Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	64,677	A→T	G368G (GGT→GGA)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	64,677	0	A	T	88.9%	10.5 / ‑6.8	10	G368G (GGT→GGA)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base A (1/0);  major base T (4/4);  minor base G (1/0);  total (6/4)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGCACTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGA‑ACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCA  >  NC_000913/64452‑64850                                                                                                                                                                                                                                   |                                                                                                                                                                              cgcCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGGGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCGGGCGTGTGGCGGCACTTCGCCGAGATTATGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGA‑TCCCCCGTGGCGGTCTACCGGCAggn                                                                                                                                                      <  1:28278/251‑2 (MQ=255)                              caatcaGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGATATGCCCAAACGCCGCCACCGA‑TCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGACGCCCGTTCGAGTAAAAAt                                                                                                                         >  1:14254/1‑251 (MQ=255)                                            cGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACTAGTACCGAATCATAAAGCTCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGAGGACCAAACGCCGCCGCCGA‑TC‑‑CCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGttt                                                                                                         <  1:137944/251‑1 (MQ=255)                                                     gACGGGTAAAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGACGCGAATCCATTACGTAGCAGCCAGGGCTGGCGTGTGGACGAACTTCGCCGAGATTAGGAGAGACATAACCCGCGCGGTGCATACGCGGACAATTGAGATGACCAAACGCCGCCCCCGC‑TCCCCCGTGGCGGTCTACCGGCAGGCCGTTAACCGTCCCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATggc                                                                                                   >  1:272802/1‑249 (MQ=255)                                                                                      accgAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGA‑TCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGATTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCGTTCAGGt                                                                  <  1:96202/247‑1 (MQ=255)                                                                                                   gCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACCTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGC‑TCCCCCGTGGCGGTCTACCGGccg                                                                                                                                                        >  1:246948/1‑149 (MQ=255)                                                                                                            gTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCCCTTCGCCGCGATTAGCAGCGACATAACCAGCGCGGTGCATACGAGGACACTAGAGATGACCAAAAGCCGACACCGA‑GCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGCAAATCTGCGTCACCAGCTCGCAATCTTTCAGGGTATAAGTCGCCAGCGCAGGttt                                            >  1:115821/1‑250 (MQ=255)                                                                                                                                                gtggcACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGA‑TCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCg        >  1:229110/3‑250 (MQ=255)                                                                                                                                                     aCTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCTCCGA‑TCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCa  <  1:224170/251‑1 (MQ=255)                                                                                                                                                     aCTTCGCCGAGATTAGGCGCGACATAAACAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACAGATACC‑CCGTGGCGGTCTAACGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAACGACATGATTTCAGTTTTATGGAAAATCTGCGTCACAAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCAGACAATTTCGTCCATTCGATccc   >  1:199325/1‑250 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                              CGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGCACTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGA‑ACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCA  >  NC_000913/64452‑64850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑