Strain Name: Tg(fli1a:LIFEACT-mCherry) ; rap1b / Allele: ncv7Tg ; ncv124

Allele ncv7Tg ; ncv124
Strain Name Tg(fli1a:LIFEACT-mCherry) ; rap1b
Type Mutant, Transgenic
Genotype
Affected Gene
Origin and Depositor National Cerebral and Cardiovascular Center (Naoki Mochizuki)
Link to ZFIN ZDB-ALT-150603-1    
Information
(ncv7Tg)
This Tg line expresses C-terminally mCherry-tagged LIFEACT, a small actin binding peptide, under the control of endothelial-specific fli1a promoter. LIFEACT-mCherry indicates localization of F-actin.
(ncv124)
The rap1bncv124 mutant, which was developed using TALEN technique, harbors an 10-bp deletion in the exon 3 of rap1b, resulting in a premature stop codon. Mutant alleles can be identified by BbsI digestion of a PCR product generated with the following primer set: 5'-tgtactgtgtgcgtgtgttg-3' and 5'-tcctgaccagcaagtgtagg-3'. The Tg(fli1a:LIFEACT-mCherry)ncv7Tg line has already been deposited separately.  
wt-rap1bncv124-rap1bncv125.jpg
The Conditions to Distribute Strains
(ncv7Tg)
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Cdc42 mediates Bmp-induced sprouting angiogenesis through Fmnl3-driven assembly of endothelial filopodia in zebrafish. Wakayama et al. Dev. Cell 2015 Jan 12;32(1):109-122
Prior to filing an application for a patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE, the recipient shall acquire the consent from (or notify to) the DEPOSITOR for such application.
(ncv124)
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Rho S., Kobayashi I., Oguri-Nakamura E., Ando K., Fujiwara M., Kamimura N., Hirata H., Iida A., Iwai Y., Mochizuki N., Fukuhara S. Rap1b Promotes Notch-Signal-Mediated Hematopoietic Stem Cell Development by Enhancing Integrin-Mediated Cell Adhesion. Developmental Cell 49(5): 681-696.e6(2019)
Prior to filing an application for a patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE, the recipient shall acquire the consent from the DEPOSITOR for such application.
The BIOLOGICAL RESOURCE shall be used only for basic research purpose (NOT for commercial purpose).
The recipient and the DEPOSITOR shall sign an MTA, which set forth the terms and conditions to use the intellectual property rights based on the results of research using the BIOLOGICAL RESOURCE and so on. The recipient shall be sent an agreement for distribution obtained from the DEPOSITOR to NZC.  
Request To Order
Reference

Rho SS, Kobayashi I, Oguri-Nakamura E, Ando K, Fujiwara M, Kamimura N, Hirata H, Iida A, Iwai Y, Mochizuki N, Fukuhara S. Rap1b Promotes Notch-Signal-Mediated Hematopoietic Stem Cell Development by Enhancing Integrin-Mediated Cell Adhesion. Dev. Cell. 2019 ; 49(5) : 681-696.e6 .

Wakayama Y, Fukuhara S, Ando K, Matsuda M, Mochizuki N. Cdc42 mediates Bmp-induced sprouting angiogenesis through Fmnl3-driven assembly of endothelial filopodia in zebrafish. Dev. Cell. 2015 ; 32(1) : 109-122 .

 
Facility RIKEN Center for Brain Science