Strain Name: kdm1a / Allele: it627

Allele it627
Strain Name kdm1a
Type Mutant
Genotype
Affected Gene kdm1a
Origin and Depositor University of Tsukuba (Makoto Kobayashi)
Link to ZFIN ZDB-ALT-160314-4    
Information
it627 is a mutant strain of kdm1a gene (or LSD1) isolated from an ENU mutagenesis screen. The it627 mutation replaces Gln609 with a stop codon that leads to a truncation of C-terminal ∼200-amino acids including a peptide domain essential for the enzymatic activity. The homozygous mutants have defects in primitive erythropoiesis with a drastic reduction of gata1 expression. They have also defects in a swim bladder inflation and become lethal around 10 dpf larvae.  
The Conditions to Distribute Strains
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Takeuchi et al. (2015) Proc. Natl. Acad. Sci. USA 112:13922-13927
Prior to filing an application for a patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE, the recipient shall acquire the consent from (or notify to) the DEPOSITOR for such application.
The recipient shall treat as confidential and maintain secrecy regarding any information related to the BIOLOGICAL RESOURCE.
The recipient shall disclose all of the research results to be obtained by use of the BIOLOGICAL RESOURCE to the DEPOSITOR.
The BIOLOGICAL RESOURCE shall be used only for basic research purpose (NOT for commercial purpose).  
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Reference

Miki Takeuchi, Yuji Fuse, Mana Watanabe, Christina-Sylvia Andrea, Miho Takeuchi, Hitomi Nakajima, Ken Ohashi, Hiroshi Kaneko, Maki Kobayashi-Osaki, Masayuki Yamamoto, Makoto Kobayashi. LSD1/KDM1A promotes hematopoietic commitment of hemangioblasts through downregulation of Etv2. Proc Natl Acad Sci U S A. 2015 ; 112(45) : 13922-13927 .

 
Facility RIKEN Center for Brain Science