Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,089,711	C→T	intergenic (+33/‑44)	galP → / → yggI	galactose:H(+) symporter/protein YggI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,089,711	0	C	T	100.0%	34.3 / NA	16	intergenic (+33/‑44)	galP/yggI	galactose:H(+) symporter/protein YggI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/9);  total (7/9)

AACGCCAACACCTTCTGGGTGTATGCGGCTCTGAACGTACTGTTTATCCTGCTGACATTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAAGCTTCCTCCCATCGCGGAGGAAGCCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGTCTCCCTATCGCCATCCAACAGGCCGTTATGCGTCGCCTGCGGGAAAAACTCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGGCTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTG  >  NC_000913/3089523‑3089949                                                                                                                                                                                             |                                                                                                                                                                                                                                               aaCGCCAACCCCTTCTGGGTGTATGCGGCTCTGAACGTACTGTTTATCCTGCTGACATTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAAGCTTCCTCCCACCGCGGAGGAAGTCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGTCTcc                                                                                                                                                                                  <  1:107887/251‑1 (MQ=255)       nacncCTNCNNGNNGTANGCGGCTCTGAACGTACTGTTTNNCCTTCTGACNTTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAANCTGATGAAANNTCNTAAACNGNNNGNNNTANGCGCTCACGATTAATCTCCNCANNCTTCCTNCCATCGCGGAGGAAGTCACCTCTTGc                                                                                                                                                                                                                                      <  2:49141/192‑1 (MQ=255)       aaCACCTTCTGGGTGTATGCGGTTCTGAACGTACTGTTTATCCTGCTGACATGGTGGCTGGTACCTGAAACCAAACACGTTTCGCTGGAACATATTTAACGTAATCTGATTAAATGTCGTAAACTGCGCGAAATAGGCTCTCACGATTAATCTCCCCAAGCTTCCTCCCATCTCCGAGGAAGTCTCCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATcac                                                                                                                                                                                        <  1:218487/239‑3 (MQ=255)              tCTGGGTGTATGCGGCTCTGAACGTACTGTTTATCCTGCTGACATTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATATGCGCTCACGATTAATCTCCCCAAGCTTCCTCCCATCGCGGAGGAAGTCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGTCTCCCTATCGCCATc                                                                                                                                                                       <  1:36925/249‑1 (MQ=255)                            gCTCTGAACGTACTGTTTATCCTGCTGACATTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAAGCTTCCTCCCATCGCGGAGGAAGTCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGTCTCCCTATCGCCATCCAACAGGCCGTTATGc                                                                                                                                                       >  1:38211/1‑251 (MQ=255)                                                      gACATTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACAGATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAGTCTCCCCAAGCTTCCTCCCATCGCGGAGGAAGTCACCTCTGGCAGTCATCTTTTCTTCCCTCTATCCTCTGCCGCTATGAAAACATCCCGTCTCCCTACCGCCATCCAACAGGCCGTTATGCGTCGCCTGCGGGAAAAACTCGCCCAg                                                                                                                             <  1:225966/251‑1 (MQ=255)                                                                      ccGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGACAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAAGCTTCCTCCCATCGCGGAGGAAGTCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCctc                                                                                                                                                                                                            >  1:189657/1‑156 (MQ=255)                                                                                                                            cGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAAGCTTCCTCCCATCGCGGAGGAAGTCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGTCTCCCTATCNCCANNNNNc                                                                                                                                                                   >  2:139439/1‑143 (MQ=255)                                                                                                                             ntAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAAGATTCCTCCCATCGCGGAGGACGTCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCct                                                                                                                                                                                                             >  1:45742/2‑100 (MQ=255)                                                                                                                                       cgAAATAGGCGCTCACGATTAATCTCCCCAAGCTTCCTCCCATCGCGGAGGAAGTCACCTCTTGCAGACACCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGTCTCCCTATCGCCATCCAACAGGc                                                                                                                                                               <  1:224446/136‑1 (MQ=255)                                                                                                                                       cgAAATAGGCGCTCACGATTAATATCCCCAAGCTTCCTCCCATCGCGGAGGAAGTCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGTCTCCCTATCGCCATCCAACAGGCCGTTATGCGTCGCCTGCGGGAAAAACTCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAAc                                                   >  1:65684/1‑244 (MQ=255)                                                                                                                                                              ctcCCCCAGCTTCCTCCCATCGCGGCGGCAGTCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGTCTCCCTATCGCCATCCAACAGGCCGTTATGCGTCGCCTGCGGGAAAAACTCGCCCAGGCGAACCTGAAGCTAGGGCGTAACTACCCGGAGCCCAACCTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGGCTGGAAAGCTATGAAATTAGCCt                     >  1:64147/1‑251 (MQ=255)                                                                                                                                                                             cccATCGCGGAGGAAGTCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGTCTCCCTATCGCCATCCAACAGGCCGTTATGCGTCGCCTGCGGGAAAAACTCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGGCTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTgn       <  1:59110/250‑2 (MQ=255)                                                                                                                                                                                  cgcgGAGGAAGTCACCTCTTGCAGTCATCTTTGCTTCGCTCTATCCTCTGCCGCTATGAAAACAGCCCGTCCCCCTGTCGCCATCCAACAGGCCGTTATGCGTCGCCTGCGGGAAAAACTCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTTCCCGGAGCCAAAACTCGCTTACTCCCAGCGCGGAACCTCCTCCGGAACGGCCTGGCTAGAAAGCTATGAAATTCGCCGCAATCCCGTTTTGCTGTTg  <  1:147594/250‑1 (MQ=255)                                                                                                                                                                                    cgGANNAAGTCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGTCTCCCTATCGCCATCCAACAGGCCGt                                                                                                                                                            <  2:18107/94‑1 (MQ=255)                                                                                                                                                                                    cgGAGGAAGTCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGTCTCCCTATCGCCATCCAACAGGCCGt                                                                                                                                                            >  1:18107/1‑94 (MQ=255)                                                                                                                                                                                             |                                                                                                                                                                                                                                               AACGCCAACACCTTCTGGGTGTATGCGGCTCTGAACGTACTGTTTATCCTGCTGACATTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAAGCTTCCTCCCATCGCGGAGGAAGCCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGTCTCCCTATCGCCATCCAACAGGCCGTTATGCGTCGCCTGCGGGAAAAACTCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGGCTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTG  >  NC_000913/3089523‑3089949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑