Strain Name: rras / Allele: SO1

Allele SO1
Strain Name rras
Type Mutant
Genotype
Affected Gene rras
Origin and Depositor Musashino University (Shinya Ohata)
Link to ZFIN
Information
In the exon3 of rras, two bases (GA) are replaced by three bases (AAA), resulting in a premature termination codon. For genotyping PCR, use the following primers: rras F, 5'- GTA GTA ATT ATT ACA GTG AGT GTG -3'; rrasSO1 WT R, 5'- CTG TCT AAT CGT GTC TCC TTC C -3'; rrasSO1 mutant R, 5'- CTG TCT AAT CGT GTC TCC TTA AAC -3'.  
The Conditions to Distribute Strains
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Ohata S, Uga H, Okamoto H, Katada T.　"Small GTPase R-Ras participates in neural tube formation in zebrafish embryonic spinal cord." Biochem Biophys Res Commun. 2018 Jun 27;501(3):786-790. doi: 10.1016/j.bbrc.2018.05.074.  
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Reference

Ohata S, Uga H, Okamoto H, Katada T. Small GTPase R-Ras participates in neural tube formation in zebrafish embryonic spinal cord. Biochem Biophys Res Commun. 2018 ; 501(3) : 786-790 .

 
Facility RIKEN Center for Brain Science