Strain Name: Tg(8xGliBS-miniP:d2EGFP) / Allele: isi02

Allele isi02
Strain Name Tg(8xGliBS-miniP:d2EGFP)
Type Transgenics
Genotype
Affected Gene
Origin and Depositor Kyushu University (Tohru Ishitani) Present affiliation: Osaka University
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Information
The Hedgehog (Hh)-signaling pathway is essential for development and adult tissue homeostasis in vertebrates. Binding of Hh ligands to the transmenbrane receptor relieves inhibition of Smoothened (Smo), and then promotes Hh target genes expression by the Gli transcription factors. This transgenic line carries 8xGliBS-miniP:d2EGFP reporter, which expresses a destabilized enhanced green fluorescent protein (d2EGFP) in response to Hh-signaling activation. The d2EGFP expression pattern becomes readily apparent in the embryonic forebrain within the midline axis, which consistent with shh expression stages. And, d2EGFP is observed in forebrain/midbrain boundary, floor plate, somitic myotome, fin bud and swim bladder. Importantly, this expression is abolished upon the treatment with Smo antagonist cyclopamine. Using this line, it is powerful tool for studying Hh-signaling dependent processes during embryogenesis and rapidly screening high-potency small molecule inhibitors of Hh-signaling pathway in vertebrates.  
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The Conditions to Distribute Strains
Use of the BIOLOGICAL RESOURCE shall be limited to a collaborative research with the DEPOSITOR.
Prior to filing an application for a patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE, the recipient shall acquire the consent from (or notify to) the DEPOSITOR for such application.
The recipient shall disclose all of the research results to be obtained by use of the BIOLOGICAL RESOURCE to the DEPOSITOR.
Prior to publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, the recipient shall acquire the consent from the DEPOSITOR.  
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Reference
 
Facility RIKEN Center for Brain Science