Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	803,662	C→A	L54I (CTT→ATT)	ybhJ →	putative hydratase YbhJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	803,662	0	C	A	100.0%	44.7 / NA	16	L54I (CTT→ATT)	ybhJ	putative hydratase YbhJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (9/7);  total (9/7)

CCTTCCTGCAACTCGAATTATTTAGAGTATATCCATTTATTATCTTTCTGCGCACTTCACGGTGCGCAGATATCTGGAGCATTTGATGATCAAGTTATCTGAAAAAGGCGTGTTTCTCGCCAGTAATAACGAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAACTTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTCATCCATCAAT  >  NC_000913/803418‑803899                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                              ncTTCCTGCAACTCGAATTATTTAGAGTATATCCATTTATTATCTTTCTGCGCACTTCACGGTGCGCAGATATCTGGAGCATTTGATGATCAAGTTATCTGAAAAAGGCGTGTTTCTCGCCAGTAATAACGAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTaaa                                                                                                                                                                                                                                          >  1:79720/2‑250 (MQ=255) ccTTCCTGCAACTCGAATTATTTAGAGTATATCCATTTATTATCTTTCTGCGCACTTCACGGTGCGCAGCTATCTGGAGCATTTGATGATCAAGTTCTCTGAAAAAGGCGTGTTTCTCGCCAGTAATAACGAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCAAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCCTAATACGTCCGGAAATATGGATAAAATTaaa                                                                                                                                                                                                                                          >  1:122084/1‑250 (MQ=255)                                                    gcACTTCACGGTGCGCAGATATCTGGAGCATTTGATGATCAAGTGATCTGAAAAAGGCGTGTTTCTCGCCAGTAATAACGAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAAGTAAGTTTGATTCGTTAGCCTCTCACGATATTACCTTTGTTGGTATTGTAc                                                                                                                                                                                      <  1:237293/251‑1 (MQ=255)                                                                                                         aaGGCGTGTTTCTCGCCAGTAATAACGAAATAATTGCCGAAGAACATTTCACCGTCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCTTTAGCCTCTCACGATATTACCGTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGAcc                                                                                                                                 <  1:189679/251‑1 (MQ=255)                                                                                                                      cGCCAGTAATAACGAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGCATCCACAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGATTGATTCATTAGCCTCTCACGATATTAACTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAact                                                                                                                    >  1:200415/1‑251 (MQ=255)                                                                                                                                            gCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCAc                                                                                              >  1:65155/1‑251 (MQ=255)                                                                                                                                               gaagaaCATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAACAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGACATATGGATAAAATTAAAATTAAGTTTGAGTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGCCCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTAt                                                                                           >  1:204921/1‑251 (MQ=255)                                                                                                                                                    aCATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTGCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTAGTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACg                                                                                      <  1:202913/251‑1 (MQ=255)                                                                                                                                                           aCCGGCGAAATTAAAAAAGAAGAAGACAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAAGACGTCCGGAAATATGGAGAAAATTAAAATTAAGTTGGATTCATTAGCCTCTCACGATATTACCTTTGTGGGTATTTGACAGACCGCTAGAGGGGCCGGTAGGGAACGTTTCCCGCTTCCGTATGTGCAGACCAACTGCCATAAGCCACTCTGCGCCGTCGGCGGCACTATTAACGGtgatga                                                                               <  1:121604/251‑1 (MQ=255)                                                                                                                                                                                    ccAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGAATAATACGTCCGGAAATATGGATACAATTAAAATTACGTTTGATTCATTGGCCTCTCACGATATTACCTTTGTTGGCATTGTACAGACCGCTAAAGCGTCCGGGATGGAACGTTTCCCGCTGCCGTATGTGCTTACCAACTCCAATAACTCCCTCTGCGCCGTc                                                                                                      >  1:92406/1‑203 (MQ=255)                                                                                                                                                                                              cACTATTGCCTGGTCTATTCTCTCTTNGCATAANNCGNCCGGAAATATGGATAAAATNAANNNTNNNTNTGATTNATNNGCCTCTCACNATATTACCTTTGTTGGTATTGTACAGAc                                                                                                                                                                                  >  2:64407/1‑117 (MQ=255)                                                                                                                                                                                              cACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGAc                                                                                                                                                                                  <  1:64407/117‑1 (MQ=255)                                                                                                                                                                                                     gCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAACTATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAg                                                                                                                                                                          >  1:114868/1‑118 (MQ=255)                                                                                                                                                                                                     gCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTATCCTCTCACGATATTACCTGTGTTGGTATTGTACAGACCGCGAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTgcg                                                                                                           <  1:186519/181‑1 (MQ=255)                                                                                                                                                                                                            cTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTAt                                             >  1:116750/1‑236 (MQ=255)                                                                                                                                                                                                                                        aaaTATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCTCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACGATTAACGGTGATGACCATGGTTTTGGTTTATCGGCAGCCCATCGTTATTGCGGTATTTTTGTGCCTCCGCATATTGCGGGCATCCATCAat  <  1:241325/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                              CCTTCCTGCAACTCGAATTATTTAGAGTATATCCATTTATTATCTTTCTGCGCACTTCACGGTGCGCAGATATCTGGAGCATTTGATGATCAAGTTATCTGAAAAAGGCGTGTTTCTCGCCAGTAATAACGAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAACTTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTCATCCATCAAT  >  NC_000913/803418‑803899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑