Information
- Credit card payments are currently not available at RIKEN and National Institutes of Natural Sciences. Bank transfers are available.
- News letter Mar2026 was distributed.(News letter)
- Announcement of NIG-Zebra Release
We are pleased to announce that a new version of the database for the gene trap and enhancer trap lines from the National Institute of Genetics (NIG) is now accessible through NIG-Zebra. Expression data images for each line are also available in their detailed information pages. We plan to continue adding new lines in the future. - The price list (National Institute of Genetics) was updated on April 1st 2025. Click 「here」for new price list.
- A zebrafish inbred strain, Mishima-AB (M-AB) was established. The link to the summary is here.
- A revolutionary method for in vitro storage of zebrafish and medaka sperm at room temperature has been published. Using this method, it is possible to ship the sperm suspension in a 0.5 ml tube in an envelope (PDF download). The link to the summary is here.
- The price list (RIKEN Center for Brain Science) was updated on April 1st 2023. Click 「 here 」for new price list.
In view of the rising cost of goods and labor, it has become necessary to raise prices. This revision has been approved by Steering Committee. Thank you for your understanding.
Strain Information
Dr. Makoto Kobayashi (University of Tsukuba)
Tg (-3.5gstp1:GFP)it416b
A transgenic line harboring the GFP reporter gene fused to the 3.5-kbp promoter region of zebrafish gstp1 gene and the Tol2 transposon elements.
In the larvae, strong GFP induction is observed in the olfactory regions upon treatment of electrophiles or oxidative stress that activate transcription factor Nrf2, such as auranofin, cadmium chloride, 15-deoxy-Δ12,14-prostaglandin J2, diethylmaleate, hydrogen peroxide and nitro-oleic acid.
In the larvae, constitutive GFP expression is detected in the lens, ears, fins, pericardium and lateral lines.
In the embryos genereted from heterozygous transgenic males and non-transgenic females, no constitutive GFP expression is observed. In the embryos generated from transgenic females, ubiquitous maternal GFP expression is observed.
Viability: Adult-viable.
Dr. Tohru Ishitani (Kyusyu University)
Tg (6xTcf/LefBS-miniP:d2EGFP)isi01
The Wnt/β-catenin signaling pathway plays a crucial role in the development and maintenance of multiple vertebrate organ systems. Binding of Wnt ligands to the receptor complexes on the cell surface induces the accumulation of cytoplasmic β-catenin. Accumulated β-catenin enters into the nucleus and then binds to Tcf/Lef transcription factor to activate the target gene expression. This transgenic line carries 6xTcf/LefBS-miniP:d2EGFP reporter, which expresses a destabilized enhanced green fluorescent protein (d2EGFP) in response to Wnt/β-catenin signaling activation. The expression of this reporter is obserbed in tectum, MHB, optic vesicle, retina, anterior-posterior lateral line primordium, fin bud, and caudal fin etc. Though the reporter gene is multiply inserted to the genome in this version of transgenic line, this line is a highly useful tool for studying Wnt/β-catenin signaling dependent processes during embryogenesis and regeneration.
Dr. Atsushi Miyawaki (RKEN BSI)
Tg(EF1α:mKO2-zCdt1(1/190)) / rw0405b
Tg(EF1α:mKO2-zCdt1(1/190)) / rw0405d
Tg(EF1α:mAG-zGem(1/100)) / rw0410h
Tg(EF1α:mAG-hGem(1/60)) / rw0412a
Illuminating Cell-Cycle Progression in the Developing Zebrafish Embryo. zFucci provides sweeping views of cell proliferation in whole fish embryos.
Dr. Kawamura (University of Tokyo)
Tg (zfSWS1-5.5A:EGFP)tkk002 Information
Dr. Kawamura (University of Tokyo)
Tg (zfRH1-3.7B:EGFP)tkk001 Information
Dr. Yoshihara (RIKEN Brain Science Institute)
Mutually exclusive glomerular innervation by two distinct types of olfactory sensory neurons revealed in transgenic zebrafish. Information
Dr. Higashijima
(National Institutes of Natural Sciences, Okazaki Institute for Integrative Bioscience)
alx, a zebrafish homolog of Chx10, marks ipsilateral descending excitatory interneurons that participate in the regulation of spinal locomotor circuits.
Tg(chx10:GFP), Tg(chx10:loxP-DsRed-loxP-GFP), Tg(chx10:Kaede) Information
Dr. Sato
(RIKEN Brain Science Institute)
HuC:Kaede Information
