribute Strains | |
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(nf6) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, co-authorship of the DEPOSITOR is required. (ncv7Tg) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cdc42 mediates Bmp-induced sprouting angiogenesis through Fmnl3-driven assembly of endothelial filopodia in zebrafish. Wakayama et al. Dev. Cell 2015 Jan 12;32(1):109-122 Prior to filing an application for a patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE, the recipient shall acquire the consent from (or notify to) the DEPOSITOR for such application. |
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| Facility | RIKEN Center for Brain Science |