Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,089,711	C→T	intergenic (+33/‑44)	galP → / → yggI	galactose:H(+) symporter/protein YggI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,089,711	0	C	T	100.0%	24.9 / NA	10	intergenic (+33/‑44)	galP/yggI	galactose:H(+) symporter/protein YggI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/3);  total (7/3)

CAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCAACACGCTGGGTAACGCCAACACCTTCTGGGTGTATGCGGCTCTGAACGTACTGTTTATCCTGCTGACATTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAAGCTTCCTCCCATCGCGGAGGAAGCCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGTCTCCCTATCGCCATCCAACAGGCCGTTATGCGTCGCCTGCGGGAAAAACTCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGGCTGGAAAGCTATGAAATTCGCCTCAAT  >  NC_000913/3089474‑3089934                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                cAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCAACACGCTGGGAACCGCCAAAACCTTCTGGGAGTATGCAGCTCTGCACGTACTGTTTATCCTGCTGACCTTGAGGCTGGTCCCGGAAACCAAACACGTTTAGCTGGACCATATTGAACGTAATCTGATGCAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCACCAAGCTTCCTCCCATCGCGGAGGAAGTCACCTCTTGCAGt                                                                                                                                                                                                                    >  1:74759/1‑251 (MQ=255)                                                                             nctctGAACGTACTGTTTATCCTGCTGACATTGTGGCTGGTACCGGAACCCAAACAAGTTTCGCAGGAACATATTGAACCTAATCTGATGAACGGACGTACACTGCGCGAAATAGGCGCTCCCGATTAATCTCCCCAAGCCTCCTCCCATCGCGGAGGAAGTCACCTCTTGCAGTCATCTTTTCTTCCCTCTATCCTCTGCCGCTATGAAAACATCCCGTCTCCCTATCGCCACCCAACAGGCCGTTATGc                                                                                                                                        >  1:18846/2‑251 (MQ=255)                                                                                                         cATTGTGGCTGGTACCGGAAACCAAACCCGTTTCGCTGGAACATATTGAACGTAATCTGAGGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAAGCTTCCTCCCATCGCGGAGGAAGTCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGTCTCCCTATCGCCATCCAACAGGCCGTTATGCGTCGCCTGCGGGAAAAACTCGCCCAggn                                                                                                            <  1:95745/251‑2 (MQ=255)                                                                                                                   ggTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGTTCGTACACTGAGCGAAATAGGCGCTCACGATTAATCTCCCCAAGCTTCCTCCCATCGCGGAGGAAGTCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATcc                                                                                                                                                                          >  1:109466/1‑179 (MQ=255)                                                                                                                   ggTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTANTCTNCCCANGCNTCCTCTCATCGCGGANGAAGTCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCGCTGCNNCNNNGNAAACATcc                                                                                                                                                                          <  2:109466/179‑1 (MQ=255)                                                                                                                                                                                         gAAATAGGCGCTCACGATTAATCTCCCCAAGCTTCCTCCCATCGCGGAGGAAGTCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGTCTCCCTATCGCCATCAAACAGACCGTTATGCGTCGCCTGCGGGAAAAACTCGCCCAGGCCAACCTGAAGCTAg                                                                                              >  1:92642/1‑185 (MQ=255)                                                                                                                                                                                             tAGGCNCTCACNANNAATCTCCCCAAGCTTCCTCNCATCGCGGAGGAAGTNACCTNTTGCANTCNt                                                                                                                                                                                                                 >  2:60452/1‑66 (MQ=255)                                                                                                                                                                                             tAGGCGCTCACGATTAATCTCCCCAAGCTTCCTCCCATCGCGGAGGAAGTCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGTCTCCCTATCGCCATCCAACAGGCCGTTATGCGTCGCCTGCGGGAAAAACTCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTac                                                              <  1:60452/213‑1 (MQ=255)                                                                                                                                                                                                                 ccccAAGCTTCCTCCCATCGCGGAGGAAGTCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGTCTCCCTATCGCCATCCAACAGGCCGTTATGCGTCGCCTGCGGGAAAAACTCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGGCTGGAAAGCTATGAAATTCGCCTc     >  1:44696/1‑250 (MQ=255)                                                                                                                                                                                                                   ncAAGCTTCCTCCCATCGCGGAGGAAGTCACCTCTTGCCGTCATCTTTTATTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGTCTCCCTATCGCCATCCAACAGGCCGTTATGCGTCGCCTGCGGGAAAAACTCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGGCTGGAAAGCTATGAAATTCGCCTCAAt  >  1:32007/2‑251 (MQ=255)                                                                                                                                                                                                                                            aGNCACNTNNGNNAGTNATCTTTTCTGCGCCCTATCCTNNGCCGCTATANAAACATCCCGTCTCCCTATCGCCATCCAACAGGCCGTTATGCGTCGCCTGCGGNAAAAACTCGNNCANGCCACNCNNNANNNAGNGCGTAACTACCCGGATCCANAANNCTCTTANTCCCAGCGCGGACCCGCCGCCGGAAc                                    <  2:33269/192‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                CAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCAACACGCTGGGTAACGCCAACACCTTCTGGGTGTATGCGGCTCTGAACGTACTGTTTATCCTGCTGACATTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAAGCTTCCTCCCATCGCGGAGGAAGCCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGTCTCCCTATCGCCATCCAACAGGCCGTTATGCGTCGCCTGCGGGAAAAACTCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGGCTGGAAAGCTATGAAATTCGCCTCAAT  >  NC_000913/3089474‑3089934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑