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遺伝子組換えゼブラフィッシュについて

遺伝子組換えゼブラフィッシュ拡散防止措置の例(PDFダウンロード

運営会議議事録

系統の紹介

小林麻己人先生

Tg (-3.5gstp1:GFP)it416b  

A transgenic line harboring the GFP reporter gene fused to the 3.5-kbp promoter region of zebrafish gstp1 gene and the Tol2 transposon elements.
In the larvae, strong GFP induction is observed in the olfactory regions upon treatment of electrophiles or oxidative stress that activate transcription factor Nrf2, such as auranofin, cadmium chloride, 15-deoxy-Δ12,14-prostaglandin J2, diethylmaleate, hydrogen peroxide and nitro-oleic acid.
In the larvae, constitutive GFP expression is detected in the lens, ears, fins, pericardium and lateral lines.
In the embryos genereted from heterozygous transgenic males and non-transgenic females, no constitutive GFP expression is observed. In the embryos generated from transgenic females, ubiquitous maternal GFP expression is observed.
Viability: Adult-viable.

 

石谷太先生

系統名:Tg (6xTcf/LefBS-miniP:d2EGFP)isi01  

The Wnt/β-catenin signaling pathway plays a crucial role in the development and maintenance of multiple vertebrate organ systems. Binding of Wnt ligands to the receptor complexes on the cell surface induces the accumulation of cytoplasmic β-catenin. Accumulated β-catenin enters into the nucleus and then binds to Tcf/Lef transcription factor to activate the target gene expression. This transgenic line carries 6xTcf/LefBS-miniP:d2EGFP reporter, which expresses a destabilized enhanced green fluorescent protein (d2EGFP) in response to Wnt/β-catenin signaling activation. The expression of this reporter is obserbed in tectum, MHB, optic vesicle, retina, anterior-posterior lateral line primordium, fin bud, and caudal fin etc. Though the reporter gene is multiply inserted to the genome in this version of transgenic line, this line is a highly useful tool for studying Wnt/β-catenin signaling dependent processes during embryogenesis and regeneration.

宮脇敦史先生

魚の胚発生における増殖と分化のパターンが生きたまま丸見え

-ゼブラフィッシュで機能する蛍光性細胞周期の可視化プローブzFucciの開発-

系統名:
Tg(EF1α:mKO2-zCdt1(1/190)) / rw0405b
Tg(EF1α:mKO2-zCdt1(1/190)) / rw0405d
Tg(EF1α:mAG-zGem(1/100)) / rw0410h
Tg(EF1α:mAG-hGem(1/60)) / rw0412a          情報  


河村正二先生

系統名:Tg (zfSWS1-5.5A:EGFP)tkk002   情報

河村正二先生

系統名:Tg (zfRH1-3.7B:EGFP)tkk001   情報

吉原良浩先生

系統名:OMP:CFP, TRPC2:YFPを用いた嗅覚神経回路の解析   情報


東島眞一先生

系統名:Tg(chx10:GFP), Tg(chx10:loxP-DsRed-loxP-GFP), Tg(chx10:Kaede)   情報

佐藤智美先生

系統名:HuC:Kaede   情報

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