Strain Name: rras / Allele: SO1
|Origin and Depositor||Musashino University (Shinya Ohata)|
|Link to ZFIN|
|In the exon3 of rras, two bases (GA) are replaced by three bases (AAA), resulting in a premature termination codon. For genotyping PCR, use the following primers: rras F, 5'- GTA GTA ATT ATT ACA GTG AGT GTG -3'; rrasSO1 WT R, 5'- CTG TCT AAT CGT GTC TCC TTC C -3'; rrasSO1 mutant R, 5'- CTG TCT AAT CGT GTC TCC TTA AAC -3'.|
|The Conditions to Distribute Strains|
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Ohata S, Uga H, Okamoto H, Katada T. "Small GTPase R-Ras participates in neural tube formation in zebrafish embryonic spinal cord." Biochem Biophys Res Commun. 2018 Jun 27;501(3):786-790. doi: 10.1016/j.bbrc.2018.05.074.
|Facility||RIKEN (Wako) CBS, Lab for Neural Circuit Dynamics of Decision Making|