Strain Name: Tg(UAS:hMAMLDN) / Allele: chi10

Allele chi10
Strain Name Tg(UAS:hMAMLDN)
Type Transgenics
Genotype
Affected Gene
Origin and Depositor Chiba University (Motoyuki Itoh)
Link to ZFIN ZDB-ALT-210604-6    
Information
To identify this Tg fish, PCR-based genotyping is required using DNA from embryos or adult fins.

The PCR condions for genotyping are as fellows:
94℃ 2min
(94℃ 30s → 65℃ 30s → 72℃ 65s) x 30cycles

PCR primer sequences are as follows:
UAS Forward	5'-CATCGCGTCTCAGCCTCAC-3'
hMAMLDN Reverse	5'-GTGCAGGGCGAAGGTGTGTT-3'
 
The Conditions to Distribute Strains
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Mizoguchi et al., Development
2020 Aug 27;147(16):dev191312. doi: 10.1242/dev.191312.
Transient activation of the Notch-her15.1 axis plays an important role in the maturation of V2b interneurons  
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Reference

Takamasa Mizoguchi, Michi Fukada, Miku Iihama, Xuehui Song, Shun Fukagawa, Shuhei Kuwabara, Shuhei Omaru, Shin-Ichi Higashijima3, Motoyuki Itoh. Transient activation of the Notch-her15.1 axis plays an important role in the maturation of V2b interneurons. Development. 2020 ; 147(16): : dev191312 .

 
Facility RIKEN Center for Brain Science