Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,441,201	A→G	I5I (ATT→ATC)	paeA ←	putative cadaverine/putrescine exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,441,201	0	A	G	100.0%	52.8 / NA	15	I5I (ATT→ATC)	paeA	putative cadaverine/putrescine exporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/8);  total (7/8)

TGTGAAAAGCTGGAGAAAATGCCGCATCACCGACGATACCGCCGAGAATCGCCACTGCGTTCAGACCGATTTGGACCACGGTAAAGAACATGCCGGGATTTTCCTGCATATTCAGAACGCGTTGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTACAGCGATCAAGCAGAGTATGACTAAAATACTGTTTAACATATCTTATCCGACCTTTCAGGCCAGATCCTCGGAAGGGGAAGTTGATTAATTTGTGTGAAATACACATTGAAAGCCGATTCGAAGAAAGTGAACCGGCATTTTCAGTGGCTAGTATAGCGTAAGGTACTGTAAAGCCGCCAGAGGGTTAAAATTCAGACAGCTGAAAAATGCAAAACTGCCTGATACGCTATGCCGATCAGGCCTACGCAGTCCTTGCAATAT  >  NC_000913/4440960‑4441437                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                             tgtgAAAAGCTGGAGAAAATGCCGCATCACCGACGATACCGCCGAGAATCGCCACTGCGTTCAGACCGATTTGGACCACGGTAAAGAACATGCCGGGATTTTCCTGCATATTCAGAACGCGTTGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGATATCACGTACATCGAGAAGAACGCACTTACAGCGATCAAGCAGAGTATGACTAAGATACTGtt                                                                                                                                                                                                                                      <  1:110663/250‑1 (MQ=255)                                                   gCCACTGCGTTCAGACCGATTTGGACCACGGTAAAAAACATGCCGGGATTTTCCTGCATATTCAGAACGCGTTGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTACAGCGATCAAGCAGAGTATGACTAAGATACTGttn                                                                                                                                                                                                                                     <  1:80990/201‑2 (MQ=255)                                                                         ngACCACGGTAAAGAACATGCCGGGATTTTCCTGCATATTCAGAACGCGTTGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTACAGCGATCAAGCAGAGTATGACTAAGATACTGTTTAACATATCTTATCCGACCTTTCAGGCCAGATCCTCGGAAGGGGAAGTTGATTAATTTGTGTGAAATACACAt                                                                                                                                                             >  1:165740/2‑251 (MQ=255)                                                                                                                                        tGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTACAGCGATCAAGCAGAGTATGACTAAGATACTGTTTAACATATCTTATCCGACCTTTCAGGCCAGATCCTCGGAAGGGGAAGTTGATTAATTTGTGTGAAATACACATTGAAAGCCGATTCGAAGAAAGTGAACCGGCATTTTCAGTGGCTAGTATAGCGTAAGGTACTGt                                                                                              >  1:7282/1‑251 (MQ=255)                                                                                                                                                                          gCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTACAGCGATCACGCAGAGTATGACTAAGATACTGTTTAACATATCTTATCCGACCTTTCAGGCCAGATCCTCGGAAGGGGAAGTTGATTAATTTGTGTGAAATACACATTGAAAGCCGATTCGAAGAAAGTGAACCGGCATTTTCAGTGGCTAGTATAGCGTAAGGTACTGTAAAGCCGCAAGAGGGTTAAAATTCAGACAGCTg                                                             >  1:264613/1‑250 (MQ=255)                                                                                                                                                                                 gcggcAAGCGAGATCTCGGACATCGAGAAGAACGCACTTACAGCGATCAAGCAGAGTATGACTAAGATACTGTTTAACATATCTTATCCGACCTTTCAGGCCAGATCCTCGGAAGGGGAAGTTGAt                                                                                                                                                                                  >  1:60809/1‑126 (MQ=255)                                                                                                                                                                                 gcggcAAGCGAGATCTCGGACATCGAGAAGAACGCACTTACAGCGATCAAGCAGAGTATGACTAAGATACTGTTTAACATATCTTATCCGACCTTTCAGGCCAGATCCTCGGAAGGGGAAGTTGATTAATTTGTGTGAAATACACATTGAAAGCCGATTCGAAGAAAGTGAACCGGCATTTTCAGTGGCTAGTATAGCGTAAGGTACTGTAAAGCCGCCAGAGGGTTAAAATTCAGACAGCTGAAAAATgn                                                     <  1:166769/251‑2 (MQ=255)                                                                                                                                                                                 gcggcAAGCGAGATCTCGGACATCGAGAAGAACGCACNTACAGCGATNNAGNAGAGGNTNNNTNNNATNCTGTTTAACATATCTTATCNGANNTTTCAGNCCAGATCCTCGGAAGGGGAAGTTGAt                                                                                                                                                                                  <  2:60809/126‑1 (MQ=255)                                                                                                                                                                                                 cGGACNNCGAGAAGAACGCACTTACAGCGATCAAGCAGAGTATGACTAAGATACTGTTTAACATATCTTATCCGACCTTTCAGGCCAGATCCTCg                                                                                                                                                                                                 <  2:19982/95‑1 (MQ=255)                                                                                                                                                                                                   gACATCGAGAAGAACGCACTTACAGCGATCAAGCAGAGTATGACTAAGATACTGTTTAACATATCTTATCCGACCTTTCAGGCCAGATCCTCGGAAGGGGAAGTTGATTAATTTGTGTGAAATACACATTGAANGcc                                                                                                                                                     >  2:162752/1‑137 (MQ=255)                                                                                                                                                                                                   gACATCGAGAAGAACGCACTTACAGCGATCAAGCAGAGTATGACTAAGATACTGTTTAACATATCTTATCCGACCTTTCAGGCCAGATCCTCGGAAGGGGAAGTTGATTAATTTGTGTGAAATACACATTGAAAGcn                                                                                                                                                     <  1:162752/137‑2 (MQ=255)                                                                                                                                                                                                           gaagaaCGCACTTACAGCGATCAAGCAGAGTATGACTAAGATACTGTTTAACATATCTTATCCGACCTTTCAGGCCAGATCCTCGGAAGGGGAAGTTGATTAATTTGTGTGAAATACACATTGAAAGCCGATTCGAAGAAAGTGAACCGGCATTTTCAGTGGCTAGTATAGCGTAAGGTACTGTAAAGCCGCCAGAGGGTTAAAATTCAGACAGc                                                               <  1:254090/215‑1 (MQ=255)                                                                                                                                                                                                                    aCTTACAGCGATCAAGCAGAGTATGACTAAGATACTGTTTAACATATCTTATCCGACCTTTCAGGCCAGATCCTCGGAAGGGGAAGTTGATTAATTTGTGTGAAATACACATTGAAAGCCGATTCGAAGAAAGTGAACCGGCATTTTCAGTGGCTAGTATAGCGTAAGGTACTGTAAAGCCGCCAGAGGGTTAAAATTCAGACAGCTGAAAAATGCAAAACTGCCTGATACGCTATGCCGATCAGGCCTAc                  <  1:235992/251‑1 (MQ=255)                                                                                                                                                                                                                           gCGATCAAGCAGAGTATGACTAAGATACTGTTTAACATATCTTATCCGACCTTTCAGGCCAGATCCTCGGAAGGGGAAGTTGATTAATTTGTGTGAAATACACATTGAAAGCCGATTCGAAGAAAGTGAACCGGCATTTTCAGTGGCTAGTATAGCGTAAGGTACTGTAAAGCCGCCAGAGGGTTAAAATTCAGACAGCTGAAAAATGCAAAACTGCCTGATACGCTATGCCGATCAGGc                      <  1:6539/240‑1 (MQ=255)                                                                                                                                                                                                                           gCGATCAAGCAGAGTATGACTAAGATACTGTTTAACATATCTTATCCGACCTTTCAGGCCAGATCCTCGGAAGGGGAAGTTGATTAATNNGTGTGANNt                                                                                                                                                                   >  2:6539/1‑99 (MQ=255)                                                                                                                                                                                                                                    cAGAGTATGCCTAAGATACTGTTTAACATATCTTATCCGCCCTTTCAGGCCAGATCCTCGGAAGGGGAAGTTGATTAATTTGTGTGAAATACACATTGAAAGCCGATTCGAAGAAAGTGAACCGGCATTTTCAGTGGCTAGTATAGCGTAAGGTACTGTAAAGCCGCCAGAGGGTTAAAATTCAGACAGCTGAAAAATGCAAAACTGCCTGATACGCTATGCCGATCAGGCCTACGCAGTCCTTGCAatat  >  1:221449/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                             TGTGAAAAGCTGGAGAAAATGCCGCATCACCGACGATACCGCCGAGAATCGCCACTGCGTTCAGACCGATTTGGACCACGGTAAAGAACATGCCGGGATTTTCCTGCATATTCAGAACGCGTTGGGCATTTATATTGCCTTCATCAGCCAGCAGTTTAAGTTTGATTTTGCGTGAGGCGGCAAGCGAGATCTCGGACATCGAGAAGAACGCACTTACAGCGATCAAGCAGAGTATGACTAAAATACTGTTTAACATATCTTATCCGACCTTTCAGGCCAGATCCTCGGAAGGGGAAGTTGATTAATTTGTGTGAAATACACATTGAAAGCCGATTCGAAGAAAGTGAACCGGCATTTTCAGTGGCTAGTATAGCGTAAGGTACTGTAAAGCCGCCAGAGGGTTAAAATTCAGACAGCTGAAAAATGCAAAACTGCCTGATACGCTATGCCGATCAGGCCTACGCAGTCCTTGCAATAT  >  NC_000913/4440960‑4441437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑