| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 94,278 | G→A | A371A (GCG→GCA) | murE → | UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate‑‑2, 6‑diaminopimelate ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 94,278 | 0 | G | A | 100.0% | 44.0 / NA | 16 | A371A (GCG→GCA) | murE | UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate‑‑2, 6‑diaminopimelate ligase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (6/10); total (6/10) | |||||||||||
GAAATTGAAAGCCATCTGATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGCGACACTGTTGGCACTCGGCTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCGGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGATGGGCGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCATCATCAACGATATTCTGGCGGGAATGTTAGATGCCGGACATGCCAAAGTGATGGAAGGCCGTGCTGAAGCGGTGACTTGCGCCGTT > NC_000913/94057‑94521 | gAAATTGAAAGCCATCTGATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGCGCCACTGTTGGCACTCGGCTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCtgtg > 1:176873/1‑251 (MQ=255) ggCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGCGACTCTGTTGGCACTCGGCTATACACTGGCTGATATGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGCACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGGGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCAATGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGtggtgg > 1:262976/1‑251 (MQ=255) ggCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGCGACACTGTTGGCACTCGGCTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCtgtgn < 1:162521/231‑2 (MQ=255) tAACGTCAGCAACCTGCTGCTCGCGCTGGCGACACTGTTGGCACTCGGCTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAAGCGGTTTGTGGACGGATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTTGATGAGGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGGGTGTCTGTGGCTGTGGTGTCGATcgc < 1:190582/250‑1 (MQ=255) cTCGGCTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAAACGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCACACCGACGGTGGCGGTGGATTACGCGCATACGCCGGCTGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTC‑ACTGATGGGCGCAATTGCCGAAGAGttt > 1:257942/1‑251 (MQ=255) cACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGGCCACTGATGGGCGCAATTGCCGAAGAGTTTGCTGACGTg < 1:107936/251‑1 (MQ=255) ggCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGTTAAGCGTCCACTGATGGGCGCAATTGCCGAAGAGTTTGCTGGCGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGc < 1:245653/250‑1 (MQ=255) aCTGCGCCNGNNNANNAGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAANAAACCTTACAGGCAGNGCNTCTGNACTNTGCGGGCAAGCTGTGGTGTGTCTTTGGCtg > 2:197574/1‑119 (MQ=255) aCTGCGCCAGTCAAACCGACGGTGGTGGTGTATTACGCGCATTCGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCtg < 1:197574/119‑1 (MQ=255) cAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCg < 1:184078/116‑1 (MQ=255) gACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGATGGGCGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCATCATCAACGATATTCTGGCGGGAATGTTAGATGCCg < 1:150041/251‑1 (MQ=255) tACGCGNNTACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGAt < 2:20800/96‑1 (MQ=255) tACGCGCATACGCAGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGAt > 1:20800/1‑96 (MQ=255) ccTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGATGGGCGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCATCATCAACGATATTCTGGCGGGAATGTTAGGTGCCGGACATGCCAAAGGGATGGAAGGCCGGGCTGAAGc > 1:74956/1‑238 (MQ=255) ccTTACAGGCAGCGCGGCTGCTCTGTTCTGGCAATCTGTGGTGTGTCTGGGGCTGTGGTGGCGATCTCGAGAAATGTAAGCGCCCACTGATGGTCGCAATTGCCGAAGAAGTTGCTGACGGAGCGGCGGTGACGGACGAGAACCCGCGTACCGAAGAACCGCATGCCATCATCAACGATAATCTGCCGGTAATTTTAGATGCCGGACATGCCAAAGTGATGGAAGGCCGTGCTGAAGCGGAGACTTGCGcc < 1:140067/251‑1 (MQ=255) aCAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGATGGGCGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCATCATCAACGATATTCTGGCGGGAATGTTAGATGCCGGACATGCCAAAGTGATGGAAGGCCGTGCTGAAGCGGTGACTTGCGCCGtt < 1:3588/250‑1 (MQ=255) | GAAATTGAAAGCCATCTGATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGCGACACTGTTGGCACTCGGCTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCGGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGATGGGCGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCATCATCAACGATATTCTGGCGGGAATGTTAGATGCCGGACATGCCAAAGTGATGGAAGGCCGTGCTGAAGCGGTGACTTGCGCCGTT > NC_000913/94057‑94521 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |