Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,560,455	+G	intergenic (+589/+167)	rtcR → / ← glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,560,455	1	.	G	100.0%	32.3 / NA	12	intergenic (+589/+167)	rtcR/glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (6/6);  total (6/6)

CGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTT‑TGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTA  >  NC_000913/3560234‑3560680                                                                                                                                                                                                                               |                                                                                                                                                                                                                                   cGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTTCGCTACCTCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGTGGATTTGCTCCGCCACTGTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACTGCCATGATGGc                                                                                                                                                                                                                                     <  1:169138/222‑1 (MQ=255)                          cAAATGGCTGTGATTGAGCAGTGCGTGGGTTGCCGCTTCCGGCTTGGTGCGGATAGCGATGAACAGCGTCGAGCCATGGGGGATTTGCTCCGCCACTTTGCGGGCTATGCGCTCTTTTTCTTCGGTCGGGGTTGCCTTGCGATCTGGCCTCGGGGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAGGGCGCAGGATCAGGTGTTTCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCg                                                                                                                                                                               <  1:97415/251‑1 (MQ=255)                                           gCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGACATTGGGGATTTGCTACGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGTCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGc                                                                                                                                                              >  1:129651/1‑251 (MQ=255)                                                                       ggtgCCGATACCGATAAACAGCGTCAGGCCATTGGGGATTTTCCCCGCCTCTTTGCGGGCGATGCGCTCTTTTTCGTCGGTCTGGGTGGCCTTGCTATCGTGCCACGGCGTTTTAACCGAACTGGAAGGCAGCGCCGCTCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACGAGCTCTTCTGTGCTGTCATAAc                                                                                                                                  <  1:183679/251‑1 (MQ=255)                                                                        gtgCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTg                                                                                                                                                                                                                     >  1:134858/1‑167 (MQ=255)                                                                                atCGATAAACAGCTTCTGGGCTTTGGGGATTTGCTGCGCCCCTTTGCGTGCGATGCGCTCTTTGTGTTCGGTCGGGGTGGCCTAGCGATCGTGCCACGGCGGGTGGACCGAACTGGAGGGCAGCCCCGCACCGCCATGATGGCGCAGTATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGtt                                                                                                                         <  1:98559/251‑1 (MQ=255)                                                                                             gTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCATGTTCACCGAACTGGAAGGCAGCGCCGCACCGACATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCGCTTCGGTACTGATATAACCCTGCTGTTTAACCAGTTCGat                                                                                                            >  1:28672/1‑251 (MQ=255)                                                                                                          ggATTTGCTCCGCCGCTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgc                                                                                                                             <  1:207045/221‑1 (MQ=255)                                                                                                                ncTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCTTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTtgt                                                                                         >  1:137838/2‑251 (MQ=255)                                                                                                                                           ctTTTTCTCTGGTCGGGGTGGCCTTGCGATCGTTCTACGTCGTGGTGACCGTACTGGAAGTCAGCGCCGCTCCGCCATGATGGCGCAGGATTAGGTTTTGCTGCGCTAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGTAGAATTGCTCTACCAGCTCTTCGGTACTGACATAACCCTTCTGGGTTACCAATTCGATAATACCGTTGTGACGTTGTGTT‑TGTTTCATTTATAAATCCCTgg                                                               <  1:167086/249‑1 (MQ=255)                                                                                                                                                                    gCGATCGTGCCCCGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTT‑TGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGc                                    <  1:176245/251‑1 (MQ=255)                                                                                                                                                                            gCCACGGANTNNNANNAGAACTGGGAGGCAGCACCGCACCGCCATGATGGCGCAGGATCCNGTTTTGCTCCGCCCGNTCNTTCCNGTCNCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATACCCATGAGGTTTAACCGGTTCGATAAAACCGTTGTAACGTGGTGTTNTGTNAC‑TTTNNANATCCCTNNna                                                             >  2:166165/1‑218 (MQ=255)                                                                                                                                                                                                       gCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTT‑TGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTa  >  1:156500/1‑250 (MQ=255)                                                                                                                                                                                                                               |                                                                                                                                                                                                                                   CGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTT‑TGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTA  >  NC_000913/3560234‑3560680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑