Strain Name: Tg(5×UAS:EGFP-P2A-krasG12D) ; Tg(fabp10a:mCherry) ; igfbp1a[-53] |
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| Allele | |
| Strain Name | Tg(5×UAS:EGFP-P2A-krasG12D) ; Tg(fabp10a:mCherry) ; igfbp1a[-53] |
| Type | Mutant, Transgenic |
| Genotype | |
| Affected Gene | |
| Origin and Depositor | Advanced Telecommunications Research Institute International [ATR] (Shinpei Kawaoka) |
| Link to ZFIN |
| Information | |
| Tg(5×UAS:EGFP-P2A-krasG12D) is expressed in the presence of a Gal4 gene. krasG12G gene is linked to EGFP by a P2A sequence. This transgene can be detected by a genomic PCR using Fwd(5′-CCACCTACGGCAAGCTGACC-3′) and Rev(5′-tcccacgaccacaagcttat-3′) primers. The product size is 701 bp. Tg(fabp10a:mCherry) is expressed in the liver under the control of a fabp10a promoter from 3 dpf. The 53 bp deletion-mutation in the igfbp1a locus is introduced by the CRISPR/Cas9 system. This mutation is detectable by a genomic PCR using Fwd(5′-GTGGCAGCATTCAGCGCACTCCTC-3′) and Rev(5′-GGCATCCACCGCCGGACACTC-3′) primers. |
| The Conditions to Distribute Strains | |
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In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, co-authorship of the DEPOSITOR is required. Prior to filing an application for a patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE, the recipient shall acquire the consent from (or notify to) the DEPOSITOR for such application. |
| Request |
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| Reference | |
| Facility | RIKEN Center for Brain Science |