Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,905,761	G→A	G25D (GGT→GAT)	mntP →	Mn(2(+)) exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,905,761	0	G	A	100.0%	27.8 / NA	10	G25D (GGT→GAT)	mntP	Mn(2(+)) exporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/5);  total (5/5)

TGGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCGTCGAC  >  NC_000913/1905529‑1905994                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                          tGGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCAccc                                                                                                                                                                                                                                   <  1:43300/241‑1 (MQ=255)  ggCACGTACGGACTGAATTCTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGGTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAAcc                                                                                                                                                                                                                         <  1:203206/250‑1 (MQ=255)   ncACGTACGGACTGCATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCg                                                                                                                                                                                                                        >  1:71577/2‑250 (MQ=255)            gACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGGGTTATGGATACTCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTGCTGGCGTTTGGTAGGTCGATGGATGCAGTTGCTGCATCAATCGGTAAAGATACCACCCTCCATAAACCGAAATTTTCTg                                                                                                                                                                                                              <  1:152421/251‑1 (MQ=255)                                                                    gAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGGTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGGTTGGTATGTCGATGGAAGCATTTGCTGCATCAATCGGTAAAGATGCTACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGAtn                                                                                                                                                      <  1:27115/251‑2 (MQ=255)                                                                                                                                                      ggACATTGTCATGAATCTCACTGCTACTGTTCTGCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCACTCTGTACAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACAGACCTTATTTTTgg                                                                                                                                                                                  >  1:58049/1‑141 (MQ=255)                                                                                                                                                      ggACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGNTGGATGCATNNGCNGCATTNANNNGNNNAGNTGCCACCCTCCATAAACCGNAANNTTCTGANGCATTGCGAACCGGCCTTATTTTTgg                                                                                                                                                                                  <  2:58049/141‑1 (MQ=255)                                                                                                                                                                     tatCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGNNATTTTCNNa                                                                                                                                                                                                             >  2:8330/1‑99 (MQ=255)                                                                                                                                                                     tatCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCggcgggc                                                              <  1:8330/242‑1 (MQ=255)                                                                                                                                                                                                                       ctgcATCAATCGGTAAAGATGCCACCCTCCATAAAcc                                                                                                                                                                                                                         >  1:101147/1‑37 (MQ=255)                                                                                                                                                                                                                         gcATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGACTGGGCATGTTAGCCAGCCGGTTTGTCCTTGACTGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGAGGCGCAGATGATGAAGATGAAGAGCCGCGCCGTCGac  >  1:209580/1‑250 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                          TGGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCGTCGAC  >  NC_000913/1905529‑1905994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑