Strain Name: six7; Tg(-5.2crx:EGFP-2A-six7-FLAG) / Allele: ja51; ja69Tg |
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| Allele | ja51; ja69Tg |
| Strain Name | six7; Tg(-5.2crx:EGFP-2A-six7-FLAG) |
| Type | Mutant, Transgenic |
| Genotype | |
| Affected Gene | |
| Origin and Depositor | The University of Tokyo (Yoshitaka Fukada) |
| Link to ZFIN |
| Information | |
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(ja51) Genotypes were determined by a combination of PCR and subsequent digestion by endonuclease XhoI. First, the region flanking the TALEN cleavage site was amplified by PCR using the following primers, six7TALEN_Fw (5’- TCTGCTGCTCCTCCTTTAC -3’) and six7TALEN_Rv (5’- CTTACGGATGCGGTACTTC -3’). Second, the PCR product was digested with XhoI, and TALEN-induced mutations were checked by loss of the XhoI digestion site. (ja69Tg) The transgenes, EGFP and Six7-FLAG, are fused to the promoter region of cone-rod homeobox (crx) gene. In this transgenic background, both EGFP and Six7-FLAG are specifically expressed in the retinal and pineal photoreceptor cells. |
| The Conditions to Distribute Strains | |
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In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Ogawa Y, Shiraki T, Kojima D, Fukada Y. (2015) Homeobox transcription factor Six7 governs expression of green opsin genes in zebrafish. Proc. R. Soc. B, 282, 20150659. Ogawa Y, et al. (2019) Six6 and Six7 coordinately regulate expression of middle-wavelength opsins in zebrafish. Proc Natl Acad Sci U S A:201812884. The BIOLOGICAL RESOURCE shall be used only for basic research purpose (NOT for commercial purpose). |
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| Facility | RIKEN Center for Brain Science |