Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,776,715	A→T	S377C (AGT→TGT)	ydiF →	putative acetate‑CoA transferase YdiF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,776,715	0	A	T	92.3%	28.5 / NA	13	S377C (AGT→TGT)	ydiF	putative acetate‑CoA transferase YdiF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (6/6);  minor base G (0/1);  total (6/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGAAATGCGTAAAGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGAGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGACGGCAAATT  >  NC_000913/1776477‑1776881                                                                                                                                                                                                                                               |                                                                                                                                                                       cGAAATGCGTAAAGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCGTTGGCGCGAACGTGAATACCCGTTCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTTGCGGTGTGGATTTTTGTTATTTGTGTTATGCTGAAg                                                                                                                                                            <  1:231263/251‑1 (MQ=255)                             gTCGGCGTCGGTATTGCTGACGGCATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTgn                                                                                                                                 <  1:81802/250‑2 (MQ=255)                                                                        aagaagGTTGTGCTGATGACTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGGCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGGTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATGCAATGGTAAAATCATGGGCACCGGTGGATTTATTGat                                                                                     <  1:239463/251‑1 (MQ=255)                                                                                                 tCTGACGGTAGAAACAGGTCTGATTGGCGGAATGACTTCCCGGGTGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTTATTTGTATCCCGGTGGCGGTCTGGATGTTTTTTATTTGTGTTTACCTGAATTCGACCATCACTTTAACGTTGGCGTGGTTAAATTCAATTGTAAAATCATGGGCTTCGGTAGATTTATTGATATCAGTTCCACTTCGATGAAAAtn                                                             <  1:69032/250‑2 (MQ=255)                                                                                                                          ggCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCgg                                    <  1:92152/250‑1 (MQ=255)                                                                                                                           gCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCAtt                                                          >  1:99774/1‑227 (MQ=255)                                                                                                                             ggAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTGGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCTGCGTGCATAAATTCAATGGTAAAATCATgg                                                                                                         <  1:101955/178‑1 (MQ=255)                                                                                                                                             nnnncNCCNNTGGCNCGAACGTGAATACCCGTGCCATTCNGGATATGACGTCCCATTTTGAGGTTTAGCACNGNGGCTGTCTGGATGTTTGTTATTTGGGTTTTGCTGAAGTCGACCAGCACNGTAACGTCGGNGTGNNTAAANTNANTGGTAAAATCATGGGCACCGGTGGATTTANTGATATCAGTGCCACTTCGAAGANNTTNNNTTNNTGCgg                                                  <  2:200236/213‑1 (MQ=255)                                                                                                                                                   ccTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATACCGGTGGCGGTCTGGATGTTTGTTCTTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCACTGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATAATTTTCTGCgg                                                  >  1:200236/1‑211 (MQ=255)                                                                                                                                                         gcgcGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTNAATNNNnna                                                                                                                >  2:167801/1‑143 (MQ=255)                                                                                                                                                           gcgAACGTGAATACCCGTGCCATTCAGGATATGCAGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTCACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGACGGCAAAtt  >  1:220739/1‑251 (MQ=255)                                                                                                                                                            cgAACGTGAATAACCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCgg                                                                                                  >  1:213659/1‑154 (MQ=255)                                                                                                                                                                                        tatGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTATGGATGTTTGTTATTTGTGTTTTGCTgtagcc                                                                                                                                                          >  1:270775/1‑65 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                       CGAAATGCGTAAAGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGAGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGACGGCAAATT  >  NC_000913/1776477‑1776881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑