Strain Name: Tg(fli1a:Myr-EGFP) ; Tg(fli1a:LIFEACT-mCherry) ; kctd10 / Allele: ncv2Tg ; ncv7Tg ; nf1m

Allele ncv2Tg ; ncv7Tg ; nf1m
Strain Name Tg(fli1a:Myr-EGFP) ; Tg(fli1a:LIFEACT-mCherry) ; kctd10
Type Mutant, Transgenic
Genotype
Affected Gene kctd10
Origin and Depositor National Cerebral and Cardiovascular Center (Naoki Mochizuki) ; Nippon Medical School (Shigetomo Fukuhara)
Link to ZFIN ZDB-ALT-150113-1     ZDB-ALT-150603-1    
Information
(ncv2Tg) This Tg line expresses myristoylation signal (Myr)-tagged EGFP under the control of endothelial cell-specific fli1a promoter. Thus, the plasma membrane of endothelial cells can be labeled by EGFP fluorescence in this Tg line.
(ncv7Tg) This Tg line expresses C-terminally mCherry-tagged LIFEACT, a small actin binding peptide, under the control of endothelial-specific fli1a promoter. LIFEACT-mCherry indicates localization of F-actin.
(nf1m) The kctd10nf1m mutant, which was developed using TALEN technique, harbors a 5-bp deletion and a 1-bp insertion in the exon 3 of kctd10, resulting in a premature stop codon as described below. Mutant alleles can be identified by HpaII digestion of a PCR product generated with the following primer set: 5'-CCTCACTGATAGCGAAGGTAGG-3' and 5'-CATTTTGGTGCGATTATTACCC-3'.  
kctd10-nf1.jpg
The Conditions to Distribute Strains
(ncv2Tg)
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Fukuhara S, Zhang J, Yuge S, Ando K, Wakayama Y, Sakaue-Sawano A, Miyawaki A, Mochizuki N. Visualizing the cell-cycle progression of endothelial cells in zebrafish. Dev.Biol. 2014 393(1):10-23
(ncv7Tg)
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Cdc42 mediates Bmp-induced sprouting angiogenesis through Fmnl3-driven assembly of endothelial filopodia in zebrafish. Wakayama et al. Dev. Cell 2015 Jan 12;32(1):109-122
Prior to filing an application for a patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE, the recipient shall acquire the consent from (or notify to) the DEPOSITOR for such application.
(nf1m)
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, co-authorship of the DEPOSITOR is required.  
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Reference

Wakayama Y, Fukuhara S, Ando K, Matsuda M, Mochizuki N. Cdc42 mediates Bmp-induced sprouting angiogenesis through Fmnl3-driven assembly of endothelial filopodia in zebrafish. Dev. Cell. 2015 ; 32(1) : 109-122 .

Fukuhara S, Zhang J, Yuge S, Ando K, Wakayama Y, Sakaue-Sawano A, Miyawaki A, Mochizuki N. Visualizing the cell-cycle progression of endothelial cells in zebrafish. Dev. Biol. 2014 ; 393(1) : 10-23 .

 
Facility RIKEN Center for Brain Science