Strain Name: tbx1 / Allele: kt1110

Allele kt1110
Strain Name tbx1
Type Mutant
Genotype
Affected Gene tbx1
Origin and Depositor National Institute for Basic Biology (Shinji Takada)
Link to ZFIN
Information
11base deletion was introduced in exon1 of tbx1 gene by using CRISPR method.
The heterozygous fish was identified by T7-endonuclease assay using followed primers:

pax1a-T7F: CAGCGCAAATTGTAAATCCA
pax1a-T7R: TAATATTGGCTACTTTAGGGTTCTTTT

Mutation:
WT:  AGCAGCCTCAATACGCCCGGGAGTTACC
Mut: AGC-----------GCCCGGGAGTTACC
 
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Reference
 
Facility RIKEN Center for Brain Science