Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,429,708	0	A	G	58.8%	7.8 / 10.0	17	T220T (ACA→ACG)	stfR	putative prophage side tail fiber protein StfR
Reads supporting (aligned to +/- strand):  ref base A (5/2);  new base G (7/3);  total (12/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.21e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AACCCATGCGGCTGATGCTGCGGACTCAGCACGCGCAGCCAGCACGTCAGCCGGACAGGCCGCGTCGTCGGCTCAGTCAGCGTCTTCCAGCGCAGGAACGGCATCAACAAAGGCCACTGAAGCATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCAGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCAAAAGAAGCGGCAAAATCATCAGAAACGAACGCATCATCAAGCGCCAGTAGTGCAGCTTCCTCGGCAACGGCGGCAGGAAATTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAA  >  NC_000913/1429474‑1429944                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                             ggcccttgtgactgatgcaactgACTCAGCACGCGCCGCCAGCACGTCCGCCGGACAGGCTGCATCGTCAGCTCAGGAAGCGTCCTCCGGCGCAGAAGCGGCATCAGCAAAGGCCACTGAAGCGGAAAAAAGTGCCGCAGCCGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAAGCAGCCGCCACGTCTGCCTCCACcgcgg                                                                                                                                                                                                                              <  1:79235/229‑2 (MQ=255)   cccttgtgactgatgcaactgACTCAGCACGCGCCGCCAGCACGTCCGCCGGACAGGCTGCATCGTCAGCTCAGGAAGCGTCCTCCGGCGCAGAAGCGGCATCAGCAAAGGCCACTGAAGCGGAAAAAAGAGCCGCAGCCGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACcgcggcc                                                                                                                                                                                                                            >  1:17508/21‑248 (MQ=255)                                ctcgccgccagcACGTCCGCCGGACAGGCTGCATCGTCAGCTCAGGAAGAGTCCTCCGGCGCAGAAGCGGCATCAGCAAAGGCCACTGAAGCGGAAAAAAGTGCCGCAGCCGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGCATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGaggccgccacttcagc                                                                                                                                                                                               >  1:224908/7‑236 (MQ=255)                                       ccagcACGTCAGCCGGACAGGCCGCGTCGTCGGCTCAGTCAGCGTCTTCCAGCGCAGGAACCGAATCAACACAGGCCACTGAAGCATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCAGCGGCCTCCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCAGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGCCCTCGGCCCGGGAt                                                                                                                                                                                        >  1:229482/1‑251 (MQ=255)                                                                                  gTCTTCCAGCGCAGGAACGGAATCAACAAAGGCCAATGAAGCATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCAGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGACGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCAAAAGAAGCGGCAAAATCATCAGAaacgaacg                                                                                                                                             >  1:198218/1‑251 (MQ=255)                                                                                               naagcGGCATCAGCAAAGGCCACTGAAGCGGAAAAAAGTGCCGCAGCCGCAGAGTCATCAAAAAACGCAGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGCGATGCGGTGGCCTCAAAAGAGACAGCAAAATCATCAGCAACGAACGCATCATCAAgtgc                                                                                                                                >  1:24836/5‑248 (MQ=255)                                                                                               gaagcGGCATCAGCAACGGCCACTGAAGCGGAAAACAGTGCCGCAGCCGCAGAATCCTCACAAAACGCGGCGGACACCAGCGCCGGTGCGGCGAAAACGTCAGACACGACTGCTGCAGCGTCACAACAATCAGCCGACACGTCTGCCTTCACCGCGGCCACGAAAGCGTCAGAGGCACCCACTTCAGCACGAGATGCGGTGGCCTAAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAgtgc                                                                                                                                >  1:233429/5‑248 (MQ=255)                                                                                               gaagcGGCATCAGCAAAGGCCACTGAAGCGGAAAAAAGTGCCGCAGCCGCAGAGTCCTCAACAACCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTTCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAgtgc                                                                                                                                >  1:233441/5‑248 (MQ=255)                                                                                                                         aGCATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCAGCGTCACTACAATCAGCAGCAACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCAAACGAAGCGGCAAAATCATCAGAAACGAACGCATCATCAAGCGCCAGTAGTGCAGCTTCCTCGGCAAcgg                                                                                                      >  1:132140/1‑251 (MQ=255)                                                                                                                            ggaaaaaaGTGCCGCAGCCGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCAACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGc                                                                                                                       >  1:110498/4‑231 (MQ=255)                                                                                                                                        cgcagccgCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGGGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTctn                                                                                       <  1:55440/244‑3 (MQ=255)                                                                                                                                                 agagTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTctgccagggcgg                                                                              <  1:49061/251‑12 (MQ=255)                                                                                                                                                                        ccaccaGTGCCGGTGAGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACACTCAGCCGCCACGTCTGCCTCAAACGCGGCCACAAACGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGACCGAGACGAATGcc                                                       >  1:84057/1‑251 (MQ=255)                                                                                                                                                                        ccaccaGAGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCAGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCAAAAGAAGCGGCAAAATCATCAGAAACGAACGCATCATCAAGCGCCAGTAGTGCAGCTTCCTCGGCAAc                                                                                                        >  1:178236/1‑202 (MQ=255)                                                                                                                                                                         caccaGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCAGCGTCACTACAATCAGCAGCCACATCTGCATCCACcg                                                                                                                                                                                                                                 <  1:133021/80‑1 (MQ=255)                                                                                                                                                                                                       aaCGAATGCTTCAGCGGCACGACAATCAGCAGCCACATCGGCATCCCCCGCGACCACTAAGGCATCAGAAGCGGCGACCGCGGCCCGGGATGCGGCGGCCTCAAAAGAAGCGGCAAAATCATCAGAAGCGAACGCATCAGCAAGCGCCAGTAGTGCAGCTTCCTCGGCAACGTCGTCAGGAAATTCCACGAAGGCGTCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAgcn                        <  1:46761/251‑2 (MQ=255)                                                                                                                                                                                                                              aaTCAGCAGCCACATCTGCATCCACCGCGACCACGAAGACATCAGAAGCTGCGACCTAGGCCCGCGATACGGCGCCCTCAAAAGAAGCGACAAAATAATCCGAAACGAAAGCATCATCAAGCGCAAGTCGTGCAGCTTCCTCGGCAACGGAGGCAGGAAATTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGGGGGCCAGAGCGACTCGGCTGCGGCAGGCTCaaa  >  1:220550/1‑250 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                             AACCCATGCGGCTGATGCTGCGGACTCAGCACGCGCAGCCAGCACGTCAGCCGGACAGGCCGCGTCGTCGGCTCAGTCAGCGTCTTCCAGCGCAGGAACGGCATCAACAAAGGCCACTGAAGCATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCAGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCAAAAGAAGCGGCAAAATCATCAGAAACGAACGCATCATCAAGCGCCAGTAGTGCAGCTTCCTCGGCAACGGCGGCAGGAAATTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAA  >  NC_000913/1429474‑1429944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑