Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,350,649	G→A	noncoding (73/121 nt)	arcZ →	small regulatory RNA ArcZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,350,649	0	G	A	100.0%	28.3 / NA	11	noncoding (73/121 nt)	arcZ	small regulatory RNA ArcZ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/7);  total (4/7)

AACCGTCATAGACGCCGCATCCGCTCAGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGAAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTTAACTGATTCATGTAACAAATCATTTAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACC  >  NC_000913/3350411‑3350865                                                                                                                                                                                                                                               |                                                                                                                                                                                                                         aaCCGTCATAGACGCCGCATCCGCTCAGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGAAACACATTAATTTTTTTAATAAAAATGGTACGCATCTCACATTTAACTGATTCATGTAACAAATCATTTAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAg                                                                                                                                                                                                              <  1:249484/251‑1 (MQ=255)                                                                       gACTGAAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTAAACTGATTCATGTAACACATCATTTAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCAcc                                                                                                                                                                                                  >  1:144362/1‑193 (MQ=255)                                                                                     aaTTTTTTTAATAAAAATGGTACGCATCACACATTTAACTGATTCATGTAACAAATCATTTAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGcn                                                                                                                           <  1:50161/250‑2 (MQ=255)                                                                                      aTTTTTTTAATAAAAATGGTACGCATCACACATTTAACTGATTCATGTAACAAATCATTTAAGGTTTGCTATCTTAACGGCGTGCGGCCTGAAAAACAGGGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTAGTGTGGGCGCAGTATTCGCGCACCCCGGGCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCTCCCACGCTTTCAGCACTTCTACGTCGTGACGc                                                                                                                            <  1:123852/248‑1 (MQ=255)                                                                                                 aaaaaTGGTACGCATCACACATTTAACTGATTCATGTAACAAATCATTTAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGc                                                                                                                                                              <  1:144591/203‑1 (MQ=255)                                                                                                                         naCTGATTCATGTAACAAATCATTTAAGTTTTGGTATCTTAACTGCGTGCGGACTGAAAAACAGTGCTGTGCCCTTGTACCTCATCATAATAATTTACGGAGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGATTTTGCCACCCACGCTGTCAGCACTTCTACGTCGAGACGCAACTCTTCTTTCATCTCTTCAATCCATTCACCGACGtt                                                                                      >  1:33914/2‑251 (MQ=255)                                                                                                                                                         gCTATCGTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATGTACGGCGCAGCCAAGATTTCCCTATTGTTGGCGCAGTATTCGCGCACGCCGGTCTAGCCGGGGTCATCTTTTATTGGCTTTTGCCACCCACGCTTTCAGCAGTTCTACGTCGTGACGCCACTCTTCTTGCATCTCTTCAATCCATTCACCGACTTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGa                                                       <  1:238737/250‑1 (MQ=255)                                                                                                                                                                      cgtgcgGCCTGAAAAACAGTGCTAATCCCTTGTAACTCATCATAATAATTTACGGCGCAGACACGATTTCCCTAGTGTTGGCGAAGTATTCGCGCACCCCGGTCTAGCCGTGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCCGCACTTCTACGTCGTGACGCCCCTCTTCTTTCATCTCTTCAATCCATTCACCGTCGTTCTCTTCCCATGCCGGCAGGGCAGGAGACTGCATTTGCTGAc                                             >  1:95000/1‑247 (MQ=255)                                                                                                                                                                          cgGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTTAATTTGCTGACCCAGCTGt                                     <  1:95944/251‑1 (MQ=255)                                                                                                                                                                                                                         aCGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACTCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTAGCTTCCCAGGcc                                                                         <  1:214886/168‑1 (MQ=255)                                                                                                                                                                                                                                   naaGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCAcc  >  1:163802/2‑229 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                         AACCGTCATAGACGCCGCATCCGCTCAGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGAAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTTAACTGATTCATGTAACAAATCATTTAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACC  >  NC_000913/3350411‑3350865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑